Fig. 6 | Nature Communications

Fig. 6

From: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

Fig. 6

Genes conferring tolerance phenotypes with respect to the toxic chemicals isobutanol (purple) and furfural (green). a, b Volcano plot of gene fitness in MOPS medium supplemented with 4 g/L isobutanol (a; FDR = 0.1, dashed line) and 0.4 g/L furfural (b; FDR = 0.05, dashed line). c Genomic plots of fitness for library variants in the presence of furfural (outer, green), isobutanol (middle, purple) and no supplementation (MOPS medium alone; inner, blue). The bars of each circle indicate the fitness for genes of relevant phenotypes. ncRNAs conferring significant phenotypes (‘+’ for tolerance and ‘−’ for sensitivity) (FDR < 0.05 or |fitness|>3 for auxotrophy in MOPS media and furfural tolerance, FDR < 0.1 or |fitness|>3 for isobutanol tolerance) under these conditions are highlighted

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