Redox cancer therapeutics target the increased reliance on intracellular antioxidant systems and enhanced susceptibility to oxidant-induced stress of some cancer cells compared to normal cells. Many of these therapeutics are thought to perturb intracellular levels of the oxidant hydrogen peroxide (H2O2), a signaling molecule that modulates a number of different processes in human cells. However, fluorescent probes for this species remain limited in their ability to detect the small perturbations induced during successful treatments. We report a fluorescent sensor based upon human peroxiredoxin-2, which acts as the natural indicator of small H2O2 fluctuations in human cells. The new probe reveals peroxide-induced oxidation in human cells below the detection limit of current probes, as well as peroxiredoxin-2 oxidation caused by two different redox cancer therapeutics in living cells. This capability will be useful in elucidating the mechanism of current redox-based therapeutics and in developing new ones.
Hydrogen peroxide (H2O2) can regulate a variety cellular processes via the oxidation of specific cysteine residues of redox-regulated proteins, which can trigger a range of cellular responses, including cell growth and proliferation at lower levels of H2O2, as well as autophagy, apoptosis, and necrosis at higher levels of this oxidant1,2,3,4,5. Many cancer cells are thought to have much higher rates of production of H2O2 compared to normal cells due to a combination of both aberrant metabolism and genetic mutations6,7. As a result of this additional H2O2 production, many of these cells are thought to have higher susceptibility to H2O2-induced apoptosis compared to normal cells8,9,10,11,12. In order to combat this increase in oxidant production, cancer cells also upregulate key antioxidant species in order to remove this oxidant from the cell and ensure their survival6,8. Redox cancer therapeutics target cancer cells’ increased dependence on intracellular antioxidant systems and enhanced susceptibility to H2O2-induced stress through inhibition of these antioxidant systems that detoxify the cell, resulting in elevation of oxidants within the cell8,9,10,11,12. Several small molecule cancer therapeutics, such as piperlongumine13, are thought to result in elevated intracellular H2O2 that is central to their toxicity mechanisms; however, due to the small perturbations produced in response to several of these drugs, it remains difficult to detect H2O2 fluctuations in response to these treatments14.
In human cells, cytosolic H2O2 levels are controlled by a powerful network of specific reductive reactions primarily accomplished by peroxiredoxins (Prxs), thioredoxin (Trx), and thioredoxin reductase (TrxR), with reducing equivalents supplied by nicotinamide adenine dinucleotide phosphate (NADPH)15,16. Winterbourn et al.2 noted that human Prxs should scavenge the majority of H2O2 in human cells on the basis of their second-order rate coefficients for reaction with H2O2 (on the order of 1 × 107 M−1s−1)17 and abundance in the cytosol of mammalian cells (on the order of 1 × 10−4 M)16,18. Further analysis of this pathway with detailed kinetic models revealed that H2O2 clearance models that only consider H2O2 consumption by Prxs and neglect all other antioxidants produce the same clearance curves for all reasonable levels of H2O2 as those predicted by a full metabolic model of H2O2 clearance16. One abundant isoform, Prx2, achieves this high reaction rate via the exceptionally reactive thiol group of a cysteine residue near the N-terminus of the protein known as the catalytic cysteine19,20. When the catalytic cysteine is oxidized to a sulfenic acid, it forms a disulfide bond with a second cysteine residue, known as the resolving cysteine, of an adjacent Prx2 monomer. This process involves a conformational change and results in a covalently-linked dimer species. After dimer formation, Trx reduces the disulfide bond between the two Prx2 monomers and regenerates the original proteins, while TrxR reduces oxidized Trx using electrons from NADPH19,20. This set of reactions maintains a large amount of reduced Prx2 in the cytosol of cells16, poised to respond to very small changes in cytosolic H2O2 fluxes. This feature of the system suggests that Prx2 oxidation could serve as an indicator for fluctuations in physiological levels of H2O2, and that human Prx2 fused with one or more fluorescent proteins could serve as the basis of a specific and sensitive sensor for H2O2.
Currently, the most common way to measure H2O2-induced Prx2 oxidation is through non-reducing gel-electrophoresis followed by western blot for Prx2 monomers and dimers21. While this approach has proven useful in many studies, it requires laborious gel electrophoresis procedures, pooled cell lysate samples, and often results in a large amount of variability from sample to sample. For ultrasensitive H2O2 detection, several groups have designed genetically-encoded fluorescent probes for H2O2-induced peroxiredoxin oxidation; this approach has yielded success in yeast22, where the designed probe was more sensitive than the current genetically-encoded sensors HyPer and roGFP2-Orp1, as well as plants23,24,25, but has not been applied to human cells, where HyPer and roGFP2-Orp1 are state-of-the-art tools. In this work, we created a fluorescence resonance energy transfer (FRET) probe based upon the reactivity of human Prx2 for ultrasensitive detection of H2O2 in human cells. In order to maximize the fluorescence change in the two fluorophores upon dimerization of the reactive domain of the probe, we chose the fluorescent proteins Clover and mRuby2 as a FRET pair, which were previously shown to have a higher dynamic range than conventional cerulean-yellow fluorescent protein FRET pairs and insensitivity to changes in pH26. In order to validate this new tool, we purified the fluorescent probe and quantified its fluorescent signal in response to various oxidants and buffers in order to determine its selectivity and pH sensitivity. Additionally, we expressed the probe in human epithelial cells and quantified its fluorescent signal in response to various perturbations chosen to assess sensitivity towards H2O2. Following these detailed characterization and validation studies, we were able to detect and track low levels of H2O2-induced Prx2 oxidation when malignant cells were treated with the cancer therapeutics auranofin and piperlongumine. Even though the small elevation of H2O2 in response to piperlongumine treatment was sufficient to cause apoptosis14, the H2O2 level was below the lower detection limit of HyPer, which highlights the unique ability of the probe developed in this work to detect small, yet relevant, changes in intracellular H2O2 levels using a responsive domain that is of broad interest in many biological processes27,28,29.
Probe responds rapidly and robustly to H2O2
In order to construct an ultrasensitive H2O2 probe, the genes corresponding to the three domains of the probe (Clover, Prx2, mRuby2) were inserted into pcDNA3.1+ via the Gibson assembly method. Additionally, between each fluorescent protein and the central Prx2 domain, a short flexible amino acid linker was inserted to promote proper folding of the fluorescent protein domains (Fig. 1a). In order to characterize the response of the cytosolic probe to H2O2, a culture of HeLa cells was transfected with the gene encoding the probe, exposed to an external bolus addition of H2O2, and imaged with a widefield microscope. Upon addition of H2O2 to the culture, the intensity in the donor fluorescence channel decreased while the intensity in the donor–acceptor FRET fluorescence channel increased (Fig. 1b, c). These changes resulted in a rapid increase in the emission ratio of the probe, defined as the ratio of intensity in the FRET fluorescence channel divided by the intensity in the donor fluorescence channel (Fig. 1d). Upon mutation of both the catalytic and resolving cysteine residues of the Prx2 domain of the probe to serine, addition of H2O2 did not elicit a change in the emission ratio, suggesting that the observed change in the signal of the unmodified probe depends on the activity of one, or both, of these cysteines (Fig. 1e). In contrast, mutation of only the catalytic cysteine of the probe followed by addition of H2O2 resulted in a slight increase in the emission ratio of the probe, but not as large as the original construct (Supplementary Fig. 1a), which suggests that disulfide bond linkages may also form between probe molecules and unmodified, endogenous Prx2 monomers that result in slight conformational changes in the probe. Interestingly, mutation of only the resolving cysteine of the probe followed by addition of H2O2 slightly decreased the emission ratio of the probe relative to the pre-stimulation value (Supplementary Fig. 1b), which may reflect hyperoxidation of the catalytic cysteine of the probe from a sulfenic acid to a sulfinic or sulfonic acid30. We present the results of these studies in terms of moles of H2O2 per cell as opposed to concentration or total moles of H2O2. As we and others have previously shown31,32,33,34, both the total moles of H2O2 added as well as the total number of cells present affect the results obtained from this kind of experiment, whereas moles of H2O2 per cell is a meaningful metric that may be compared across studies.
As a complement to intracellular measurements, we purified the fluorescent probe in order to assess its specificity to H2O2 and sensitivity to environmental factors such as pH. In order to purify the fluorescent protein, an N-terminal histidine-tagged construct was expressed in bacteria, and the recombinant protein was purified via immobilized metal affinity chromatography (Supplementary Fig. 2). The purified protein was subjected to various oxidants to determine the specificity of the probe (Supplementary Fig. 3 and Supplementary Fig. 4). Of the oxidants tested, only H2O2 and tert-butyl hydroperoxide (TBHP) significantly increased the emission ratio of the fluorescent protein, consistent with previous measurements with fluorescent peroxiredoxin-based constructs22. In addition to these experiments, the probe was also subjected to two different treatments to determine the sensitivity of peroxynitrite; in the first experiment, purified protein was exposed to the peroxynitrite generator compound linsidomine (SIN-1), whereas in the second, HeLa cells expressing the probe were exposed to a solution with nitric oxide, which diffuses into cells and rapidly reacts with superoxide to form peroxynitrite (Supplementary Fig. 5 and Supplementary Fig. 6). In both instances, the signal from the probe did not increase significantly after the treatment, once again consistent with previous measurements with analogous constructs22. In a separate set of experiments, we also tested the robustness of the purified probe to buffers with different pHs (Supplementary Fig. 7). The results of this study indicate that the emission ratio of the both of the reduced and oxidized probe remain relatively constant within the pH range of 6.6 to 7.8.
Probe responds to low levels of extracellular H2O2
We further assessed the dynamics of probe oxidation and reduction with several additional H2O2 bolus perturbations. We first determined the amount of H2O2 required to completely saturate the probe (Fig. 2a), and then titrated down with lower quantities of H2O2 in order to study the dynamic behavior of the system (Fig. 2b-g, Supplementary Fig. 8). In order to test whether the probe responded to successive additions of H2O2, we also stimulated cells expressing the probe with a H2O2 bolus equivalent to that in Fig. 2c, waited until the emission ratio returned to the baseline level, and added a second H2O2 bolus of the same amount (Supplementary Fig. 9). In addition, in order to determine how long the probe signal persisted in the cell after saturation of the probe, we stimulated cells expressing the probe with a H2O2 bolus equivalent to that in Fig. 2a and waited until the emission ratio returned to the baseline level (Supplementary Fig. 10). Lastly, in order to determine the minimum and maximum fold changes for the cytosolic probe, cells with the probe were exposed to the thiol oxidant diamide and the disulfide reductase dithiothreitol, and the final fold change of the probe signal over the 10-min measurement period was calculated (Supplementary Fig. 11). As expected, maximum fold change achieved with diamide was higher than that achieved with H2O2 bolus addition, as diamide likely oxidizes intracellular reductases such as Trx and thus prevents the reduction of oxidized Prx protein. Additionally, the sensor fold change did not change upon addition of dithiothreitol to the cell culture, which suggests that the probe exists almost entirely in the reduced form at basal levels of intracellular H2O2. This finding agrees well with results from kinetic models of this system, which predict that approximately 99.8% of Prx2 resides in the reduced state at basal H2O2 concentrations16.
To examine whether cytosolic expression of the probe perturbs the native, H2O2-clearing activity of endogenous Prx2, we stimulated cells with and without the fluorescent probe with various amounts of external H2O2, subjected the lysates from these cells to non-reducing, denaturing gel electrophoresis (i.e., non-reducing SDS-PAGE) followed by immunoblots for Prx2, and quantified the fraction of dimerized Prx2 in each sample (Fig. 3a–c). These membranes were also blotted for hyperoxidized Prx in order to ensure that no hyperoxidized species formed during these experiments (Supplementary Fig. 12). In lysates from these cells, two clear protein bands appeared that corresponded to reduced Prx2 monomers (21 kDa) and oxidized dimers (42 kDa) in the cytosol. Comparison of Fig. 3a with 3b indicates that expression of the probe does not shift the onset of native Prx2 dimerization.
More detailed examination of these redox western blots, provided in full as Supplementary Fig. 13, revealed several higher-molecular weight protein bands that likely correspond to the reduced probe monomers (75 kDa), oxidized heterodimers between probe and Prx2 monomers (100 kDa) and oxidized homodimers between two identical probe molecules (150 kDa). In order to observe the native state of the probe, lysates from cells expressing the probe were subjected to blue-native gel electrophoresis (BN-PAGE) and immunoblotted for both Prx2 and green fluorescent protein (Supplementary Fig. 14). No bands greater than 150 kDa were detected in the native blots, which suggests that the probe molecules do not assemble into high-molecular weight oligomers in these cells under the conditions used in our experiments.
Probe responds to low levels of intracellular H2O2
To progress beyond convenient, yet unrealistic, bolus additions of H2O2 we used the enzyme D-amino acid oxidase (DAAO), which has been previously used to produce continuous, tunable amounts of H2O2 within mammalian cells35,36,37. We expressed this enzyme in HeLa cells that contained either the Prx2-based probe or HyPer, a genetically-encoded H2O2 probed based upon the bacterial transcription factor OxyR, which is known to react with H2O2 with a second order rate constant nearly two orders of magnitude slower than Prx238. Upon expression of DAAO in cell lines with the fluorescent probes, we supplied cells with various amounts of D-alanine, a substrate of DAAO, which resulted in steady production of H2O2 over a 4.5-h time period (Fig. 4a and Supplementary Fig. 15). We then measured the fluorescent signal from the two probes in each cell line over the same time period, and compared the initial signal with the final signal for each concentration of D-alanine (Fig. 4b, c and Supplementary Fig. 16). In these experiments, the signal from the Prx2-based probe increased significantly compared to baseline value even at the lowest concentration of D-alanine concentrations, whereas the HyPer signal did not increase significantly compared to the baseline value at D-alanine concentrations below 25 mM. In addition to this result, we observed that the signal from the Prx2-based probe appeared to saturate at D-alanine concentrations above 15 mM, whereas the HyPer signal gradually increased as the D-alanine concentration increased. Together, these results suggest that the Prx2-based probe responds to lower intracellular amounts of H2O2 compared to HyPer.
Probe responds to decreases in TrxR activity
We next sought to determine the sensitivity of the Prx2-based probe to changes in Trx/TrxR activity. Since endogenous Prx2 utilizes the Trx/TrxR system for reduction of oxidized protein, we hypothesized that the Trx/TrxR system would also reduce probe molecules, and that inhibition of this reduction machinery would result in rapid accumulation of oxidized probe molecules coupled with an increased emission ratio from the probe. In order to test this hypothesis, we treated cells expressing either the Prx2-based probe or HyPer with the known TrxR inhibitor and cancer therapeutic compound auranofin8,11, which has been previously shown to cause the accumulation of oxidized Prx2 dimers in human cells21 (Fig. 5a). Since HyPer primarily utilizes glutaredoxin for reduction of oxidized protein39, we expected that this probe would be less sensitive to auranofin induced perturbations, and would only respond after significant accumulation of oxidized Prx2, which would allow HyPer to compete more effectively for intracellular H2O2. As expected, when HeLa cells were treated with auranofin, the activity of TrxR in the lysate decreased in a dose-dependent manner (Fig. 5b and Supplementary Fig. 18). Concomitant with this decrease in TrxR activity, the signal from the Prx2-based probe increased significantly compared to the baseline value at inhibitor concentrations above 1 μM, and quickly saturated at higher concentrations (Fig. 5c). In contrast, the HyPer signal only increased significantly from the baseline value at concentrations at or above 16 μM, and continued to increase as the inhibitor concentration increased (Fig. 5d). This change also corresponded to an increase in the intensity of Prx2 dimers as well as probe-based dimers, which started to accumulate on western blots at inhibitor concentrations above 8 μM (Supplementary Fig. 19). Together, these observations suggest that the Prx2-based probe rapidly responds to changes in the activity of the Trx/TrxR system, and support the hypothesis that HyPer, which primarily utilizes glutaredoxin for reduction, only responds significantly to the treatment after accumulation of oxidized Prx2 occurs.
Probe responds to therapeutic-induced H2O2 elevation
In our last set of experiments, we asked if we could use the Prx2-based probe to detect redox perturbations associated with the redox therapeutic piperlongumine13. Recently, Huang et al.14. demonstrated that treatment of human epithelial cells with 10 μM piperlongumine results in the elevation of oxidized Prx2 dimers in addition to irreversible S-glutathionylation of proteins and cell death14. The authors of this study also showed that upon over-expression of catalase in the cytosol of these cells, piperlongumine treatment resulted in significantly less oxidized Prx2 in addition to lower cytotoxicity, which suggests that elevation of cytosolic H2O2 is central to the toxicity of piperlongumine. In order to assay for increased intracellular H2O2 after the addition of piperlongumine, we incubated a culture of HeLa cells with the drug for a period of 10 h and immuno-blotted the lysates for Prx2. Treatment of these cells with a dose of piperlongumine that was shown in past work13 to selectively kill several kinds of cancer cells caused an observable increase in the level of Prx2 dimerization compared to the control case (Fig. 6a). We next stably expressed the Prx2-based probe in the cytosol of HeLa cells and recorded the emission ratio of the sensor in the cells at various points in time after addition of piperlongumine (Fig. 6b). Upon addition of the drug to the cell culture media, the emission ratio of the probe began to increase after approximately 4–6 h, and steadily increased for the remainder of the 10-h incubation period (Fig. 6c, d). This signal was also reversible upon introduction of additional catalase in the cytosol (Supplementary Fig. 20). We then stably expressed the Prx2-based probe in the cytosol of A549 cells, which are known to overexpress certain antioxidant proteins as a result of hyperactive nuclear factor erythroid 2-related factor 2 (Nrf2)40,41, and repeated the previous experiment. As expected, upon stimulation with the drug, the signal from the probe did not increase, suggesting a lower cytosolic H2O2 concentration in response to the drug (Fig. 6e, f). In our last set of experiments, we stably expressed the H2O2 probe HyPer in the cytoplasm of HeLa cells and performed the piperlongumine experiment one more time. Upon stimulation with the drug, the HyPer emission ratio did not significantly change from the control case over the 10-h incubation period (Fig. 6g, h). These results agree with the results obtained by Huang et al.14, and suggest that the cytoplasmic H2O2 flux required for this Prx2 oxidation is below the detection limit of HyPer.
Due to the variety of roles that different oxidants play in both healthy and diseased cells, there is significant interest in the development of new fluorescent probes that can isolate the effects of therapeutic agents that perturb these species. Synthetic small molecule fluorescent probes for H2O2 have been developed, but many of these probes lack specificity and/or sensitivity for H2O242,43,44,45. Similarly, several protein-based fluorescent probes that utilize the H2O2-sensitive protein domains of OxyR and Orp1 have been reported and have been successfully applied to study the effects of several H2O2-mediated processes in human cells38,39. Because Prxs react with H2O2 much more rapidly than these probes, some processes that only slightly elevate H2O2 may require a more highly reactive probe38. Recently, Morgan et al.22. designed a fluorescent probe based upon the redox relay between the yeast peroxiredoxin Tsa2 and the fluorophore roGFP2 and applied it in yeast, where it was more sensitive than other probes based on less reactive peroxidase domains22. In this study, we designed a probe based on human Prx2, the dominant peroxiredoxin species in human cells; this design allowed us to accurately track small increases in intracellular H2O2 that could not be detected using state-of-the art genetically-encoded probes.
Based on our choice of Prx2 as the reactive domain of the probe, we expected that the fluorescent probe would react with a similar specificity towards H2O2 and in a similar responsive range as the endogenous protein. Indeed, our experiments with the purified probe protein suggest that the probe has very high selectivity for H2O2 over other oxidants, with the exception of TBHP, which also oxidizes endogenous Prxs. In agreement with previous measurements from peroxiredoxin-based probes, TBHP caused an increase in the signal from our probe, but the other oxidants tested did not. The emerging literature related to ferroptosis46,47 has drawn new attention to the intersection of iron homeostasis and dysregulation with intracellular levels of various peroxides. Use of the Prx probe we report to study alterations in iron homeostasis will warrant additional validation experiments to understand whether and how such differences influence the signal from the probe.
Our experiments with different pH buffers suggest that the Prx FRET probe has better pH stability than HyPer, as a result of the choice of fluorophores rather than the choice of H2O2 reactive domains. As originally reported, HyPer’s excitation ratio (500 nm/420 nm) in the reduced form at pH 7.4 is greater than its excitation ratio in the fully oxidized form at pH 7.2, and pH controls are always necessary. Supplementary Fig. 7 shows that the Prx probe’s emission ratios in the reduced form are always less than the emission ratios exhibited by the oxidized form. The magnitude of the error bars in Supplementary Fig. 7 demonstrate that pH controls should only be necessary in the subset of experiments that show small FRET increases comparable with variability in the values for the reduced probe as a function of pH.
Our measurements from the cytosolic probe upon stimulation with different amounts of H2O2 indicate that the fluorescent Prx2-based probe has a responsive range similar to that of the native, unmodified protein. Reactivity with H2O2 does not appear to be altered by fusion with two fluorescent protein domains. Moreover, the relatively low cell-to-cell variability of the probe’s signal (approximately 5% of the maximum fold change in these experiments) allowed us to visualize very small differences in external H2O2. Based on bolus addition experiments, the probe reached saturation upon exposure to approximately 30 fmol H2O2 per cell. Given that peroxiredoxin does not become hyperoxidized21 at this concentration (Supplementary Fig. 12) the gradient of H2O2 across the plasma membrane of HeLa cells is approximately 650-fold48, and this extracellular concentration corresponds to an intracellular concentration of approximately 15 nM. If the H2O2 perturbation in a process of interest is extreme enough that significant hyperoxidation or saturation of oxidized Prx2 would result, an ultrasensitive probe such as the one we report here would not be needed, and other H2O2 probes, such as HyPer or roGFP2-Orp1, may serve as the better choice. We advocate experiments using complementary techniques such as immunoblots with antibodies specific to hyperoxidized Prx in cases where extreme intracellular levels of H2O2 are suspected, or to rule these out.
Similar to other genetically-encoded fluorescent probes for H2O2, we observed a slight shift in the fluorescence ratio of the probe before addition of any oxidant in experiments occurring on the minutes-timescale where samples were imaged frequently. In Fig. 2 and in Supplementary Figures 9 and 10, a slight upward drift of the emission ratio is apparent. Examination of the data used to calculate emission ratios shows that the upward drift is a true FRET signal rather than a ratio change attributable to photobleaching. This monotonic upward drift was not observed in experiments that occurred on the hours-timescale with less frequent imaging (Fig. 6, Supplementary Figure 16). In order to aid in interpretation of data in all of these experiments, we recommend the use of control experiments in which media without any stimulant is added to cells so that changes in the emission ratio of the cells after addition of the stimulant can be compared with the baseline signal over the same period of time.
Given that the concentration of endogenous Prxs is on the order of 1 × 10−4 M16,18, and that the typical concentration of fluorescent protein constructs is on the order of 1 × 10−6 M49, we hypothesized that the presence of the probe would not significantly affect the level of oxidized Prx2 in the cytosol upon stimulation with H2O2. As expected, the concentration of the intracellular probe was determined to be approximately 1 × 10−6 M (Supplementary Fig. 21), and expression of the probe in the cytosol did not significantly affect the observed amount of oxidized endogenous Prx2 dimers upon treatment with H2O2, although we anticipate that much higher expression levels of the probe may impact the activity of the endogenous protein. Currently, it is not known whether recombinant peroxiredoxin constructs assemble into high-molecular weight oligomers upon expression in mammalian cells50 in a similar fashion to analogous probes in yeast22. While we did see evidence of high molecular weight oligomers of endogenous Prx2 in our native gel electrophoresis experiments in samples probed with an antibody specific to human Prx2 (in agreement with previous studies conducted with lysates from mammalian cells51,52), we did not see any significant formation of high molecular weight oligomers in samples probed with antibody against GFP, which suggests that the probe does not form these oligomeric complexes under the conditions we used and does not require them to function in these cells.
The probe developed in this work was also characterized in terms of sensitivity towards intracellular H2O2 generation. Most current genetically-encoded H2O2 sensors (e.g., HyPer and roGFP2-Orp1) are based on protein domains that react with H2O2 with a relatively low rate coefficient (on the order of 1 × 105 M−1s−1)38. In contrast, Prx2 reacts with H2O2 with a rate coefficient nearly two orders of magnitude higher (on the order of 1 × 107 M−1s−1)17. In combination with the abundance of Prx2 in the cytosol of human cells (on the order of 1 × 10−4M)16,18 and reduction by Trx, Prx2 kinetically outcompetes other potential reaction partners for H2O2 in the cell, which allows it to respond to very small fluctuations in the H2O2 level before other proteins that contain less reactive cysteines can react with H2O2. In our experiments with the cytosolic H2O2 generator DAAO, cells expressing the Prx2-based probe responded to lower amounts of the D-alanine compared to cells expressing HyPer over the timescale of hours (Supplementary Fig. 16) as well as immediately after D-alanine addition (Supplementary Fig. 17). We observed that higher concentrations of D-alanine were required to induce changes in HyPer’s ratiometric signal in comparison with a previous study37. This difference may result from differences in the expression levels of DAAO or HyPer or both; each are expected to impact the results37,49. In order to control for variability expected as a function of DAAO’s expression level, we transfected cell lines with equal amounts of DAAO in order to compare the two fluorescent probes. Our results from this experiment suggest that Prx2-based probe responds to lower levels of intracellular H2O2 than HyPer, and based on the results obtained from Morgan et al.22., we expect that this probe would also out-compete other genetically-encoded probes for H2O2 with similar reactivity as HyPer.
After detailed characterization of the probe’s reactivity with H2O2, we used it to detect perturbations caused by redox cancer therapeutics. Recently, several studies have suggested that Prxs may serve as the focal point for many redox-based stress response pathways due to their ability to not only rapidly clear H2O2 from the cytosol, but also to directly affect the activity of other redox-regulated proteins in the cytosol through disulfide exchange mechanisms53,54. Cells utilize reductase enzymes such as Trx and TrxR to maintain a large pool of reduced Prxs in the cytoplasm which can respond to small fluctuations in H2O2. One way redox cancer therapeutics are thought to perturb this Prx/Trx/TrxR pathway in cancer cells is through inhibition of the reduction pathways that cells used to regenerate reduced antioxidant enzymes, which results in accumulation of oxidized peroxiredoxin species that can participate in signaling reactions9,11. In our experiments with the TrxR inhibitor auranofin, we treated cancer cells with an amount of inhibitor sufficient to cause near-complete inhibition of TrxR activity and observed a significant increase in the emission ratio of our probe. Other redox therapeutics also cause an increase in the intracellular level of H2O2 that results in selective toxicity to a wide variety of tumors12. In our experiments with the compound piperlongumine, we treated cancer cells with an amount of drug previously shown to induce elevation of cytosolic H2O2 that was essential to toxicity over a 48 h period14 and observed a significant increase the emission ratio of our probe, which was reversible upon over-expression of catalase in the cytosol. In both of the above examples, we were unable to see any change in the emission ratio from HyPer, which suggests that the probe developed in this work has the capability to sense these slight, but physiologically-relevant, redox therapeutic-induced perturbations.
In conclusion, we created and characterized an ultrasensitive genetically-encoded probe for H2O2 based on human Prx2. We used this probe to track changes in TrxR activity as well as intracellular H2O2 generation in response to the cancer therapeutics auranofin and piperlongumine, respectively. We also compared the signal of this probe to that of another commonly used probe in the field, which was unable to detect the slight perturbations associated with these treatments. We believe that these capabilities highlight the potential of this probe as a valuable tool for the measurement of H2O2-mediated processes in human cells, and uniquely position it to offer insight into the mechanism of action a number of different therapeutic compounds in which elevation of oxidative species is thought to play a role8,9,11,55.
All reagents were obtained from Sigma-Aldrich unless otherwise specified.
Construction of fluorescent probe
The cloning sequences for Clover, mRuby2, and Prx2 were isolated from their original plasmids via polymerase chain reaction (PCR). pcDNA3.1-Clover-mRuby2 was a gift from Kurt Beam (Addgene plasmid # 49089); pQTEV- PRDX2 was a gift from Konrad Buessow (Addgene plasmid # 31338). Next, splice overlap extension PCR reactions were used to add 30-40 nucleotide sequences to the 3’ of the Clover gene and the 5’ end of the mRuby2 gene. These oligonucleotides contained the sequences for the linker sequences between the fluorescent protein and Prx2 domain and also an overlap region between the fluorophore and the Prx2 domain for the Gibson assembly reaction. Next, a second round of splice overlap extension PCR reactions were used to add vector overlap sequences to the 5’ end of the Clover gene and the 3’ end of the mRuby2 gene. In order to create the final fusion product, the three gene fragments were assembled into a linearized vector via Gibson assembly. In order to obtain the linearized vector for the Gibson assembly reaction, the pcDNA3.1(+) mammalian expression vector (ThermoFisher Scientific) was digested with EcoRI/XhoI (New England Biolabs) for 15 min at 37 °C. The three gene fragments were then co-incubated with the linearized vector and Gibson assembly master mix (New England Biolabs) at 60 °C for 60 min.
Cell culture and stable cytosolic expression of probe
Hela cells (ATCC® CCL-2) and HEK-293 cells (ATCC® CRL-1573) lines were cultured in Dulbecco’s Modified Essential Medium (DMEM; Lonza) with 10% Fetal Bovine Serum (FBS; ATCC) at 37 °C, 95% air and 5% CO2. Cell lines obtained directly from ATCC were not authenticated or tested for mycoplasma. Media in the culture dish was replaced with fresh media every 2–3 days, and the cells were passaged every 4–5 days. In order to create a cell line with stable expression of the fusion construct, the fluorescent protein was constitutively expressed under control of the human cytomegalovirus (CMV) promoter with the pLJM1 lentiviral vector; the original plasmid, pLJM1-EGFP, was a gift from David Sabatini (Addgene plasmid # 19319). A HEK 293FT cell culture was first transfected with the plasmid of interest along with lipofectamine 2000 (Invitrogen) in Opti-MEM media (ThermoFisher Scientific) via the manufacturer’s instructions. 24 h after transfection, the media in the HEK culture was replaced with DMEM supplemented with 10% FBS; after an additional 24 h of incubation, the media in the HEK culture was collected and the supernatant containing the lentiviral particles was isolated. The lentiviral particles were added to a separate HeLa cell culture along with 6 μg/mL polybrene and the cells were incubated for approximately 72 h. The HeLa cell were then removed from the dish with 0.25% Trypsin-EDTA (ThermoFisher Scientific) and re-plated onto a 10 cm dish with 8 mL of DMEM supplemented with 10% FBS and 6 μg mL−1 puromycin. Cells were grown in this selective media for a period of 4-5 days before the cell stocks were prepared.
Characterization of probe reactivity in human cells
Prior to the experiment, either 1.75 × 105 cells or 3.5 × 105 cells were seeded into a single well of a 6-well plate (Corning) with approximately 9.5 cm2 of available growth area. After either 24 h or 48 h of incubation, respectively, cells were transiently transfected with 1.5 μg of the plasmid of interest with Lipofectamine 2000 according to the manufacturer’s instructions. After 24 h of incubation, cells were washed once with 1 mL of phosphate buffered saline (PBS) (1×) and then placed in 1 mL of Roswell Park Memorial Institute (RPMI) 1640 media with no phenol red (ThermoFisher Scientific). For each data set, baseline signal was acquired for 2.5 min; once 2.5 min had elapsed, 1 mL of RPMI 1640 medium with 40 μM H2O2 was added to the cells in each well to reach a final concentration of 20 μM H2O2 in a total volume of 2 mL a final cell density of 3.5 × 108 cells L−1. Approximate values for mols H2O2 per cell were calculated by dividing the concentration of H2O2 (in units of mols L−1) by the cell density (expressed in cells L−1).
Characterization of cytosolic probe dose response to H2O2
Prior to the experiment, either 1.75 × 105 cells or 3.5 × 105 cells stably expressing the fluorescent Prx2 probe were seeded onto each well of a 6-well plate with approximately 9.5 cm2 of available growth area. The day of the experiment, cells were washed with 1 mL of PBS (1×) and then placed in 1 mL of RPMI 1640 media with no phenol red. For each data set, baseline signal was acquired for 2.5 min. After 2.5 min had elapsed, 1 mL of RPMI 1640 medium with an H2O2 concentration of 40, 20, 10, 2, or 0 μM H2O2 was added to the cell culture to reach final H2O2 concentrations of 20, 10, 5, 1, and 0 μM H2O2, in a total volume of 2 mL along with a final cell density of 3.5 × 108 cells L−1. Fluorescent images were acquired in the same manner as described as above. As before, approximate values for the mol H2O2 per cell were calculated by dividing the concentration of H2O2 (in units of mol L−1) by the cell density (expressed in cells L−1).
Production of intracellular H2O2 from D-amino acid oxidase (DAAO)
In order to continuously produce H2O2 in the cytosol of HeLa cells, DAAO, which we previously14 cloned into the pLJM1-EGFP construct (Addgene plasmid # 19319), was stably expressed under control of the CMV promoter in the cytosol of HeLa cells containing either the Prx2-based probe or HyPer with the procedure described above. Cells were grown in the selective media for a period of 2 days before the cells were passaged again. The day before the experiment, 3.5 × 105 cells were seeded onto the surface of a 6-well plate. The day of the experiment, cells were washed once with PBS (1×) and supplemented with 1 mL of RPMI 1640 (1×) containing 5 μM flavin adenine dinucleotide (FAD). Before addition of D-alanine (the DAAO substrate) to the wells, images in each fluorescent channel were acquired for each well. After these images were acquired, D-alanine (dissolved in RPMI 1640) was added to the wells to reach final D-alanine concentrations of 50, 25, 15, and 7.5 mM in a total volume 2 mL; as a control, an equivalent volume of RPMI 1640 with no D-alanine was added to a separate cell sample. Images were acquired in each fluorescent channel in each well after 1, 2, and 4.5 h.
Treatment with auranofin and piperlongumine
For experiments that involved the TrxR inhibitor auranofin (Cayman Chemical Company), solid auranofin was dissolved in sterile-filtered DMSO to make a 32 mM stock solution the day of the experiment. The auranofin stock solution was then added to culture medium comprised of DMEM supplemented with 10% FBS to reach final auranofin concentrations of 32, 16, 8, 4, and 1 μM in a total volume of 2 mL culture medium. Pure dimethyl sulfoxide (DMSO) was used as a control. Cells were then incubated at 37 °C for a period of 90 min. After incubation, the signal in each fluorescent channel was acquired in three different regions of each well. For experiments that involved the cancer therapeutic piperlongumine, solid piperlongumine (Cayman Chemical Company) was dissolved in DMSO to make 30 mM stock solution the day of the experiment. Before addition of the piperlongumine to the cell culture, images in each fluorescent channel were acquired for each well. After these images were acquired, the piperlongumine stock solution was then added to the media to reach a final piperlongumine concentration of 10 μM in a total volume 2.5 mL; as a control, an equivalent volume of DMSO was added to a separate cell sample. Images were acquired in each fluorescent channel in each well every 2 h for a period of 10 h.
Selenocystine assay for TrxR activity
Immediately after treatment with auranofin, cells were washed twice with PBS (1×) on ice then treated with 100 µL of 1% Triton x-100 supplemented with 1x HALT protease cocktail (ThermoFisher Scientific) to lyse the cells. The lysed cells were then re-pelleted at 12,000 g for 10 min. The supernatant of the solutions was then collected and the protein content in the solution was assessed via the bicinchoninic acid (BCA) assay. 40 μg of protein were then added to a solution containing 400 μM NADPH (VWR) and 800 μM selenocystine (ThermoFisher Scientific). The absorbance of the solution at 340 nm was then measured every 2 min for a period of 90 min.
Gel electrophoresis and peroxiredoxin-2 western blots
For each experiment in which western blots were used, the adherent cells were first washed twice with PBS (1×). For experiments in which H2O2 was used, the liquid in each well was replaced with 1 mL of PBS and the H2O2 was added to the wells in the same manner as above. After approximately 30 s, the liquid in each well was aspirated and replaced with 2 mL of 100 mM methyl methanethiolsulfonate (MMTS) in order to block any free thiol groups as described previously. The plates were then incubated on ice for a period of 20-30 min, and washed twice with 1 mL PBS. Next, 100 µL of 1% Triton x-100 supplemented with 1x HALT protease cocktail was added to lyse the cells. The lysed cells were then re-pelleted at 12,000 g for 10 min. The supernatant of the solutions was then collected and the protein content in the solution was assessed via the BCA assay. 25 μg of protein was then loading into a tris-tricine acrylamide gel and subjected to SDS-PAGE under non-reducing conditions (i.e. with no β-mercaptoethanol in sample buffer). After the separated lysates were transferred to a PVDF membrane (Bio-Rad) (1 h at 100V), the blot was blocked and incubated with either goat primary antibody for Prx2 (R&D Systems, Catalog # AF3489) (at a dilution of 1:800) or goat primary antibody for GFP (R&D Systems, Catalog # AF4240) at 4 °C overnight and then with donkey anti-goat IR 688 (Licor) (at a dilution of 1:10,000) for 1 h at approximately 22 °C. The tagged proteins were then visualized on the Odyssey CLx Infrared Imaging System. GAPDH was used as a loading control for all blots. Uncropped version of all blots in main text shown in Supplementary Figure 22.
Fluorescent measurements and image analysis
Adherent cells were imaged with an inverted Olympus IX81 widefield microscope with an Olympus plan fluorite 10x dry objective (NA = 0.30). A 200 W metal-arc lamp (Prior) was used as a power source. For ratiometric FRET measurements, two different filter sets were used: for Clover fluorescence measurements, a 488/6 nm excitation filter (Semrock) was used with a 525/40 nm emission filter (Semrock); for Clover-mRuby2 FRET fluorescence measurements, a 488/6 nm excitation filter was used with a 625/40 nm emission filter (Semrock). A 1000 ms exposure time at 25% lamp intensity was used to acquire images in both fluorescent channels. For image analysis, 16-bit.tif images were exported to ImageJ and regions-of-interest (ROIs) were then drawn around cells to be analyzed. In order to calculate the raw emission ratio in each ROI at each time point, the background-subtracted FRET fluorescence intensity was divided by the background-subtracted Clover fluorescence intensity in ImageJ.
For experiments in which the fluorescent probe HyPer was used, the following two filter sets were used: for short wavelength HyPer excitation, a 415/6 nm excitation filter (Semrock) with a 525/40 nm emission filter (Semrock); for long wavelength HyPer excitation, a 488/6 nm excitation filter with a 525/40 nm emission filter (Semrock). A 250 ms exposure time at 10% lamp intensity was used to acquire the images in both channels. ROIs were determined in images from both fluorescent channels as above. In order to calculate the ratiometric HyPer signal (i.e., the HyPer ratio) in each ROI at each time point, the background-subtracted fluorescence intensity from the 488 nm excitation channel was divided by the background-subtracted fluorescence intensity from the 415 nm excitation channel in ImageJ.
For all images, background-subtraction was performed in ImageJ with the rolling ball algorithm. In order to create the emission ratio image, the background-subtracted 16-bit.tif images for each fluorescent channel were imported into MATLAB and divided pixel-by-pixel.
All experimental emission ratio values are reported as the mean value ± S.E.M. In order to calculate the mean raw emission ratio value for each sample, the raw emission ratio was calculated for the designated number of cells from the background-subtracted images for each timepoint; these values were then averaged to obtain the raw emission ratio values for these cells. Neither the brightest cells nor the dimmest cells were used for probe characterization. Probe fold change values are reported as mean value ± S.E.M.; mean probe fold change values were determined in a similar manner to the raw emission ratios. The normalized fold change values were determined from the average fold change values from the control and experimental samples. The error associated with these average ratios was propagated according to the following expression:
where σNFC, σFC, and σFC(control) are the uncertainties in the normalized fold change, the peak fold change value, and the peak fold change value of the control sample (i.e., 0 μM H2O2), respectively. NFC, FC, and FC (control) are the normalized fold change value, the peak fold change value, and the peak fold change value of the control sample, respectively.
For the piperlongumine experiment, the final probe fold change values were determined from the average raw emission ratios at the final and initial time points. The error associated with these average ratios was propagated according to the following expression:
where σFC, σR(t=0), and σR(t=10) are the uncertainties in the fold change, the raw emission ratio at 0 h, and the raw emission ratio at 10 h, respectively. FC, R(t = 0), and R(t = 10) are the fold change value, the raw emission ratio value at 0 h, and the raw emission ratio value at 10 h, respectively. In order to assess the significance of differences in the probe’s fold-change values in all experiments, a two-tailed student’s t-test was used at a significance level of p = 0.02.
The authors declare that the data supporting the findings of this study are available within the paper and its supplementary information files. All constructs created in this study are available upon request.
Holmström, K. M. & Finkel, T. Cellular mechanisms and physiological consequences of redox-dependent signalling. Nat. Rev. Mol. Cell Biol. 15, 411–421 (2014).
Winterbourn, C. C. Reconciling the chemistry and biology of reactive oxygen species. Nat. Chem. Biol. 4, 278–286 (2008).
Rhee, S. G. Redox signaling: hydrogen peroxide as intracellular messenger. Exp. Mol. Med. 31, 53–59 (1999).
Rhee, S. G. et al. Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Curr. Opin. Cell Biol. 17, 183–189 (2005).
Zhang, C. et al. Calyxin Y induces hydrogen peroxide-dependent autophagy and apoptosis via JNK activation in human non-small cell lung cancer NCI-H460 cells. Cancer Lett. 340, 51–62 (2013).
Cairns, R. A., Harris, I. S. & Mak, T. W. Regulation of cancer cell metabolism. Nat. Rev. Cancer 11, 85–95 (2011).
Nogueira, V. & Hay, N. Molecular pathways: reactive oxygen species homeostasis in cancer cells and implications for cancer therapy. Clin. Cancer Res. 19, 4309–4314 (2013).
Gorrini, C., Harris, I. S. & Mak, T. W. Modulation of oxidative stress as an anticancer strategy. Nat. Rev. Drug Discov. 12, 931–947 (2013).
Trachootham, D., Alexandre, J. & Huang, P. Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat. Rev. Drug Discov. 8, 579–591 (2009).
Dobbelstein, M. & Moll, U. Targeting tumour-supportive cellular machineries in anticancer drug development. Nat. Rev. Drug Discov. 13, 179–196 (2014).
Wondrak, G. T. Redox-directed cancer therapeutics: molecular mechanisms and opportunities. Antioxid. Redox Signal. 11, 3013–3069 (2009).
Chaiswing, L., St Clair, W. H. & St. Clair, D. K. Redox Paradox: a novel approach to therapeutics-resistant cancer. Antioxid. Redox Signal. (2018). https://doi.org/10.1089/ars.2017.7485
Raj, L. et al. Selective killing of cancer cells by a small molecule targeting the stress response to ROS. Nature 475, 231–234 (2011).
Huang, B. K., Langford, T. F. & Sikes, H. D. Using sensors and generators of H2O2 to elucidate the toxicity mechanism of piperlongumine and phenethyl isothiocyanate. Antioxid. Redox Signal. 24, 1–52 (2016).
Adimora, N. J., Jones, D. P. & Kemp, M. L. A model of redox kinetics implicates the thiol proteome in cellular hydrogen peroxide responses. Antioxid. Redox Signal. 13, 731–743 (2010).
Lim, J. B., Huang, B. K., Deen, W. M. & Sikes, H. D. Analysis of the lifetime and spatial localization of hydrogen peroxide generated in the cytosol using a reduced kinetic model. Free Radic. Biol. Med. 89, 47–53 (2015).
Peskin, A. V. et al. The high reactivity of peroxiredoxin 2 with H2O2 is not reflected in its reaction with other oxidants and thiol reagents. J. Biol. Chem. 282, 11885–11892 (2007).
Low, F. M., Hampton, M. B., Peskin, A. V. & Winterbourn, C. C. Peroxiredoxin 2 functions as a noncatalytic scavenger of low-level hydrogen peroxide in the erythrocyte. Blood 109, 2611–2617 (2007).
Rhee, S. G., Chae, H. Z. & Kim, K. Peroxiredoxins: A historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free Radic. Biol. Med. 38, 1543–1552 (2005).
Wood, Z. A., Schröder, E., Robin Harris, J. & Poole, L. B. Structure, mechanism and regulation of peroxiredoxins. Trends Biochem. Sci. 28, 32–40 (2003).
Cox, A. G., Winterbourn, C. C. & Hampton, M. B. in Methods in Enzymology 474, 51–66 (2010).
Morgan, B. et al. Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes. Nat. Chem. Biol. 12, 437–443 (2016).
Muthuramalingam, M. et al. Multiple redox and non-redox interactions define 2-cys peroxiredoxin as a regulatory hub in the chloroplast. Mol. Plant 2, 1273–1288 (2009).
Seidel, T., Seefeldt, B., Sauer, M. & Dietz, K. J. In vivo analysis of the 2-Cys peroxiredoxin oligomeric state by two-step FRET. J. Biotechnol. 149, 272–279 (2010).
Wolf, H., Barisas, B. G., Dietz, K. J. & Seidel, T. Kaede for detection of protein oligomerization. Mol. Plant 6, 1453–1462 (2013).
Lam, A. J. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat. Methods 9, 1005–1012 (2012).
Edgar, R. et al. Peroxiredoxins are conserved markers of circadian rhythms. Nature 485, 459–464 (2012).
Park, J.-G. et al. Peroxiredoxin 2 deficiency exacerbates atherosclerosis in apolipoprotein E-deficient mice. Circ. Res. 109, 739–749 (2011).
Hu, X. et al. Peroxiredoxin-2 protecs against 6-hydroxydopamine-induced dopaminergic neurodegeneration via attenuation of the ASK1 signaling cascade. J. Neurosci. 31, 247–261 (2011).
Perkins, A. et al. The sensitive balance between the fully folded and locally unfolded conformations of a model peroxiredoxin. Biochemistry 52, 8708–8721 (2013).
Lim, J. B., Barker, K. A., Huang, B. K. & Sikes, H. D. In-depth characterization of the fluorescent signal of HyPer, a probe for hydrogen peroxide, in bacteria exposed to external oxidative stress. J. Microbiol. Methods 106, 33–39 (2014).
Wagner, B. A., Witmer, J. R., van’t Erve, T. J. & Buettner, G. R. An assay for the rate of removal of extracellular hydrogen peroxide by cells. Redox Biol. 1, 210–217 (2013).
Gülden, M., Jess, A., Kammann, J., Maser, E. & Seibert, H. Cytotoxic potency of H2O2 in cell cultures: Impact of cell concentration and exposure time. Free Radic. Biol. Med. 49, 1298–1305 (2010).
Doskey, C. M., Van’t Erve, T. J., Wagner, B. A. & Buettner, G. R. Moles of a substance per cell is a highly informative dosing metric in cell culture. PLoS ONE 10, (2015). https://doi.org/10.1371/journal.pone.0132572
Halvey, P. J. et al. Selective oxidative stress in cell nuclei by nuclear-targeted D-amino acid oxidase. Antioxid. Redox Signal. 9, 807–816 (2007).
Haskew-Layton, R. E. et al. Controlled enzymatic production of astrocytic hydrogen peroxide protects neurons from oxidative stress via an Nrf2-independent pathway. Proc. Natl Acad. Sci. USA 107, 17385–17390 (2010).
Matlashov, M. E., Belousov, V. V. & Grigori, E. How much H2O2 is produced by recombinant D-amino acid oxidase in mammalian cells? Antioxid. Redox Signal. 20, 1039–1044 (2014).
Ezeriņa, D., Morgan, B. & Dick, T. P. Imaging dynamic redox processes with genetically encoded probes. J. Mol. Cell Cardiol. 73, 43–49 (2014).
Bilan, D. S. & Belousov, V. V. HyPer family probes: state of the art. Antioxid. Redox Signal. 24, 731–751 (2015).
Singh, A. et al. Dysfunctional KEAP1-NRF2 interaction in non-small-cell lung cancer. PLoS Med. 3, 1865–1876 (2006).
Solis, L. M. et al. Nrf2 and Keap1 abnormalities in non-small cell lung carcinoma and association with clinicopathologic features. Clin. Cancer Res. 16, 3743–3753 (2010).
Lin, V. S., Dickinson, B. C. & Chang, C. J. Boronate-based fluorescent probes: imaging hydrogen peroxide in living systems. Methods Enzymol. 526, 19–43 (2013).
Malinouski, M., Zhou, Y., Belousov, V. V., Hatfield, D. L. & Gladyshev, V. N. Hydrogen peroxide probes directed to different cellular compartments. PLoS ONE 6, (2011). https://doi.org/10.1371/journal.pone.0014564
Kalyanaraman, B. et al. Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic. Biol. Med. 52, 1–6 (2012).
Winterbourn, C. C. The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells. Biochim. Biophys. Acta 1840, 730–738 (2014).
Stockwell, B. R. et al. Ferroptosis: a regulated cell death nexus linking metabolism, redox biology, and disease. Cell 171, 273–285 (2017).
Xu, W. et al. Role of peroxiredoxin 2 in the protection against ferrous sulfate-induced oxidative and inflammatory injury in PC12 cells. Cell Mol. Neurobiol. 38, 735–745 (2018).
Huang, B. K. & Sikes, H. D. Quantifying intracellular hydrogen peroxide perturbations in terms of concentration. Redox Biol. 2C, 955–962 (2014).
Huang, B. K., Ali, S., Stein, K. T. & Sikes, H. D. Interpreting heterogeneity in the response of cells expressing a fluorescent biosensor to stimulation with hydrogen peroxide. Biophys. J. 109, 2148–2158 (2015).
Laer, K. Van & Dick, T. P. Utilizing natural and engineered peroxiredoxins as intracellular peroxide reporters. Mol. Cells 39, 46–52 (2016).
Moon, J. C. et al. Oxidative stress-dependent structural and functional switching of a human 2-Cys peroxiredoxin isotype II that enhances HeLa cell resistance to H2O2-induced cell death. J. Biol. Chem. 280, 28775–28784 (2005).
Rinalducci, S., D’Amici, G. M., Blasi, B. & Zolla, L. Oxidative stress-dependent oligomeric status of erythrocyte peroxiredoxin II (PrxII) during storage under standard blood banking conditions. Biochimie 93, 845–853 (2011).
Stöcker, S., Laer, K. Van, Mijuskovic, A. & Dick, T. P. The conundrum of hydrogen peroxide signaling and the emerging role of peroxiredoxins as redox relay hubs. Antioxid. Redox Signal. 28, 558–573 (2018).
Sobotta, M. C. et al. Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling. Nat. Chem. Biol. 11, 64–70 (2014).
Adams, D. J. et al. Discovery of small-molecule enhancers of reactive oxygen species that are nontoxic or cause genotype-selective cell death. ACS Chem. Biol. 8, 923–929 (2013).
T.F.L acknowledges support from the Haas Family Fellowship in Chemical Engineering. B.K.H. and J.B.L. acknowledge support from the NSF GRFP. S.J.M. acknowledges support from a Samsung Fellowship. H.D.S. acknowledges support from a Burroughs Wellcome Fund Career Award at the Scientific Interface and the Joseph R. Mares endowed professorship. We thank Kassi Stein for valuable input in both experimental design and manuscript preparation.
The authors declare no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Langford, T.F., Huang, B.K., Lim, J.B. et al. Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat Commun 9, 3145 (2018). https://doi.org/10.1038/s41467-018-05557-y
Green and controllable synthesis of multi-heteroatoms co-doped graphene fiber as flexible and biocompatible microelectrode for in situ electrochemical detection of biological samples
Sensors and Actuators B: Chemical (2021)
Redox Biology (2021)
Redox-sensitive mapping of a mouse tumor model using sparse projection sampling of electron paramagnetic resonance
Antioxidants & Redox Signaling (2021)
Direct sensing of peroxynitrite anion at sensitive hollow tubular organic conjugated microporous polymers modified electrode. Application to selective analysis of ROS and RNS in cells
Sensors and Actuators B: Chemical (2020)
Proceedings of the National Academy of Sciences (2020)