Fig. 5 | Nature Communications

Fig. 5

From: Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit

Fig. 5

Human SC-NES cells form a relay system in the injured SC. a Hindlimb locomotion: BMS scores before and after dorsal SC hemisection in animals subject to cell implant (n = 19) and in controls (n = 14). The first time point indicates the locomotor performance one week after injury and three days prior to cell engraftment. b Spared tissue quantification in control and treated animals. c Correlation analysis of spared tissue percentage and the BMS score at 8 weeks in a subgroup of animals. Each symbol represents an animal. The correlation shows a significant difference between SC-NES cell recipient animals and the control group. The blue line indicates the percentage of spared tissue equal to 25%. Animals on the right of the blue line were selected for a stratification of the original cohort of animals shown in a. d BMS scores including SC-NES cells recipient animals (n = 6) and control animals (n = 6) with a percentage of spared tissue greater than 25%. e BMS score of SC-NES cells recipient animals at 8 weeks. Each dot represents an animal. SC-NES cells were transduced with the hM4Di-mcherry construct prior to surgical implantation. Animals were scored before CNO administration (Pre-CNO), 30 min after CNO administration (CNO), and 24 h after (wash-out). f The per-animal change in BMS score before and 30 min after CNO administration is plotted for SC-NES cell-treated and control animals. g Grid-walking test: percentage of missed steps of SC-NES cell recipient animals before CNO treatment, under CNO effect and 24 h after drug administration (wash-out). h Schematics of the foot print test. Base of support and stride length were measured. i Representative images of SC-NES cell recipient animals footprints before, during and after CNO injection. Black arrows indicate the stride length for one representative step in all three conditions. j Quantification of stride length in SC-NES cell recipient animals pre-CNO, upon CNO treatment and after drug wash-out. k Quantification of base of support distance. Data are expressed as means ± s.e.m. (*P < 0.05; **P < 0.01; Student’s t-test for comparisons between two groups and repeated measures ANOVA for BMS tests)

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