Fig. 4 | Nature Communications

Fig. 4

From: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

Fig. 4

CRISPRi screening maps ncRNA contributions to fitness. Volcano plot of ncRNA-coding gene fitness and −Log10P value of two-tailed MWU test. Dashed lines represent a threshold (FDR = 0.05) for calling hits based on the screening score (see Methods). Three groups of gene (each dot denotes a gene cluster with one or multiple genes, see Methods) are shown: purple, ribosomal RNAs; green, tRNAs; gray, other ncRNAs. Gene names for tRNAs that were not essential to E. coli identified by CRISPRi in rich medium and other ncRNAs reported to confer significant growth defects are given

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