Fig. 4 | Nature Communications

Fig. 4

From: Cryopreservation of infectious Cryptosporidium parvum oocysts

Fig. 4

Response of C. parvum to dehydration and CPA uptake prior to freezing. The shrink-swell response of bleached oocysts to a challenge with 1 M trehalose for 10 min, with or without subsequent addition of DMSO for 0 min or 20 min (at final concentration of 0.5 M trehalose/30% DMSO) was measured by image analysis and compared to the PBS control. Loss of 57.2 ± 5.2% of oocyst volume related to dehydration in trehalose is partly restored by addition of DMSO. This is likely related to a decreased trehalose gradient after immediate addition of DMSO (0 min) or intra-oocyst uptake of DMSO during 20 min incubation. Values indicate means and error bars indicate standard deviation (n = 3). DIC micrographs demonstrate volumetric changes of oocysts. Scale indicates 5 µm

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