Fig. 2 | Nature Communications

Fig. 2

From: Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP

Fig. 2

Control SICM/FRET experiments. Recordings were performed in presence of 100 µM IBMX (unselective PDE inhibitor, preincubated for 3–5 min in the bath solution) (a, b) or in NPR2 knockout myocytes (c, d) as described in Fig. 1. Representative scans and data quantifications are shown. These data rule out the possibility that local PDEs might affect the specific NPR1 localisation measured by SICM/FRET in Fig. 1e–g and confirm the specificity of the applied stimulation protocol for the studied receptors/ligands. Data represent mean ± s.e.m. values of the indicated number of cells/mice. *P < 0.01 (Kruskal–Wallis test)

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