Table 1 Haplotypes of pfcrt-edited and control P. falciparum lines

From: Emerging Southeast Asian PfCRT mutations confer Plasmodium falciparum resistance to the first-line antimalarial piperaquine

   PfCRT haplotype at listed position
Parasite line Edited pfcrt 72 74 75 76 97 144 145 148 194 220 271 326 333 343 353 356 371
Dd2 No C I E T H A F L I S E S T M G T I
Dd2Dd2 crt Yes C I E T H A F L I S E S T M G T I
Dd2Dd2 crt F145I Yes C I E T H A I L I S E S T M G T I
Dd2Dd2 crt M343L Yes C I E T H A F L I S E S T L G T I
Dd2Dd2 crt G353V Yes C I E T H A F L I S E S T M V T I
PH1008-C No C I E T H A F L I S E S T L G T I
PH1008-CDd2 crt Yes C I E T H A F L I S E S T M G T I
PH1263-C No C I E T Y A F L I S E S T M G T I
PH1263-CDd2 crt Yes C I E T H A F L I S E S T M G T I
Cam734 No C I D T H F F I T S E N S M G I R
GB4 No C I E T H A F L I S E N T M G I I
3D7 No C M N K H A F L I A Q N T M G I R
  1. pfcrt was edited using customized zinc finger-nucleases, and clones were generated by limiting dilution. Differences from the 3D7 wild-type allele are shown in italics for Dd2 and bold for Cam734 mutations. Underline indicates mutations identified from piperaquine-resistant field isolates and tested herein by gene editing