Fig. 3 | Nature Communications

Fig. 3

From: Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP

Fig. 3

Concentration-response dependences of CNP and ANP signals. Responses to different ANP and CNP concentrations were recorded either using SICM/FRET (a, b) as described in Fig. 1, or by simple FRET imaging with global ligand application into the bath solution (c, d). In a, b, calculated peptide concentrations at the membrane are shown. 700, 210, and 70 nM were obtained by filling SICM pipetted with 100 µM, 30 µM, and 10 µM of CNP or ANP, respectively. Data for 700 nM are reproduced from Fig. 1 to facilitate the comparison. Data represent mean ± s.e.m. In a, b, numbers of cells/mice are shown above the bars. In d, 9–12 cells from three mice each were analysed for each data point. In c, data are reproduced from our previous study19 for comparison

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