Optic atrophy 1 (OPA1) is a mitochondrial inner membrane protein that has an important role in mitochondrial fusion and structural integrity. Dysfunctional OPA1 mutations cause atrophy of the optic nerve leading to blindness. Here, we show that OPA1 has an important role in the innate immune system. Using conditional knockout mice lacking Opa1 in neutrophils (Opa1N∆), we report that lack of OPA1 reduces the activity of mitochondrial electron transport complex I in neutrophils. This then causes a decline in adenosine-triphosphate (ATP) production through glycolysis due to lowered NAD+ availability. Additionally, we show that OPA1-dependent ATP production in these cells is required for microtubule network assembly and for the formation of neutrophil extracellular traps. Finally, we show that Opa1N∆ mice exhibit a reduced antibacterial defense capability against Pseudomonas aeruginosa.
Besides intracellular killing, neutrophils are able to exert an antibacterial activity in the extracellular space by the formation of so-called neutrophil extracellular traps (NETs)1,2. NETs consist of DNA and granule proteins and are able to bind and to kill extracellular pathogens. Thus, NETs represent an important element of the innate immune response and they are seen in association with many infectious, allergic, and autoimmune diseases3,4,5. It should be noted that such extracellular DNA traps able to kill bacteria can also be formed by eosinophils6 and basophils7. Although the functional importance of NETs is generally accepted, the origin of the DNA scaffold in NETs, as well as the mechanism of their generation, remains unclear and a matter of dispute.
Since we had observed that neutrophils and other granulocytes release mitochondrial DNA (mtDNA) in the formation of extracellular DNA traps3, we hypothesized that mitochondrial dynamics might be important for mtDNA release. Five members of the GTPase dynamin family, namely mitofusin-1 (MFN1), mitofusin-2 (MFN2), optic atrophy 1 (OPA1), mitochondrial fission 1 (FIS1), and dynamin-related protein 1 (DRP1) are known as the “mitochondria-shaping” proteins which regulate the fusion and fission of mitochondria8. OPA1 is anchored to the mitochondrial inner membrane, and, together with MFN1 and MFN2, which are located in the outer mitochondrial membrane, it controls the mitochondrial fusion process9. Interestingly, and independent of its role in mitochondrial fusion, OPA1 is also responsible for maintaining the mitochondrial cristae junctions tight, thus allowing efficient oxidative respiration10,11,12. Genetic depletion of OPA1 resulted in reduced cell growth and lowered mitochondrial membrane potential, as well as in defective cellular respiration and metabolism13,14. Patients with mutated OPA1 can develop autosomal dominant optic atrophy (ADOA), which leads to loss of bilateral visual function within the first two decades of life, or may present with additional signs of peripheral neuropathy, deafness, cerebellar ataxia, paraparesis, and myopathy15,16. Reduction of OPA1 activity has also been associated with other neurodegenerative diseases, such as Alzheimer, Huntington, and Parkinson diseases17.
In this paper, we demonstrate that the lack of OPA1, or mutations in the OPA1 gene, can also result in functional defects outside of the nervous system. Specifically, we show that OPA1 is required for ATP production through glycolysis in neutrophils, cells which are known to contain a modest number of mitochondria, but are unable to perform mitochondrial respiration18. ATP production in neutrophils is required for the assembly of the microtubule network. Furthermore, this network has recently been demonstrated to be essential for NET production19. Neutrophils lacking other members of GTPase dynamin family exhibit no such defect in NET formation, suggesting that the OPA1 effects on neutrophils are a consequence of its role in maintaining the mitochondrial cristae junctions tight and thus likely to be independent of mitochondrial fusion. Moreover, mice with Opa1 knockout neutrophils demonstrate defects in antibacterial defense owing to their inability to form NETs under in vivo conditions. Taken together, the findings presented here provide a significant step forward in understanding OPA1 mitochondrial functions in non-neuronal cells and extend the impact of these functions to the innate immune system.
OPA1-deficient neutrophils are unable to form NETs
To test whether members of GTPase dynamin family are involved in the process of extracellular DNA release from neutrophils, we used a small hairpin RNA (shRNA)-based approach. To this end, lentivirus particles containing five different human shRNA sequences for each candidate gene, namely; DRP1, FIS1, MFN1, MFN2, and OPA1 (Mission shRNA, Sigma-Aldrich) were transduced into human myeloid leukemia cells (PLB-985). Similarly, mouse shRNAs were used in Hoxb8-immortalized mouse progenitor cells (Hoxb8 neutrophils)20. After antibiotic selection and cloning of single cells, transduced clones were tested for the down-regulation of target genes using qPCR (Supplementary Fig. 1a, b, left panels). Cells with the lowest expression of target genes were differentiated to mature neutrophils and examined for their ability to release DNA following GM-CSF priming and subsequent C5a stimulation21. Reduction of OPA1 mRNA expression, but not any of the other dynamin-like GTPases, was associated with a complete inability to release DNA with either differentiated human myeloid leukemia cells or mouse Hoxb8 neutrophils (Supplementary Fig. 1a, b, right panels).
To verify the relevance of OPA1 for DNA release and NET formation in primary human neutrophils, we isolated blood neutrophils from two patients of the same family (father and son), both suffering from autosomal dominant optic atrophy disease (ADOA). They harbor the heterozygous synonymous mutation c.1140G>A/ p.(Glu380Glu) resulting in exon 11 skipping in the original isoform identified (NM_015560.2) (Fig. 1a)22. While the neutrophils of the father released extracellular DNA following combined GM-CSF/C5a stimulation in the same way as control neutrophils, activation of neutrophils from the son failed to cause DNA release (Fig. 1b), in spite of their similar level of OPA1 protein expression (Fig. 1c). Although it remains unclear why the neutrophils of father and son behaved differently, we concluded that mutated OPA1 can result in a functional defect with respect to extracellular DNA release in primary human neutrophils.
It has been speculated that an OPA1 deficiency might cause mtDNA instability in neurons, leading to reduced mtDNA levels23,24. To test this hypothesis, we compared the mtDNA content by analyzing the levels of the mitochondrial gene ATP synthase protein 8 (MT-ATP8) and the mitochondrial D-loop region compared to the single copy β2 microglobulin (B2M) nuclear gene25 and observed no differences between neutrophils of ADOA patients and healthy controls (Fig. 1d). These findings are in agreement with a previous report demonstrating that white blood cells of ADOA patients exhibit a normal content of mtDNA26. Therefore, it seems unlikely that the failure to release DNA in OPA1 defective neutrophils is the consequence of reduced mtDNA levels.
Since ADOA exhibits heterogeneous phenotypes12,27 and as it is a rare disease, making it difficult to obtain primary neutrophils from patients, we pursued a genetic approach to investigate more closely the role of OPA1 in DNA release and NET formation. Mouse and fly models that carry homozygous Opa1 mutations are embryonically lethal28. Therefore, we generated Opa1flox/flox-Lyz2Cre/Cre mice (designated Opa1N∆ mice hereafter) to study the effects of Opa1 deficiency in neutrophils (Supplementary Fig. 2a). Mature bone marrow neutrophils of Opa1N∆ mice displayed a complete OPA1 knockout at the protein level (Supplementary Fig. 2b). In contrast to neutrophils from control Lyz2Cre/Cre mice, Opa1N∆ primary mouse neutrophils did not exhibit any detectable DNA release upon GM-CSF priming and activation with C5a, with LPS treatment, or following co-culture with E. coli–GFP (Fig. 1e, left panel). To quantify the dsDNA released by activated neutrophils, we collected the culture supernatants and measured the amount released in the supernatant using PicoGreen fluorescent dye. In contrast to Lyz2Cre/Cre mice, neutrophils derived from Opa1N∆ mice were unable to release dsDNA upon activation (Fig. 1e, right panel). We also tested other activating reagents previously reported to induce NET formation such as PMA, P. aeruginosa in co-culture, or nucleic acid–containing immune complexes (ICs) produced by combining the small nuclear ribonucleoprotein (smRNP) antigen with either SLE sera (RNP ICs-SLE) or with anti-damaged-DNA/RNA antibody (RNP-ICs-Ab)29. None of these stimuli could trigger NET formation in Opa1N∆ neutrophils (Supplementary Fig. 2c). To exclude any effect of Cre recombinase on extracellular DNA release, we also analyzed Opa1flox/flox neutrophils and observed no difference as compared to Lyz2Cre/Cre neutrophils (Supplementary Fig. 2d).
We next investigated functional consequences of the lack of DNA release in Opa1N∆ neutrophils in terms of NET formation. Since granule proteins are essential components of NETs2,21,30, we performed immunofluorescence microscopy studies in which we searched for the presence of elastase within the released DNA scaffold of activated mouse neutrophils. In activated control Lyz2Cre/Cre neutrophils, we found extracellular DNA fibers co-localizing with elastase (Supplementary Fig. 2e). In contrast, with Opa1N∆ neutrophils, we were unable to detect elastase-containing extracellular structures.
In summary, we report that neutrophilic cell lines and primary human or mouse neutrophils deficient in functional OPA1 are unable to form the extracellular DNA scaffolds as seen in NETs.
OPA1-deficient neutrophils exhibit disruption of the microtubule network
Mitochondrial localization analysis revealed a wide distribution in the cytoplasm of resting Opa1N∆ neutrophils, whereas in control Lyz2Cre/Cre neutrophils, mitochondria were concentrated in the perinuclear region (Supplementary Fig. 3a). Following combined GM-CSF/C5a stimulation of control neutrophils, the vast majority of mitochondria translocated toward the plasma membrane. In contrast, in stimulated Opa1N∆ neutrophils, mitochondria remained widely distributed showing no change in location (Supplementary Fig. 3a). This finding points to a possible defect in microtubule network dynamics31. Further suggestive of a microtubule defect is the observation that stimulated Opa1N∆ neutrophils also exhibit a failure in degranulation (Supplementary Fig. 3b, c), a process which is known to be dependent on microtubule assembly32.
Therefore, we investigated the microtubule network in control Lyz2Cre/Cre and Opa1N∆ neutrophils following activation and observed a disrupted microtubule network associated with OPA1 deficiency (Fig. 2a). Quantification of the microtubule network assembly demonstrated significant differences between Opa1N∆ neutrophils compared to Opa1flox/flox and Lyz2Cre/Cre control neutrophils following GM-CSF/C5a stimulation (Supplementary Fig. 4a, b). Reduced microtubule network formation in Opa1N∆ neutrophils was also observed following activation with GM-CSF/RNP ICs-Ab, GM-CSF/RNP ICs-SLE, PMA, or P. aeruginosa as compared to activated Lyz2Cre/Cre control neutrophils (Supplementary Fig. 4c). Inhibition of the microtubule network formation was also observed in human neutrophils expressing functionally inactive OPA1 (Fig. 2b). A quantitative analysis demonstrates that OPA1-defective human neutrophils are unable to assemble the microtubule network following combined GM-CSF/C5a stimulation (Supplementary Fig. 5a). To investigate the role of the microtubule network in DNA release, we used nocodazole and taxol, both known as highly efficient microtubule-disrupting agents33. As previously reported19, both drugs completely prevented the formation of the microtubule network (Fig. 2c) as well as interdicting DNA release in stimulated normal human neutrophils (Fig. 2d). Taken together, these results indicate that OPA1 regulates microtubule network assembly which is required for the translocation of mitochondria, for degranulation, and for DNA release in activated mouse and human neutrophils.
On the other hand, Opa1N∆ neutrophils exhibited normal production of reactive oxygen species (ROS) upon stimulation, excluding the possibility that OPA1 regulates the NADPH oxidase which is required for NET formation (Supplementary Fig. 4d)2,34. In addition, Opa1N∆ neutrophils showed no abnormality with regard to actin polymerization upon activation (Supplementary Fig. 4e). Normal actin polymerization was also observed in human neutrophils expressing functionally inactive OPA1 (Supplementary Fig. 5b), confirming the findings obtained with primary mouse neutrophils. Summarizing, OPA1-deficient neutrophils are unable to form a microtubule network, but exhibit normal ROS production and actin polymerization under the same activation conditions.
OPA1-deficient neutrophils exhibit decreased ATP production
Super-resolution microscopy allowed us to clearly visualize a mitochondrial fragmentation in Opa1N∆ neutrophils that was not seen in DNA-releasing control Lyz2Cre/Cre neutrophils in which mitochondria remained interconnected (Fig. 2a). Moreover, in contrast to normal human neutrophils that exhibit long filamentous mitochondria in the absence of stimulus, we also observed mitochondrial fragmentation in neutrophils of ADOA patients in both activated and resting conditions (Fig. 2b). These findings confirm previously published work suggesting a role for OPA1 in mitochondrial fusion11.
It has been reported that the tightness of cristae junctions correlates with oligomerization of OPA110,11. On the other hand, genetic depletion of OPA1 leads to disorganization of the cristae, resulting in problems with its assembly and with the stability of respiratory chain complexes and mitochondrial respiratory function10,35. To image mitochondria in Opa1N∆ neutrophils, we used conventional transmission electron microscopy (TEM). While the number of mitochondria appeared to be independent of OPA1 expression (Fig. 3a, b), a finding which was supported by our mtDNA measurements (Fig. 3c), we also investigated mitochondrial transcription factor A (TFAM) expression as a measure of mtDNA levels. Equal levels of TFAM expression in control and Opa1N∆ neutrophils were observed (Fig. 3d), also confirming indirectly that the mtDNA levels are unchanged in the absence of OPA1. Morphometric analysis revealed a diminished average mitochondrial length (Fig. 3a, e) and an increased width of the cristae (Fig. 3a, f) in Opa1N∆ compared to Lyz2Cre/Cre neutrophils. Upon combined GM-CSF/C5a stimulation, the number of mitochondria increased owing to fission to a smaller average size in control neutrophils, while in Opa1N∆ neutrophils, the number and size of mitochondria did not change significantly (Fig. 3a and Supplementary Fig. 6).
The increased width of the cristae as a consequence of OPA1 ablation is believed to be associated with an impaired respiration complex35. In addition, previously published work reported that muscle cells from adult ADOA patients displayed reduced ATP synthesis, suggesting dysregulation of mitochondrial metabolism as a consequence of OPA1 dysfunction36. Therefore, we analyzed the total cellular ATP level in Opa1N∆ neutrophils and observed a reduction as compared to Lyz2Cre/Cre neutrophils (Fig. 3g).
Complex I activity regulates neutrophil glycolysis and functions
To test whether diminished ATP levels could contribute to the disruption of the microtubule network and the failure of DNA release, exogenous ATP or nicotinamide mononucleotide (NMN; to rescue NAD+ levels, see below) was added to the cells 30 min before physiological activation of the neutrophils with GM-CSF/C5a. Indeed, addition of exogenous ATP or NMN were able to restore the ability of Opa1N∆ neutrophils to form a normal microtubule network (Fig. 4a) and to release extracellular DNA following activation (Fig. 4b). Quantification of dsDNA in neutrophil supernatants confirmed the DNA release by Opa1N∆ neutrophils in the presence of exogenous ATP or NMN (Fig. 4b, right panel). Similar results for DNA release were obtained following stimulation of neutrophils with PMA, P. aeruginosa or immune complexes (Supplementary Fig. 7a, b).
It should be noted that addition of exogenous ATP alone did not trigger DNA release either in human (Supplementary Fig. 8a) or in mouse neutrophils (Supplementary Fig. 8b), largely excluding the possibility that Opa1N∆ neutrophils release DNA as a consequence of P2X receptor activation. Moreover, the addition of exogenous ATP had no effect on the viability of either human (Supplementary Fig. 8c) or mouse neutrophils (Supplementary Fig. 8d). We also obtained no evidence for increased apoptosis of Opa1N∆ compared to Lyz2Cre/Cre neutrophils (Supplementary Fig. 8d); a pan-caspase inhibitor failed to restore DNA release in GM-CSF/C5a-activated Opa1N∆ neutrophils (Supplementary Fig. 8e), suggesting that, as an explanation for the inability of Opa1N∆ neutrophils to form NETs, a difference in apoptosis regulation can be excluded.
In order to determine the source(s) for ATP production in human and mouse neutrophils, we applied specific inhibitors blocking different steps of mitochondrial respiration and glycolysis and measured total cellular ATP concentrations. After 70 min treatment of human neutrophils with the mitochondrial ATP synthase inhibitor oligomycin A or with the glycolysis inhibitor 2-deoxy-D-glucose (2-DG), we observed that inhibition of glycolysis clearly reduced ATP production, whereas inhibition of mitochondrial ATP synthase had no effect on neutrophil ATP levels (Fig. 5a). Moreover, we analyzed the ATP/ADP ratio as a measure for the energetic state of the cell and observed that the ratio was reduced by 2-DG in control Lyz2Cre/Cre mouse neutrophils, but the reduced levels already attained in Opa1N∆ neutrophils were not further reduced (Fig. 5b). Following GM-CSF/C5a stimulation, control neutrophils exhibit a reduced ATP/ADP ratio as a consequence of reduced ATP production, perhaps owing to the energy requirement for microtubule network rearrangements as previously suggested37. The fact that the fall in ATP concentration following neutrophil activation is not balanced by any measurable increase in ADP or AMP concentration has been reported previously38. Our data support the concept that glycolysis is the main source of ATP production in neutrophils18 and indicate that Opa1N∆ neutrophils exhibit a defect in glycolysis.
Despite the fact that glycolysis in the cytoplasm is the main source of ATP production in neutrophils, surprisingly, the mitochondrial complex I inhibitor rotenone also reduced ATP levels (Fig. 5a), indicating a link between glycolysis and the mitochondrial complex I pathway for ATP production in neutrophils. To examine whether Opa1 depletion has any effect on complex I activity in neutrophils, we analyzed the enzymatic activities of the mitochondrial complex I (CI) and complex III (CIII) normalized to citrate synthase (CS). The results clearly show that both resting and activated Opa1N∆ neutrophils exhibit a significant defect in CI, but not CIII, activity (Fig. 5c), and in a diminished NAD+/NADH ratio (Fig. 5d). In the process of glycolysis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) requires two NAD+ molecules per glucose molecule. Therefore, NAD+ availability is an important determinant for the rate of glycolysis39,40. Consistent with reduced glycolysis, we have also found reduced lactate levels in Opa1N∆ as compared to Lyz2Cre/Cre neutrophils (Fig. 5e).
To better understand the basal level of glycolytic function in Opa1N∆ and control Lyz2Cre/Cre mouse neutrophils, the extracellular acidification rate (ECAR; an indicator of aerobic glycolysis) and oxygen consumption rate (OCR; an indicator of OXPHOS) were analyzed. Consistent with the lactate findings (Fig. 5e), we found a general decrease in basal ECAR in Opa1N∆ compared to control Lyz2Cre/Cre mouse neutrophils (Supplementary Fig. 9a, left panel). A lower ECAR in Opa1N∆ neutrophils was also seen after blocking CI or CV activities or adding the mitochondrial uncoupling agent p-triflouromethoxyphenylhydrazone (FCCP) (Supplementary Fig. 9a, right panel). Inhibition of CI activity had no effect in Opa1N∆ neutrophils (Supplementary Fig. 9a, left panel), in agreement with the observation that these cells already exhibit a defect in CI activity (Fig. 5c). The addition of 2-DG abolished extracellular acidification in both Opa1N∆ and control Lyz2Cre/Cre neutrophils (Supplementary Fig. 9a, left and right panels), confirming that neutrophils entirely depend on glycolysis. In agreement with this concept, both Opa1N∆ and control Lyz2Cre/Cre neutrophils exhibit very low OCR (Supplementary Fig. 9b, left panel). The limited OCR in neutrophils is even further reduced in Opa1N∆ neutrophils which, however, is an observation that gains statistical significance only at maximal respiration after adding FCCP (Supplementary Fig. 9b, right panel). The discrepancies between the clearly reduced CI activity and the only mildly affected OCR might be explained by the fact that mitochondria of neutrophils can receive electrons from glycolysis via glycerol-3-phosphate through the glycerophosphate dehydrogenase complex in the Q cycle41. Taken together, our data suggest that a lack of OPA1 impairs complex I activity, reducing available NAD+ levels, and hence the rate of glycolysis, resulting in reduced ATP production.
Since we observed a defect in the formation of microtubules and NETs following activation of OPA1-deficient human and mouse neutrophils, we investigated whether a pharmacological block in glycolysis and mitochondrial CI activity would also interdict the microtubule network assembly, thus resulting in inhibition of DNA release. Indeed, we found that blocking glycolysis (2-DG) or CI (rotenone or piericidin A), but not CIII (antimycin A) or ATP synthase (oligomycin A), resulted in an inability of normal mouse (Fig. 6a) and human neutrophils (Supplementary Fig. 10) to form microtubule networks or to release DNA upon combined activation with GM-CSF and C5a (Fig. 6b). To prove that ATP is sufficient to rescue the formation of the microtubule network and DNA release, we added either ATP or NMN to GM-CSF/C5a-activated control mouse neutrophils pre-treated with rotenone, piericidin A or 2-DG. Indeed, both exogenous ATP and NMN resulted in successful activation of neutrophils in spite of pharmacological block on CI activity or overall glycolysis (Fig. 6a, b; lower panels). These data support the concept that microtubule network assembly and DNA release depend on ATP generated by glycolysis, for which an OPA1-dependent mitochondrial complex I activity is required.
To demonstrate the importance of OPA1 for functional NET formation, we performed bacterial killing assays in vitro. We observed reduced bacterial killing of P. aeruginosa and E. coli–GFP by Opa1N∆ as compared to Lyz2Cre/Cre neutrophils (Fig. 7a). Moreover, addition of DNase I lowered bacterial killing by Lyz2Cre/Cre neutrophils to levels similar to Opa1N∆ neutrophils. Conversely, DNase I had no effect on Opa1N∆ neutrophils, indicating that the lack of DNA release constitutes the principal cause of the reduced antibacterial activity of Opa1N∆ neutrophils (Fig. 7a). To investigate the importance of mitochondrial complex I and complex III activities, we pre-treated Lyz2Cre/Cre neutrophils with rotenone and antimycin A. Rotenone, but not antimycin A, reduced the neutrophil killing activity, demonstrating the importance of complex I activity for functional NET formation. In contrast, rotenone had no effect on Opa1N∆ neutrophils (Fig. 7a). Importantly, exogenous ATP or NMN rescued the defective bacterial killing activity of Opa1N∆ neutrophils (Fig. 7a). To exclude the possibility that a failure of P. aeruginosa and E. coli–GFP killing by Opa1N∆ neutrophils might be due to a lack of phagocytic activity, we performed phagocytosis assays using opsonized E. coli-GFP. Equal phagocytic activities were observed in Opa1N∆ and Lyz2Cre/Cre neutrophils (Fig. 7b), suggesting that phagocytosis is largely independent of OPA1.
Opa1 N∆ mice exhibit a reduced antibacterial defense capability
NETs can bind and kill bacteria and fungi in the extracellular space1,21. To define the physiological importance of OPA1 in shaping the outcome of a bacterial lung infection, mice were intranasally inoculated with P. aeruginosa42. After 18 h, the median bacterial counts in the lungs of Opa1N∆ mice were higher than for Lyz2Cre/Cre control mice (Fig. 7c). Moreover, systemic infection was more frequently observed in Opa1N∆ mice, as shown by significantly higher median CFU counts in the spleen (Fig. 7d).
Neutrophil recruitment is a pivotal event of the response to P. aeruginosa infection42,43. To quantify neutrophil infiltration, lung homogenates were assessed for the neutrophil-specific enzyme myeloperoxidase activity (MPO). After 18 h infection, MPO enzymatic activity levels in lung homogenates of Opa1N∆ mice were higher than in Lyz2Cre/Cre control mice, suggesting that there is no defect in the neutrophil recruitment to the lungs of Opa1N∆ mice (Fig. 7e).
To analyze the formation of NETs, lung tissues were investigated following staining of DNA and MPO. As expected, following infection, lung sections revealed the presence of NETs in Lyz2Cre/Cre control mice. In contrast, NET formation in Opa1N∆ mice was largely abolished (Fig. 7f). Counting the numbers of infiltrating neutrophils revealed again evidence for increased neutrophil infiltration in the lungs in Opa1N∆ compared to control mice (3917 ± 963 versus 1742 ± 319 neutrophils per mm2; p < 0.05). We additionally performed an automated analysis to quantify neutrophil numbers, confirming increased neutrophil infiltration in Opa1N∆ compared to Lyz2Cre/Cre control mice (Fig. 7f, second right panel). Taken together, despite increased neutrophil recruitment to the site of infection and normal phagocytic activity, Opa1N∆ mice fail to resolve bacterial lung infections, consistent with the lack of NET formation.
Neutrophils are rapidly recruited in tissues during infections to eliminate pathogens44, not only by phagocytosis, but also in the extracellular space by the formation of NETs1. Currently, there is a dispute in the literature regarding the source of extracellular DNA employed for the formation of NETs and whether there is a requirement for neutrophil death in NET formation45. Consequently, the molecular mechanism of NET formation remains unclear. Another matter of contradiction is the question of the functional significance of mitochondria in neutrophils. Since mitochondrial respiration is low in these cells, it has been suggested that neutrophils require mitochondria just for undergoing apoptosis18. On the other hand, it has been demonstrated that neutrophils possess a highly developed mitochondrial network that is important during infection and inflammation46,47. Here, we complement this body of evidence, extending a role of mitochondria in NET formation. Specifically, we report that mitochondrial complex I activity indirectly regulates glycolysis, which is required for microtubule network assembly and functional NET formation both in vitro and in vivo.
Recently, several researchers report release of mtDNA during NET formation, supporting our earlier findings2. For instance, mtDNA release by neutrophils has been intensely studied in systemic lupus erythematous (SLE) patients48. In this autoimmune disease, mtDNA acts as the most potent activator of plasmacytoid dendritic cells (pDCs) and the type I interferon (IFN) pathway49. Moreover, following activation of neutrophils with ribonucleoprotein immune complexes (RNP ICs), mitochondria were shown to be mobilized toward the cell surface where they released oxidized mtDNA in a mitochondrial ROS-dependent manner29. Additionally, a direct fusion of mitochondrial membranes with the plasmalemma as a potential mtDNA release mechanism in neutrophils has been postulated50. However, no conclusive molecular mechanism of mtDNA release has yet been reported.
To investigate the role of mitochondrial dynamics in NET formation, we have used super-resolution, confocal and TEM microscopy to analyze the interconnected tubular mitochondrial network in healthy human and mouse neutrophils. Down-regulation of different genes known to be responsible for mitochondrial fusion and fission using shRNA has pointed to an important role for OPA1 in facilitating DNA release. OPA1 is important for mitochondrial biogenesis and fusion35. Moreover, OPA1 regulates the shape of the mitochondrial cristae and their “tightness”, a function needed for maintaining ion gradients, hence for overall respiratory efficiency35. Human and mouse neutrophils devoid of functional OPA1 protein display not only a fragmented mitochondrial morphology, but also lack microtubule networks which are required for mitochondrial translocation, the process of degranulation, and the ability to form functional NETs upon activation. It should be noted that OPA1 cleavage in cerebellar granule neurons (CGNs) coincides with extensive mitochondrial fragmentation and disruption of the microtubule network31. While we could pharmacologically demonstrate a requirement for the microtubule network for NET formation, we do not formally demonstrate why this is the case. However, disorientation of mitochondria in cells exhibiting a disrupted microtubule array could explain the lack of extracellular DNA release by ADOA and Opa1N∆ neutrophils.
Microtubule polymerization is an energy-dependent process37. It has been demonstrated that ATP is able to polymerize microtubules through an interaction at the exchangeable GTP site51 and that reducing ATP synthesis leads to decreased microtubule stability52. We demonstrate that low levels of cellular ATP in OPA1-deficient neutrophils are insufficient for microtubule network and NET formation, a situation that could be reversed by addition of exogenous ATP or NMN which rescued NAD+ levels. Surprisingly, however, actin polymerization and phagocytosis did not appear to be influenced by the lack of OPA1, suggesting that these events require less ATP. But why should OPA1, a mitochondrial protein known to be involved in mitochondrial ATP production, be important in neutrophils, which depend on glycolysis for ATP? Using neutrophils deficient in Opa1, we observed that a lack of OPA1 caused remodeling of mitochondrial cristae structure, resulting in reduced mitochondrial complex I activity and lower levels of NAD+, which is a key participant in glycolysis40. Therefore, diminution of glycolysis as the main ATP production pathway in neutrophils is the reason for low cellular ATP levels in ADOA and Opa1N∆ neutrophils, explaining their inability to form microtubule networks and functional NETs. These findings were confirmed with a pharmacological approach using inhibitors of mitochondrial complex I activity and glycolysis.
We also provide data showing that OPA1 function is important for anti-microbial defense by neutrophils in an in vivo model of P. aeruginosa lung infection. Mice lacking Opa1 in their neutrophils and macrophages did not form NETs and were less able to eliminate bacteria from the lungs. Moreover, these mice exhibited a greater probability of systemic spread of infection. Therefore, pharmacological activation of OPA1 in neutrophils might be a new option for fighting bacterial and fungal infections. Moreover, clinicians should be aware of the possibility that ADOA patients may exhibit an increased risk of infections. Taken together, the findings in this study provide further evidence for a role of mitochondria in the biology of neutrophils, particularly for NET formation, and extend the impact of an OPA1 function to the innate immune response.
Granulocyte/macrophage colony-stimulating factor (GM-CSF) was provided by Novartis Pharma (Nürnberg, Germany). Human and mouse C5a were purchased from Hycult Biotech (Uden, the Netherlands). MitoSOX Red, Alexa Fluor 488 Phalloidin, MitoTracker Orange CM-H2TMRos, SYTO13, propidium iodide, CellMask Deep Red, Prolong Gold mounting media, Hoechst 33342, the Quant-iTTMPicoGreen®dsDNA Assay Kit and RPMI 1640/GlutaMAX medium were obtained from ThermoFisher Scientific (distributed by LuBioScience GmbH, Lucerne, Switzerland). X-VIVO 15 medium was from Lonza (Walkersville, MD, USA). Polyvalent human IgG was a kind gift from CSL Behring (Bern, Switzerland). Normal goat (Cat # NBP2-23475, dilution 1:400), rat (Cat # NBP2-33356, dilution 1:400), and rabbit (Cat # NBP1-71681, dilution 1:400) sera were purchased from Novus Biologicals, Abingdon, UK. ChromPure human IgG (Cat # 009-000-003, dilution 1:1000) and normal mouse sera (Cat # 015-000-120, dilution 1:400) were obtained from Milan Analytica AG (Rheinfelden, Switzerland). Bovine serum albumin (BSA), glutaraldehyde, saponin, sodium borohydride (NaBH4), ATP, lipopolysaccharide (LPS, 055:B5), dihydrorhodamine 123 (DHR), 10% formalin solution, β-nicotinamide adenine dinucleotide (NADH), β-nicotinamide mononucleotide (NMN), piericidin A (PA), potassium cyanide (KCN), rotenone (Rot), antimycin A (Anti A), oligomycin A (Oli A), 2-deoxy-d-glucose (2-DG), nocodazole, taxol, propidium iodide, cytochalasin B, tris base, ubiquinone1, bovine serum albumin (fatty acid free), sodium dodecyl sulfate (SDS), protease inhibitor cocktail, tryptic soy broth, and agar, Luria broth base (LB), o-dianisidine dihydrochloride, Triton X-100, N,N-dimethylformamide (DMF), and Nutridoma-SP were obtained from Sigma-Aldrich (Buchs, Switzerland). The NucleoSpin tissue kit for genomic DNA extraction was purchased from Macherey Nagel AG (Oensingen, Switzerland). IQ SYBR Green Supermix was from Bio-Rad Laboratories AG (Cressier, Switzerland). The Mitochondria Isolation Kit and Pierce BCA protein assay kit were from ThermoFisher Scientific as distributed by LuBioScience GmbH (Luzern, Switzerland). The CellTiter-GloR luminescent assay, ADP/ATP-Glo™ bioluminescent assay, and NAD/NADH-Glo™ bioluminescent assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides as well as the Lactate-Glo™ bioluminescent assay were from Promega AG (Dübendorf, Switzerland). Deoxyribonuclease (DNase I) was from Worthington Biochemical Corporation (Lakewood, NJ, USA). Heparinized disposable glass pipettes were supplied by (Provet AG, Lyssach, Switzerland). The Mouse Neutrophil Enrichment Kit was from Stemcell Technologies (Grenoble, France). Pancoll-Human was purchased from PAN-Biotech GmbH (Aidenbach, Germany). 12-mm high-quality glass-cover slips for confocal microscopy analysis (Cat. # 1001/12) were purchased from Karl Hecht “Assistent” GmbH (Sondheim/Rhön, Germany).
Opa1 N∆ mice
We crossed mice bearing an Opa1flox allele, in which, following Cre-mediated recombination, the deletion of Opa1 exons 2 and 3 resulted in an aberrant exon1-exon4 transcript35, with knock-in mice expressing Cre recombinase under the control of the lysozyme 2 endogenous promoter (Lyz2Cre/Cre mice). Lysozyme 2 is a marker of myeloid cells predominantly active in mature neutrophils and macrophages, and appears during late myeloid differentiation. The resulting Opa1flox/flox_Lyz2Cre/Cre mice were designated Opa1N∆ mice. Lyz2Cre/Cre mice or sometimes, additionally, Opa1flox/flox mice were used as controls in all experiments. Mouse genomic DNA was tested by PCR to verify the genotypes and homozygosity of Opa1flox/flox and Lyz2Cre/Cre alleles in Opa1N∆ mice. All mouse experiments were approved by the Cantonal Veterinary Office of Bern, Switzerland (permission # BE5/15).
Genomic PCR for mice deficient in Opa1
For genotyping of mice, genomic DNA was extracted from an ear punch of mice using the NucleoSpin® Tissue Kit. PCR amplification was performed with 25 ng of total DNA using the following mouse primers (Opa1 forward: 5′-CAG TGT TGA TGA CAG CTC AG-3′; and reverse: 5′- CAT CAC ACA CTA GCT TAC ATT TGC-3′) and (Lyz2 forward: 5′-CCC AGA AAT GCC AGA TTA CG-3′, 5′-CTT GGG CTG CCA GAA TTT CTC-3′, and reverse: 5′-TTA CAG TCG GCC AGG CTG AC-3′), with SYBR Green Supermix. The samples were placed on a PCR thermo cycler amplified at 94 °C for 5 min, annealing at the required temperature for each primer for 30 s and 60 °C for 30 s with an extension at 72 °C for 1 min, for 40 cycles. The PCR-amplified products were separated on 2% polyacrylamide gels.
cDNA analysis for exon 11 skipping in ADOA patients’ neutrophils
For OPA1 (NM_015560.2) exon 11 skipping, the following primer sequences were used: 3′- GTG CTG GAA AGA CTA GTG TGT TG -5′ and 3′-AGT ATG ATG GCA TTA GGA TTC TG-5′. Primers were synthesized by Microsynth AG (Balgach, Switzerland). Total RNA was extracted using the QIAGEN RNAeasy Kit. cDNA synthesis was performed using random hexamers with Invitrogen SuperScript II. The samples were placed on a PCR thermo cycler, amplified at 94 °C for 5 min, annealing at required temperature for each primer for 30 s and 60 °C for 30 s with an extension of 72 °C for 1 min, for 40 cycles. The PCR-amplified products were separated on 1.5% agarose gels.
Isolation of mouse bone marrow and human blood neutrophils
All mice were analyzed between 8 and 13 weeks of age. Blood was collected from the retro-orbital sinus of sedated mice, using heparinized disposable glass pipettes. Total leukocyte cell counts in blood were measured on a Scil Vet ABC Hematology Analyzer (Horiba Medical, Montpellier, France). For serum preparation, the blood was left to clot for 2 h at room temperature and centrifuged (20 min, 960 × g, 4 °C). Bone marrow cells were collected by flushing the femur with 5 ml of 2% FCS in PBS, using a 26-gauge needle, and filtering through a sterile 70-μm mesh nylon cell strainer. Bone marrow single-cell suspensions were then washed with medium and the cells counted with a hemocytometer, using the Türk’s staining solution (Dr. Grogg Chemie AG, Stettlen, Switzerland). Primary bone marrow neutrophils were isolated by the negative selection technique, using an EasySep mouse neutrophil enrichment kit. Neutrophil purity was always higher than 90% as assessed by staining with the Hematocolor Set (Merck Millipore) followed by light microscopy analysis. Human neutrophils were isolated from healthy individuals and ADOA patients’ blood21. Briefly, cell separation was performed by Ficoll-Hypaque centrifugation (Pancoll human from PANTM BioTech). The granulocyte fraction was depleted of erythrocytes by lysis with a buffer containing NH4Cl, KHCO3 and EDTA. The resulting cell populations contained >95% neutrophils as assessed by Diff-Quik staining and light microscopy. Written, informed consent was obtained from all blood donors, and the Ethics Committee of the Canton Bern approved the study.
Human and mouse neutrophils were seeded on 12-mm glass cover slips in X-VIVOTM 15 medium (Lonza, Walkersville, MD, USA), primed with 25 ng per ml GM-CSF for 20 min, and subsequently stimulated with 10−8 M C5a, 100 ng per ml LPS or co-cultured with Escherichia (E.) coli GFP M655 (E. coli-GFP; a kind gift of E. Slack, ETH Zurich) for 15 min. In selected experiments, the following inhibitors were used 30 min before GM-CSF priming: Oligomycin A (2.5, 5, or 10 µg per ml), 2-DG (1, 3, 5 mM), rotenone (10 µM), antimycin A (5 µg per ml), Q-VD (20 µM), taxol (1 µM) and nocodazole (5 µM). In other experiments, cells were pre-cultured for 30 min in the presence of ATP (10, 100 µM, or 1 mM) or NMN (500 μM) and then stimulated for 45 min, besides GM-CSF/C5a, also with RNP-ICs-SLE [IgG, purified from with SLE antibody positive human plasma (Cat # DA1805, Trina Bioreactive AG, Nänikon, Switzerland, dilution 1:33), mixed with small nuclear ribonucleoprotein (SmRNP) (Cat # ATR01-02, Arotec, New Zealand, dilution 1:100)] or RNP-ICs-Ab [an anti-damaged DNA/RNA monoclonal antibody (clone 15A3, Cat # SMC-155, StressMarq Biosciences, Victoria, Canada, dilution 1:33) mixed with SmRNP]29 or P. aeruginosa (ATCC-BAA-47; strain HER-1018)42; with a multiplicity of infection of 10:1 (bacteria to neutrophils).
The human myeloid leukemia cell line PLB-985 originally from Collection of Microorganisms and Cell Cultures (DSMZ, Germany Cat # ACC 139), and was kindly provided by V. Witko-Sarsat, Cochin Institute, Paris, France, was cultured in RPMI 1640+ GlutaMAX medium supplemented with 10% FCS, 50 units per ml penicillin and 50 µg per ml streptomycin. Cell cultures were passaged twice or three times a week to maintain a cell density between 2 × 105 and 1 × 106 per ml. For granulocytic differentiation, PLB-985 cells were cultured at a density of 2 × 105 per ml in RPMI 1640 medium supplemented with 0.5% N,N-dimethylformamide (DMF), 1% Nutridoma-SP and 0.5% FCS53. The medium was changed once on day 3 during the differentiation period. On day 6, the cells were centrifuged and resuspended in fresh medium.
Down-regulation of mitochondria-shaping proteins by shRNA
Human and mouse short hairpin RNAs (shRNA) were subcloned in HIV-derived lentivirus vector pLKO.1-puromycin (human cells) or G418 (mouse cells) with a U6 promoter that had been purchased from Sigma-Aldrich. Lentiviral vector containing shRNA were calcium phosphate transfected together with a lentivirus envelope protein vector pMD2G and the packaging vector psPAX2 (provided by D. Trono, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland) into 293T/17 cells from American Type Culture Collection (ATCC, USA Cat # CRL-11268). Supernatants were collected after 24 and 48 h, cleared by low-speed centrifugation (425 × g for 10 min), filtered through a 0.22-μm filter (Merck Millipore), and the lentivirus vectors encoding human and mouse shRNAs added to human myeloid leukemia cell line (PLB-985) and mouse Hoxb8 cell lines20, respectively. pLKO.1 constructs encoding shRNAs against human and mouse were obtained from Sigma-Aldrich (Mission shRNA).
shRNA primer sequences: DRP1 (TRCN0000001097, CCG GGC TAC TTT ACT CCA ACT TAT TCT CGA GAA TAA GTT GGA GTA AAG TAG CTT TTT), mouse Drp1 (TRCN0000012605, CCG GGC TTC AGA TCA GAG AAC TTA TCT CGA GAT AAG TTC TCT GAT CTG AAG CTT TTT), human FIS1 (TRCN0000155375, CCG GCA AGA GCA CGC AGT TTG AGT ACT CGA GTA CTC AAA CTG CGT GCT CTT GTT TTT TG), mouse Fis1 (TRCN0000124386, CCG GCC TGG TTC GAA GCA AAT ACA ACT CGA GTT GTA TTT GCT TCG AAC CAG GTT TTT G), human MFN1 (TRCN0000051833, CCG GGC TCC CAT TAT GAT TCC AAT ACT CGA GTA TTG GAA TCA TAA TGG GAG CTT TTT G), mouse Mfn1 (TRCN0000081401, CCG GGC GTT TAA GCA GCA GTT TGT ACT CGA GTA CAA ACT GCT GCT TAA ACG CTT TTT G), human OPA1 (TRCN0000082846, CCG GCC GGA CCT TAG TGA ATA TAA ACT CGA GTT TAT ATT CAC TAA GGT CCG GTT TTT G), or mouse Opa1 (TRCN0000091111, CCG GCC GAC ACA AAG GAA ACT ATT TCT CGA GAA ATA GTT TCC TTT GTG TCG GTT TTT G).
The down-regulation of DRP1, FIS1, MFN1, and OPA1 after antibiotic selection was controlled by qPCR using primers as follows: human DRP1 (forward: 5′- TTC AAT CCG TGA TGA GTA TGC-3′, reverse 5′-TTA GAA GAG ACT GAT ACT GAG CA-3′), mouse Drp1 (forward: 5′-GAG TGT AAC TGA TTC AAT-3′, reverse 5′-GCA TAA GTA ACC TAT TCA-3′), human FIS1 (forward: 5′-AAG AGC ACG CAG TTT GAG-3′, reverse 5′-CAG GTA GAA GAC GTA ATC CC-3′), mouse Fis1 (forward: 5′-AGC GGG ACT ATG TCT TCT-3′, reverse 5′-GCA CAT ACT TTA GAG CCT TT-3′), human MFN1 (forward: 5′-GTT GGA GCG GAG ACT TAG-3′, reverse 5′-CGG ATT CTT ATA TGT TGC TTC AA-3′), mouse Mfn1 (forward: 5′-TAA TGG CAG AAA CGG TAT-3′, reverse 5′-TTC CTG TAT GTT GCT TCA-3′), or human OPA1 (forward: 5′-GGT TGT TGT GGT TGG AGA T-3′, reverse 5′-AGA GTC ACC TTA ACT GGA GAA-3′), mouse Opa1 (forward: 5′-AAG TGA CAA GCA TTA CAG G-3′, reverse 5′-CTC CAA GAT CCT CTG ATA CT-3′).
Reactive oxygen species measurements
ROS measurements in human and mouse neutrophils were performed using fluorescent detection of ROS activity by flow cytometer21. Neutrophils (2 × 106 per ml) were resuspended in X-VIVOTM 15 medium and neutrophils primed with GM-CSF and subsequently stimulated with C5a. For positive control experiments, we stimulated neutrophils with PMA (25 nM) in the absence of GM-CSF priming. DHR 123 was added to the cells at the final concentration of 1 μM. The reaction was stopped by adding 200 μl of ice-cold PBS, and the ROS activity of the samples was immediately measured by flow cytometry (BD FACSCalibur) and quantified using FlowJo software (Tree Star, Ashland, OR, USA).
Neutrophils were fixed with 4% paraformaldehyde for 10 min, washed with PBS, pH 7.4, and permeabilized using 0.05% saponin in PBS pH 7.4 for 2.5 min at room temperature. Subsequently, staining was performed in the presence of 0.05% saponin. Nonspecific binding was reduced by pre-incubation of the adherent cells with a blocking buffer (containing human immunoglobulins) at room temperature for 20 min. Indirect immunofluorescence staining was performed, using polyclonal goat anti-mouse neutrophil elastase (M-18, Cat # SC-9518, Santa Cruz Biotechnology, dilution 1:50) or polyclonal goat anti-mouse MPO (E-15, Cat # SC-34159, Santa Cruz Biotechnology, dilution 1:50) and rabbit anti-TFAM (Cat # NBP1-67995, Novus Biologicals, dilution 1:100) primary antibodies, diluted in blocking solution, and incubated overnight at 4 °C for staining tissue sections or 2 h for isolated neutrophils21. As staining controls, either mouse or rabbit control antibodies at the corresponding concentrations were employed. Appropriate Alexa Fluor® 488 (Cat # A11055, ThermoFischer Scientific, dilution 1:400) or Cyanine Cy™3 (Cat # 715-167-003, Jackson ImmunoResearch, dilution 1:100)-conjugated secondary antibodies were incubated at room temperature for 1 h. DNA was stained with PI (10 μg per ml) or monoclonal mouse anti-dsDNA (clone AE-2, Cat # MAB1293, Merck Millipore, dilution 1:200). Staining with cell-permeable fluorescent dyes, such as MitoSox Red (5 μM) or MitoTracker Orange (1 μM), was performed in live cells prior to fixation according to the corresponding manufacturers’ instructions. Samples were mounted in Prolong Gold mounting medium, image acquisition was performed using the confocal laser scanning microscope LSM 700 (Carl Zeiss Micro Imaging, Jena, Germany) with a ×63/1.40 oil DIC objective and analyzed with Imaris software (Bitplane AG, Zurich, Switzerland) as previously reported7,21.
Super-resolution multicolor imaging
High-resolution imaging was performed with a structured illumination microscope Elyra S.1 (Carl Zeiss) equipped with a Plan-Apochromat ×63/1.4 NA objective and an iXon 885 EM-CCD camera (Andor Technology), and was piloted with the ZEN Blue 2010 D software (Carl Zeiss). Image reconstruction was carried out with the Zen software (Carl Zeiss).
Quantification of released dsDNA in culture supernatants
Neutrophils (2 × 106 neutrophils per 500 μl of X-VIVO™ 15 medium) were stimulated as described above. At the end of the incubation period, a low concentration of DNase I (2.5 U per ml; Worthington Biochemical Corporation, Lakewood, NJ, USA) was added for an additional 10 min. Reactions were stopped by addition of 2.5 mM EDTA, pH 8.0. Cells were centrifuged at 200 × g for 5 min. One hundred microliters supernatant were then transferred to black, glass-bottom 96-well plates (Greiner Bio-One GmbH) and the fluorescent activity of PicoGreen dye bound to dsDNA was excited at 502 nm. The fluorescence emission intensity was measured at 523 nm using a spectrofluorimeter (SpectraMax M2, Molecular Devices, Biberach an der Riß, Germany), according to the instructions described in the Quant-iTTMPicoGreen® assay kit21.
Neutrophils were seeded on glass coverslips and stimulated as described above. Samples were fixed in mixture of 4% formaldehyde, and 0.1% glutaraldehyde in PBS (pH 7.4), and permeabilized first with 0.5% Triton X-100 for 15 min, followed by a 15-min incubation with 0.5% sodium dodecyl sulphate (SDS) at room temperature. Autofluorescence was then quenched with 0.5 mg per ml sodium borohydride (NaBH4) in PBS (pH 7.4) for 10 min on ice. Nonspecific binding to the Fc-receptor was blocked with a blocking buffer [7.5% BSA, 30% human IgG polyvalent (IVIG), and 30% normal goat sera (matching species with secondary antibody) and 1% ChromPure human IgG in PBS] at room temperature for 1 h. Anti-α-tubulin monoclonal antibody (clone DM1A, Cat # T9026, Sigma-Aldrich, dilution 1:100,000) was diluted in blocking buffer and incubated overnight at 4 °C, subsequently washed three times in PBS (pH 7.4) with 0.025% Triton X-100, and then further incubated with the secondary antibody, Alexa-555 or -488 goat anti-mouse IgG (Cat # A32727 or A32723, ThermoFischer Scientific, dilution 1:1000) diluted in 7.5% BSA plus 0.025% Triton X-100 at room temperature for 1 h. Following extensive washing with PBS (pH 7.4) plus 0.025% Triton X-100, the samples were counterstained with 1 μg per ml Hoechst 33342 at room temperature for 5 min in the dark. After several washes, the samples were mounted on glass slides using Prolong Gold mounting medium19,54. Images were acquired using the LSM 700. Ten representative pictures from each condition, containing more than 20 cells, were subjected to automated analysis to measure fluorescence mean intensity of anti-α-tubulin before and after the activation period using automated isosurface module of Imaris software.
F-actin staining and quantification
Neutrophils were seeded on 12-mm glass cover slips and treated as described above. Staining with MitoTracker Orange (1 μM), was performed in live cells prior to fixation. Cells were fixed with 4% paraformaldehyde for 5 min, subsequently washed three times in PBS (pH 7.4), and permeabilized with 0.5% saponin in buffer A (1% BSA in PBS) for 5 min at room temperature. F-actin was stained using 1.25% solution (7 μM) of Alexa Fluor® 488 Phalloidin (green) in PBS (pH 7.4) plus 0.05% BSA and 0.5% of saponin for 15 min at room temperature, protected from light. Cells were washed two times in PBS (pH 7.4) and counterstained with 1 μg per ml Hoechst 33342 to visualize the nuclei. After washing three times in PBS (pH 7.4), samples were mounted in Prolong Gold mounting media. Slides were examined and images acquired by confocal laser scanning microscopy (LSM 700). Ten representative pictures from each condition, containing more than 20 cells, were subjected to automated analysis to measure fluorescence mean intensity of F-actin before and after the activation period, using the automated isosurface module of Imaris software.
Analysis of mitochondrial localization
Imaris software was employed for quantification of mitochondrial localization. MitoTracker-stained mitochondria were imaged with an LSM 700 confocal microscope and processed using measurement point module and tab Intensity tool within Imaris software to quantitatively analyze images. Ten representative pictures from each condition, containing more than 20 cells were used to determine the localization of mitochondria with respect to their distance from the plasma membrane55.
Cell lysates of primary bone marrow-derived mouse or human blood neutrophils were prepared by lysing the cells with lysis buffer containing 50 mM Tris pH 7.4, 150 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, 10 mM sodium pyrophosphate, 50 mM sodium fluoride, 200 μM Na3VO4 and 1 mM PMSF in H2O. Shortly before use, a protease inhibitor cocktail was added to the lysis buffer. Cells were lysed for 25 min on ice, with frequent vortexing. After the lysis, supernatants were collected following high-speed centrifugation (10 min, 17,949 × g, 4 °C). In all, 30–50 μg of protein was loaded on 12% SDS polyacrylamide gels (Expedeon, Lucerna-Chem). The samples were separated by electrophoresis under reducing conditions and transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon-P; Merck Millipore). Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline solution containing 0.1% Tween 20 (TBST) for 1 h, followed by incubation with monoclonal anti-OPA1 antibody (clone 18/OPA1, Cat # 612606, BD Biosciences, dilution 1:1000) in TBST and 5% non-fat dry milk at 4 °C overnight. Membranes were washed using TBST, then incubated with the corresponding HRP-conjugated secondary antibody (Cat # GENA931, Sigma-Aldrich, dilution 1:10,000) and visualized by chemiluminescence (ECL Western Blotting Analysis System; GE Healthcare or Luminata Forte; Merck Millipore).
E. coli-GFP (25 × 106 per ml) were opsonized with 2% mouse serum in 1× Hank’s Balanced Salt Solution (HBSS; LuBioScience GmbH) for 15 min (rotating end-over-end, 37 °C). Primary bone marrow-derived mouse neutrophils (0.5 × 106) were resuspended in 200 μl of X-VIVOTM medium without phenol red or antibiotics and 200 μl of opsonized bacteria was then added to the cells. The cells were incubated with the bacteria for 35 min (rotating end-over-end, 37 °C). Phagocytosis was stopped by adding 400 μl of ice-cold PBS with 0.02% EDTA to the cells. After one washing step with 400 μl of ice-cold PBS, the cells were analyzed by flow cytometer (BD FACSCalibur).
Bacterial killing assay
A single colony of E. coli-GFP was cultured in Luria broth base (LB) medium (Sigma-Aldrich) or a colony of P. aeruginosa was cultured in tryptic soy broth at 37 °C, shaking, overnight. The bacterial cultures were diluted 1:100 in LB medium, grown to mid-logarithmic growth phase (OD600 = 0.7) and centrifuged at 1000 × g for 5 min. Bacterial pellets were washed twice with 1× Hank’s Balanced Salt Solution (HBSS; LuBioScience GmbH) and again gently centrifuged at 100 × g for 5 min to remove any clumped bacteria. Bacteria were opsonized with 10% mouse sera (heat-inactivated) in 1× HBSS, rotating end-over-end at 37 °C for 20 min, and then used immediately. Activated neutrophils (1 × 107 per ml in RPMI 1640 plus 2% FCS in absence of antibiotics) were mixed with an equal volume of opsonized bacteria (5 × 107 per ml) at 1:5 ratios, in the presence or absence of 100 U per ml DNase I (Worthington Biochemical Corporation, Lakewood, NJ, USA). The co-cultures of cells and bacteria were rotated end-over-end at 37 °C for 30 min. At the end of the incubation period, an equal volume of ice-cold 1× HBSS was added to each tube to stop the reaction, and cells were pelleted by gentle centrifugation (5 min, 100 × g, 4 °C) using a swing-out rotor. Supernatants containing bacteria were collected, diluted 1:300, plated on agar, and grown overnight before counting the colonies. The tubes containing bacteria alone were treated the same way and used as controls21.
For each experiment, P. aeruginosa inoculum was freshly prepared from a frozen glycerol stock and grown overnight on tryptic soy agar (TSA)56. The inoculum was washed in PBS, and the concentration was adjusted by spectrophotometry. The actual CFU in the inoculum was determined each time by plating serial dilutions on TSA. Mice were inoculated with 2 × 106 CFU per mouse.
In vivo lung infection
Groups of mice were sedated with 100 mg per kg ketamine and 10 mg per kg xylazine and intranasally inoculated by applying 10 μl of inoculum onto each nare. Mice were killed after 18 h for tissue harvest. The dissected spleen and right lung were homogenized, serially diluted in 1% protease peptone, and plated in duplicate on TSA. Aliquots of lung homogenates were stored at −80 °C until further analysis. Left lungs were fixed in formalin solution and processed for immunofluorescence staining. Lung sections were stained using goat anti-mouse MPO antibody (E-15, Cat # SC-34159, Santa Cruz Biotechnology, dilution 1:50) at 4 °C overnight, followed by conjugated Alexa Fluor 488 donkey anti-goat IgG (Cat # A11055, ThermoFischer Scientific, dilution 1:100) at room temperature for 1 h, and subsequently with 10 μg per ml PI for 10 min.
MPO activity assay
MPO activity was measured in triplicate using a spectrophotometric assay in 50 mM potassium phosphate, pH 6, containing 0.167 mg per ml o-dianisidine dihydrochloride and 0.0005% hydrogen peroxide.
Measurement of mitochondrial DNA copy number
Total genomic DNA was extracted from primary mouse or human neutrophils with a NucleoSpin® tissue kit. Quantitative PCR (qPCR) assays were performed using iQ SYBR Green Supermix (Bio-Rad Laboratories). Mouse primers57 were as follows: Mouse cytochrome c oxidase subunit 1 (mt-Co1) (forward: 5′-CCA GTG CTA GCC GCA GGC AT-3′, reverse 5′- TCT GGG TGC CCA AAG AAT CAG AAC A-3′) and for nDNA primers mouse β2 microglobulin (mB2M1) (forward: 5′- ATG GGA AGC CGA ACA TAC TG-3′, reverse 5′-CAG TCT CAG TGG GGG TGA AT-3′). Human primers25 were as follows: human D-Loop (forward: 5′-CAT CTG GTT CCT ACT TCA GGG-3′, reverse 5′-CCG TGA GTG GTT AAT AGG GTG-3′) and human ATP synthase protein 8 (MT-ATP8) (forward: 5′-ATG GCC CAC CAT AAT TAC CC-3′, reverse 5′-CAT TTT GGT TCT CAG GGT TTG-3′), and human β2 microglobulin (B2M) (forward: 5′-TGC TGT CTC CAT GTT TGA TGT ATC T-3′, reverse 5′-TCT CTG CTC CCC ACC TCT AAG T-3′). Primers were synthesized by Microsynth AG. Duplicate real-time PCRs were performed in the iQ5 Multicolor Real-time PCR Detection System (Bio-Rad Laboratories), with 25 ng extracted genomic DNA, and 200 nM of each primer in 25 µl final PCR mix. The cycling variables were 3 min at 95 °C followed by 40 cycles of 10 s at 95 °C and 30 s at 55 °C.
Enzyme assays of mitochondrial respiratory chain complexes I and III
Mitochondria from differentiated Hoxb8 mouse neutrophils were prepared using the Mitochondria Isolation Kit according to manufacturer’s protocol (Thermo Fisher Scientific). Isolated mitochondria were resuspended in 10 mM ice-cold hypotonic Tris buffer (pH 7.6) and stored at −80 °C. Before use, mitochondria were subjected to three cycles of sonication to disrupt the mitochondrial membrane. The enzymatic activities of CI, CIII and citrate synthase were determined spectrophotometrically as described58 in 10–20 μg extracted protein using 1 ml sample cuvettes at 30 °C. CI activity was measured as the rotenone-sensitive NADH:decylubiquinone oxidoreductase reaction and the decrease in absorbance of NADH oxidation was read at 340 nm. CIII function was measured as the antimycin A-sensitive decylubiquinol:cytochrome c reductase reaction and increasing reduction of cytochrome c was read at 550 nm. Citrate synthase was measured as the rate of production of coenzyme A from oxaloacetate by measuring the reduction of 5,5′-dithiobis-2-nitrobenzoic acid at 412 nm. The values were normalized to the amount of protein in each sample. Enzymatic CI and CIII activities were calculated as ratios to the mitochondrial marker enzyme citrate synthase (mU per mU citrate synthase).
Extracellular flux analysis using Seahorse XF24
OCR and ECAR were investigated simultaneously using a Seahorse XF24 bioanalyzer (Agilent Technologies, CA, USA). Primary mouse neutrophils were seeded in unbuffered culture medium at 480,000 per well in polylysine-coated XF24-well assay plates. After the initial assessment of basal OCR and ECAR rates, sequential exposures to modulators of mitochondrial function were injected at optimized concentrations using a standard mitochondrial stress test paradigm. First, mitochondrial phosphorylation was inhibited by adding oligomycin (2 µM). Next, the mitochondrial electron transport chain was stimulated maximally by the addition of the uncoupler carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (1.5 µM, FCCP). Finally, mitochondrial respiration was inhibited at the level of complex I by addition of rotenone (1 µM), and glycolysis inhibited by injection of 2-deoxy-D-glucose (50 mM, 2-DG). Steady-states were calculated as the mean of three OCR measurement cycles. After each experiment, OCR and ECAR data were normalized to corresponding cell numbers in each well using protein determination.
ATP/ADP, NAD+/NADH, and lactate measurements
Resting and activated mouse neutrophils (4 × 106 per ml) were treated with and without glycolysis (2-DG) or CI (rotenone) inhibitors for 70 min at 37oC in DMEM medium (no glucose, no glutamine, no phenol red (Cat # A14430-01, Thermo Fisher Scientific). 12 mM D-glucose was added for lactate and ATP/ADP measurements. For NAD+/NADH quantitative analysis, cells were treated as described above, but cultured in PBS. Upon activation, cells were placed on ice for lactate analysis or the reaction was stopped by adding Krebs-Ringer bicarbonate buffer (Cat # K4002, Sigma-Aldrich) and cell pellets were resuspended in Krebs-Ringer bicarbonate buffer with 1% TCA (for ATP/ADP) or cold PBS (for NAD+/NADH). The ATP/ADP ratio was measured by Cellular ADP/ATP‐Glo™ Assay (Promega AG), according to the instructions of the manufacturer. Lactate and the NAD+/NADH ratio were measured for luciferase activity by Lactate-Glo™ Assay or NAD/NADH‐Glo™ Assay (Promega AG), according to the instructions of the manufacturer. Values were normalized to the total protein content.
Primary bone marrow-derived mouse neutrophils (4 × 106 cells per ml) were resuspended in X-VIVOTM 15 medium, primed with GM-CSF and subsequently activated with C5a. For degranulation analysis of azurophil granule protein, in the final 5 min of GM-CSF priming, cytochalasin B (5 µM) was added to the cell suspension. Degranulation of azurophil granules was determined by measuring the increase in CD63 expression as a surrogate marker. Surface expression of CD63 was performed using PE-conjugated rat anti-mouse CD63 monoclonal antibody (clone NVG-2; Cat # 564222, BD Biosciences, dilution 1:50) and flow cytometry (FACSVerse, BD Biosciences)59. CD63 expression was quantified using FlowJo software (Tree Star, Ashland, OR, USA). For β-glucosaminidase activity, the reaction was stopped by adding ice-cold HBSS-0.1% BSA and the supernatants were collected following a centrifugation step (20 min, 425 × g, 4 °C). Cell pellets were lysed with 0.12% Triton X-100 for 10 min at room temperature. The substrate solution (5 mM p-nitrophenyl-2-acetamido-2-deoxy-α-d-glucopyranoside in 25 mM sodium citrate, pH 4.5) was added to the cell lysate and supernatant followed by incubation at 37 °C for 1 h. The reaction was stopped by adding 52 mM glycine-NaOH, pH 10.5, and the absorbance measured at 410 nm using a SpectraMax M2 plate reader (Bucher Biotech). The amount of β-glucosaminidase released in supernatants of neutrophils was expressed as a percentage of the total β-glucosaminidase60.
Transmission electron microscopy
Neutrophils were centrifuged at 425 × g and 4oC for 20 min. The supernatants were removed and 1 ml cold 2.5% glutaraldehyde solution in 0.15 M HEPES was added to cell pellets. Electron microscopy images were taken at an acceleration voltage of 80 keV on a Philips CM100 electron microscope equipped with a FEI Morgagni digital camera and images analyzed using iTEM software. The numbers of mitochondria per cell and the morphometric analysis of mitochondria and cristae were carried out as previously described using the Imaris software10,35,61.
Cell death assay
Analysis of all data was performed using the Prism software (GraphPad Software Inc., San Diego, CA, USA). All data are expressed as the means ± SEM. To compare groups, one-way ANOVA followed by Tukey’s multiple comparisons test or the Kruskal–Wallis test was applied. Differences between many groups at two levels were tested by two-way ANOVA followed by Bonferroni post tests. p values of <0.05 were considered statistically significant.
All relevant data are available from the authors upon request.
Brinkmann, V. et al. Neutrophil extracellular traps kill bacteria. Science 303, 1532–1535 (2004).
Yousefi, S., Mihalache, C., Kozlowski, E., Schmid, I. & Simon, H. U. Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps. Cell Death Differ. 16, 1438–1444 (2009).
Simon, D., Simon, H. U. & Yousefi, S. Extracellular DNA traps in allergic, infectious, and autoimmune diseases. Allergy 68, 409–416 (2013).
Garcia-Romo, G. S. et al. Netting neutrophils are major inducers of type I IFN production in pediatric systemic lupus erythematosus. Sci. Transl Med. 3, 73ra20 (2011).
Lande, R. et al. Neutrophils activate plasmacytoid dendritic cells by releasing self-DNA-peptide complexes in systemic lupus erythematosus. Sci. Transl Med. 3, 73ra19 (2011).
Yousefi, S. et al. Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defence. Nat. Med. 14, 949–953 (2008).
Morshed, M. et al. NADPH oxidase–independent formation of extracellular DNA traps by basophils. J. Immunol. 192, 5314–5323 (2014).
Shaw, J. M. & Nunnari, J. Mitochondrial dynamics and division in budding yeast. Trends Cell Biol. 12, 178–184 (2002).
Cipolat, S., Martins de Brito, O., Dal Zilio, B. & Scorrano, L. Opa1 requires mitofusin1 to promote mitochondrial fusion. Proc. Natl Acad. Sci. USA 101, 15927–15932 (2004).
Frezza, C. et al. OPA1 controls apoptotic cristae remodeling independently from mitochondrial fusion. Cell 126, 177–189 (2006).
Cipolat, S. et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cell 126, 163–175 (2006).
Han, J. et al. OPA1 mutations and mitochondrial DNA haplotypes in autosomal dominant optic atrophy. Genet. Med. 8, 217–225 (2006).
Chen, H., Chomyn, A. & Chan, D. C. Disruption of fusion results in mitochondrial heterogeneity and dysfunction. J. Biol. Chem. 280, 26185–26192 (2005).
Zorzano, A., Liesa, M., Sebastián, D., Segalés, J. & Palacín, M. Mitochondrial fusion proteins: dual regulators of morphology and metabolism. Sem. Cell Dev. Biol. 21, 566–574 (2010).
Alexander, C. et al. OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28. Nat. Genet. 26, 211–215 (2000).
Yu-Wai-Man, P. et al. Multi-system neurological disease is common in patients with OPA1 mutations. Brain 133, 771–786 (2010).
Archer, S. L. Mitochondria dynamics-mitochondria fission and fusion in human diseases. N. Engl. J. Med. 369, 2236–2251 (2013).
Maianski, N. A. et al. Functional characterization of mitochondria in neutrophils: a role restricted to apoptosis. Cell Death Differ. 11, 143–153 (2004).
Stojkov, D. et al. ROS and glutathionylation balance cytoskeletal dynamics in neutrophil extracellular trap formation. J. Cell. Biol. 216, 4073–4090 (2017).
Rozman, S. et al. The generation of neutrophils in the bone marrow is controlled by autophagy. Cell Death Differ. 22, 445–456 (2015).
Amini, P. et al. NET formation can occur independently of RIPK3 and MLKL signaling. Eur. J. Immunol. 46, 178–184 (2016).
Schimpf, S., Schaich, S. & Wissinger, B. Activation of cryptic splice sites is a frequent splicing defect mechanism caused by mutations in exon and intron sequences of the OPA1 gene. Hum. Genet. 118, 767–771 (2006).
Chen, H. et al. Mitochondrial fusion is required for mtDNA stability in skeletal muscle and tolerance of mtDNA mutations. Cell 141, 280–289 (2010).
Amati-Bonneau, P. et al. OPA1 mutations induce mitochondrial DNA instability and optic atrophy ‘plus’ phenotypes. Brain 131, 338–351 (2008).
Jackson, C. B., Zbinden, C., Gallati, S. & Schaller, A. Heterologous expression from the human D-loop in organelle. Mitochondrion 17, 67–75 (2014).
Sitarz, K. S. et al. Opa1 mutations induce mtDNA proliferation in leukocytes of patients with dominant optic atrophy. Neurology 79, 1515–1517 (2012).
Amati-Bonneaua, P. et al. OPA1-associated disorders: phenotypes and pathophysiology. Biochem. Cell. Biol. 41, 1855–1865 (2009).
Alavi, M. V. et al. A splice site mutation in the murine Opa1 gene features pathology of autosomal dominant optic atrophy. Brain 130, 1029–1042 (2007).
Lood, C. et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat. Med. 22, 146–153 (2016).
Amulic, B., Cazalet, C., Hayes, G. L., Metzler, K. D. & Zychlinsky, A. Neutrophil function: from mechanisms to disease. Annu. Rev. Immunol. 30, 459–489 (2012).
Gray, J. J. et al. N-terminal cleavage of the mitochondrial fusion GTPase OPA1 occurs via a caspase-independent mechanism in cerebellar granule neurons exposed to oxidative or nitrosative stress. Brain Res. 12, 28–43 (2013).
Mollinedo, F., Nieto, J. M. & Andreu, J. M. Cytoplasmic microtubules in human neutrophil degranulation: Reversible inhibition by the colchicine analogue 2-methoxy-5-(2’,3’,4’trimethoxyphenyl)-2,4,6-cycloheptatrien-1-one. Mol. Pharmacol. 36, 547–555 (1989).
Xu, J., Wang, F., Van Keymeulen, A., Rentel, M. & Bourne, H. R. Neutrophil microtubules suppress polarity and enhance directional migration. Proc. Natl Acad. Sci. USA 102, 6884–6889 (2005).
Parker, H., Dragunow, M., Hampton, M. B., Kettle, A. J. & Winterbourn, C. C. Requirements for NADPH oxidase and myeloperoxidase in neutrophil extracellular trap formation differ depending on the stimulus. J. Leukoc. Biol. 92, 841–849 (2012).
Cogliati, S. et al. Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency. Cell 155, 160–171 (2013).
Lodi, R. et al. Defective mitochondrial adenosine triphosphate production in skeletal muscle from patients with dominant optic atrophy due to OPA1 mutation. Arch. Neurol. 68, 67–73 (2011).
Bershadsky, A. D. & Gelfand, V. I. ATP-dependent regulation of cytoplasmic microtubule disassembly. Proc. Natl Acad. Sci. USA 78, 3610–3613 (1981).
Borregaard, N. & Herlin, T. Energy metabolism of human neutrophils during phagocytosis. J. Clin. Invest. 70, 550–557 (1982).
Ying, W. NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences. Antioxid. Redox Signal. 10, 179–206 (2008).
Stein, L. R. & Imai, S. I. The dynamic regulation of NAD metabolism in mitochondria. Trends Endocrinol. Metabol. 23, 420–428 (2012).
van Raam, B. J. et al. Mitochondrial membrane potential in human neutrophils is maintained by complex III activity in the absence of supercomplex organization. PLoS ONE 3, e2013 (2008).
Benarafa, C., Priebe, G. P. & Remold-O’Donnell, E. The neutrophil serine protease inhibitor Serpinb1 preserves lung defense functions in Pseudomonas aeruginosa infection. J. Exp. Med. 204, 1901–1909 (2007).
Rehm, S. R., Gross, G. N. & Pierce, A. K. Early bacterial clearance from murine lungs. Species-dependent phagocyte response. J. Clin. Invest. 66, 194–199 (1980).
Nathan, C. Neutrophils and immunity: challenges and opportunities. Nat. Rev. Immunol. 6, 173–182 (2006).
Yousefi, S. & Simon, H. U. NETosis – does it really represent nature’s “suicide bomber”? Front. Immunol. 7, 328 (2016).
Zmijewski, J. W. et al. Mitochondrial respiratory complex I regulates neutrophil activation and severity of lung injury. Am. J. Respir. Crit. Care. Med. 178, 168–179 (2008).
Bao, Y. et al. Mitochondria regulate neutrophil activation by generating ATP for autocrine purinergic signaling. J. Biol. Chem. 289, 26794–26803 (2014).
Wang, H., Li, T., Chen, S., Gu, Y. & Ye, S. Neutrophil extracellular trap mitochondrial DNA and its autoantibody in systemic lupus erythematosus and a proof-of-concept trial of metformin. Arthritis Rheumatol. 67, 3190–3200 (2015).
Zhang, Q. et al. Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature 464, 104–107 (2010).
Caielli, S. et al. Oxidized mitochondrial nucleoids released by neutrophils drive type I interferon production in human lupus. J. Exp. Med. 213, 697–713 (2016).
Duanmu, C., Lin, C. M. & Hamel, E. Tubulin polymerization with ATP is mediated through the exchangeable GTP site. Biochim. Biophys. Acta 881, 113–123 (1986).
Oropesa, M. et al. Apoptotic microtubule network organization and maintenance depend on high cellular ATP levels and energized mitochondria. Apoptosis 16, 404–424 (2011).
Pedruzzi, E., Fay, M., Elbim, C., Gaudry, M. & Gougerot-Pocidalo, M. A. Differentiation of PLB-985 myeloid cells into mature neutrophils, shown by degranulation of terminally differentiated compartments in response to N-formyl peptide and priming of superoxide anion production by granulocyte–macrophage colony-stimulating factor. Br. J. Haematol. 117, 719–726 (2002).
Feltrin, D. & Pertz, O. Assessment of Rho GTPase signaling during neurite outgrowth. Methods Mol. Biol. 827, 181–194 (2012).
Quintana, A. et al. Sustained activity of calcium release-activated calcium channels requires translocation of mitochondria to the plasma membrane. J. Biol. Chem. 281, 40302–40309 (2006).
Basilico, P., Cremona, T. P., Oevermann, A., Piersigilli, A. & Benarafa, C. Increased myeloid cell production and lung bacterial clearance in mice exposed to cigarette smoke. Am. J. Respir. Cell Mol. Biol. 54, 424–435 (2016).
Tan, A. S. et al. Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA. Cell. Metab. 21, 81–94 (2015).
Schaller, A. et al. Molecular and biochemical characterization of a novel mutation in POLG associated with Alpers syndrome. BMC Neurol. 11, 4 (2011).
Uriarte, S. M. et al. Granule exocytosis contributes to priming and activation of neutrophil respiratory burst. J. Immunol. 187, 391–400 (2011).
Sato, T., Hongu, T., Sakamoto, M., Funakoshi, Y. & Kanaho, Y. Molecular mechanisms of N formyl-methionyl-leucyl-phenylalanine - induced superoxide generation and degranulation in mouse neutrophils: phospholipase D is dispensable. Mol. Cell. Biol. 33, 136–145 (2013).
Gomes, L. C., Benedetto, G. D. & Scorrano, L. During autophagy mitochondria elongate, are spared from degradation and sustain cell viability. Nat. Cell Biol. 13, 589–600 (2011).
Wang, X., He, Z., Liu, H., Yousefi, S. & Simon, H. U. Neutrophil necroptosis is triggered by ligation of adhesion molecules following GM-CSF priming. J. Immunol. 197, 4090–4100 (2016).
This work was supported by grants from the Swiss National Science Foundation to S.Y. (number 31003A_173215) and H.-U.S. (number 310030_166473). P.A. and D.S. are Ph.D. students of the Graduate School of Cellular and Biomedical Sciences of the University of Bern. Images were acquired on equipment supported by the Microscopy Imaging Centre of the University of Bern.
The authors declare no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Amini, P., Stojkov, D., Felser, A. et al. Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production. Nat Commun 9, 2958 (2018). https://doi.org/10.1038/s41467-018-05387-y
Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects
Developmental & Comparative Immunology (2021)
Cells Lacking PA200 Adapt to Mitochondrial Dysfunction by Enhancing Glycolysis via Distinct Opa1 Processing
International Journal of Molecular Sciences (2021)
Metabolic activation and colitis pathogenesis is prevented by lymphotoxin β receptor expression in neutrophils
Mucosal Immunology (2021)
High-mobility group box protein-1 induces acute pancreatitis through activation of neutrophil extracellular trap and subsequent production of IL-1β
Life Sciences (2021)