Fig. 2 | Nature Communications

Fig. 2

From: Cryopreservation of infectious Cryptosporidium parvum oocysts

Fig. 2

Vitrified C. parvum oocysts are viable and excyst. a To ensure vitrification, bleached oocysts were dehydrated in 1 M trehalose and then suspended in 0.5 M trehalose/30% DMSO. Oocysts were then immediately loaded into microcapillaries and plunged into liquid nitrogen for 5 min, or incubated with CPA solution for 20 min followed by loading of microcapillaries and freezing. Oocysts were thawed by quickly transferring the microcapillary from liquid nitrogen to a 37 °C water bath. Viability was quantified after CPA removal based on PI exclusion and quality of excysted sporozoites. b Oocyst viability was determined by PI exclusion, both pre-freeze and after cryogenic storage. Values indicate means and error bars indicate standard deviation (n = 12). c DIC micrographs show excysted sporozoites. Sporozoite viability was assessed on basis of their shape, structure and observed motility after excystation in 0.75% taurocholic acid at 37 °C. Scale indicates 5 µm

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