Table 3 Summary of the fitness scores of E. coli tRNA family

From: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

  1. Highlighted are tRNAs not essential to E. coli in rich medium (light green for common tRNAs, deep green for a special tRNA encoded by selC to incorporate an unnatural amino acid) and tRNAs that we failed to design any sgRNAs for (gray). In addition, * the CAT anticodon is modified with a lysidine at C34 to recognize ATA codon of isoleucine, rather than methionine, # these two clusters of tRNA genes belongs to one tRNA isotype with CAT anticodon, UD undetermined (failure to design sgRNA)