Fig. 4 | Nature Communications

Fig. 4

From: The concerted roles of FANCM and Rad52 in the protection of common fragile sites

Fig. 4

FANCM is important for suppressing Ras-induced CFS expression and mitotic recombination at Flex1. ac Frequency of CFS expression at FRA16D in HCT116 cells was determined after expressing H-Ras V12 (Ras) or vector (a), Ras along with FANCM shRNA or control vector (b), or Ras and Flag-FANCM (WT or K117R) with endogenous FANCM silenced by shRNA (c). Expression of Ras and FANCM is shown by western blot analysis. d, f, g Spontaneous recombination was determined in U2OS (HR-Flex) cells with p53 silenced by shRNA (to prevent Ras-induced senescence) 1 week after Ras expression (d), Ras expression following expression of indicated shRNAs or vector (f), or Ras and Flag-FANCM WT or K117R with endogenous FANCM silenced by shRNA (g). Indicated protein expression is shown by western blot analysis. e ChIP analysis of γH2AX at Flex1 was performed in U2OS cells containing pCEP4-Flex1 or pCEP4-Luc plasmids 72 h after Ras overexpression

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