Table 2 The phenotypes studied in this work

From: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

Phenotype Selective condition Control condition
Essentiality dCas9, LB Empty plasmid, LB
Auxotrophy MOPS LB
L-Trp biosynthesis 0.5 g/L casamino acid, MOPS LB
Furfural tolerance 0.4 g/L furfural, MOPS initial, see Supplementary Fig. 7
Isobutanol tolerance 4 g/L isobutanol, MOPS initial, see Supplementary Fig. 7