Fig. 1 | Nature Communications

Fig. 1

From: Embryos and embryonic stem cells from the white rhinoceros

Fig. 1

Operational setting of the transrectal oocyte retrieval. a An anaesthetized white rhinoceros positioned in sternal recumbency during oocyte collection. One operator guides the OPU instrument inside the rectum directly to the stimulated ovary. The second operator injects the needle into the follicles under ultrasound guidance and performs the aspiration and repeatedly flushing the follicle up to ten times. The third operator replaces the full sterile 50-ml falcon tubes with an empty one. The 3D tomographic ultrasound image (b) generated with the portable 3D/4D ultrasound unit Voluson I (GE Healthcare, Solingen, Germany) shows different slices of the ovarian parenchyma which contain one central echogenic corpus luteum (white asterisk) and many anechoic follicles ranging from 4 to 20 mm in diameter. The 3D render mode ultrasound image (c) shows a virtual cut through the stimulated rhinoceros ovary. In this section eight different follicles are visible. d Ten rhinoceros oocytes after transrectal oocyte retrieval. Most of the oocytes collected had an intact cumulus oocyte complex besides the fact that the double-lumen aspiration needle used has a length of 950 mm and the vacuum applied was 100 mmHg. Bar 100 µm

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