Fig. 1 | Nature Communications

Fig. 1

From: Distinct submembrane localisation compartmentalises cardiac NPR1 and NPR2 signalling to cGMP

Fig. 1

Spatial distribution of NPR1 and NPR2 induced cGMP signals in cardiomyocytes. a, b Representative SICM image and corresponding FRET ratio traces recorded from whole single mouse ventricular cardiomyocytes expressing the cytosolic cGMP biosensor red cGES-DE5 after local NPR2 stimulation on the crest of the cell and in the T-tubule. The cell was superfused with buffer A, and NPR2 was locally stimulated from the scanning nanopipette filled with CNP (100 µM) by applying pressure (276 kPa). Estimated peptide concentration at the membrane was 700 nM (see Supplementary Fig. 3). c Quantification of the cGMP-FRET responses to CNP. d Spatio-temporal pattern of FRET response measured in different regions of the cell after CNP application to a single T-tubule, showing far-reaching cGMP signals. Scale bar, 10 µm. e, f Representative SICM image and corresponding FRET ratio traces after local NPR1 stimulation from the scanning nanopipette filled with 100 µM ANP. Quantification is in g. All data plotted as means ± s.e.m. Number of cells/mice is above the bars. Difference in c is not significant; in g, P < 0.01 by mixed ANOVA followed by Wald χ2-test. h Spatio-temporal pattern of FRET signals after ANP application to a single T-tubule shows a highly locally confined cGMP response. Scale bar, 10 µm. Quantification of experiments shown in d and h is in Fig. 6b

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