Fig. 1 | Nature Communications

Fig. 1

From: miR-103 promotes endothelial maladaptation by targeting lncWDR59

Fig. 1

LncRNAs upregulated in EC-Dicerflox mice during atherosclerosis and targets of let-7b and miR-103. a Pie graph from genome-wide microarray analysis for lncRNA genes differentially expressed in the aortas of EC-Dicerflox mice compared with EC-DicerWT mice fed 12 weeks of HFD (n = 2 mice per group). Not-regulated lncRNA genes are in green, and modulated lncRNAs are in gray. Among these, up- and downregulated lncRNAs with a P value cutoff of 0.05 were divided according to their fold change. b Pie graph representing the 97 significantly upregulated lncRNAs, following RNA-seq analysis from MAoECs: 53 unknown lncRNAs (green), 5 lncRNAs for which the sequence was discovered by RNA-seq or RACE-PCR (blue), and 39 lncRNAs with a sequence annotated in NONCODE or ENSEMBL databases (gray). Three out of 36 lncRNAs showed a known function (pink). c Gene locus and full transcript sequence for 2 new lncRNAs, upregulated in EC-Dicerflox mice, i.e., Leonardo and lncWDR59. Detected regions for Leonardo (792 bp) and lncWDR59 (1.61 kb) sequences derive from RNA-seq or RACE-PCR analysis, respectively. Probes from genome-wide microarray are reported in red. d Cytoscape interaction network from RNAHybrid binding sites prediction between downregulated miRNAs and upregulated lncRNAs with a determined sequence in EC-Dicerflox mice. LncRNA targets of let-7b or miR-103 are in light blue and red, respectively. LncRNAs with BS for both let-7b and miR-103 are in orange. In gray are the rest of miRNAs target lncRNAs lacking a BS for miR-103 or let-7b. e, f The in vivo upregulation of lncRNAs with BS for let-7b and miR-103 was confirmed in EC-Dicerflox mice (n = 4–7 mice per group) (e) and in MAoECs after let-7b or miR-103 inhibition (f) by qPCR (n = 6 per group). FC > 2. g Fold enrichment of lncRNA transcripts in the RISC complex after transfection of MAoECs with let-7b or miR-103 mimics together with a mutant form of Ago2, following an immunoprecipitation (GW182-IP), measured by qPCR. Results of three independent experiments are shown. GAPDH and B2M were used as housekeeping genes and relative expression analysis. FC fold change of control (ctrl) group. The data are represented as mean ± SEM of the indicated number (n) of repeats. *P < 0.05, and **P < 0.01 by Student’s t-test

Back to article page