Fig. 3 | Nature Communications

Fig. 3

From: LINC complex-Lis1 interplay controls MT1-MMP matrix digest-on-demand response for confined tumor cell migration

Fig. 3

Nucleus-centrosome linkage through LINC complex is involved in MT1-MMP endosome polarization and collagenolysis-based invasion. a MDA-MB-231 cells in 3D collagen I polymerized at 37 °C treated or not with GM MMP inhibitor and stained for α-tubulin (green), centrosomal pericentrin (red) and nucleus (blue). b Galleries from representative time-lapse sequences of MDA-MB-231 cells expressing H2BmCherry (magenta) and GFPCentrin-1 (yellow) invading through type I collagen gel as in a. c Morphological analysis of DAPI-stained nuclei as in Fig. 1d. Data are mean % ± SEM from three independent experiments. (n), number of cells analyzed. P-values of Kruskal–Wallis test as compared to non-treated GFP-expressing cells. d Representative frames of a movie of MDA-MB-231 cells expressing MT1-MMPmCh (red) and H2BGFP together with GFPDN-KASH (green) invading through the small pore size collagen gel polymerized at 37 °C (see time-lapse sequence in Supplementary Movie 5). e Rose plots of endosome angular distribution from three independent experiments as in Fig. 2b. P-value for circular uniformity Rao’s Spacing test is provided. f Pericellular collagenolysis by MDA-MB-231 cells expressing GFPDN-KASH in 37 °C polymerized gel normalized to mean intensity of GFPKASHext-expressing cells ± SEM (see Supplementary Fig. 4e for representative images); n, number of cells analyzed from three independent experiments; Mann–Whitney test. g 3D invasion of GFPDN-KASH-expressing cells in the small pore size gel normalized to invasion of GFPKASHext-expressing cells ± SEM from three independent experiments as in Fig. 1l. n number of cells analyzed from three independent experiments (see Supplementary Fig. 4f for representative images); Mann–Whitney test. **P < 0.01; ****P < 0.0001; ns non significant. Scale bars=10 µm

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