Fig. 2 | Nature Communications

Fig. 2

From: LINC complex-Lis1 interplay controls MT1-MMP matrix digest-on-demand response for confined tumor cell migration

Fig. 2

Polarization of MT1-MMP endosomes is part of the adaptive MT1-MMP-dependent collagenolysis response during confined invasion. a MDA-MB-231 cells expressing MT1-MMPmCh (red) and H2BGFP (green) were embedded in the 3D small pore size collagen gel and analyzed by time-lapse confocal spinning-disk microscopy (see Supplementary Movie 1). The gallery shows representative non-consecutive frames from a representative movie obtained from three independent experiments (time in h:min). Arrows show the direction of migration. b, c Rose plots showing the percentage of MT1-MMP endosomes in 15° segments relative to the direction of nucleus movement (0°) scored from time-lapse sequences of MDA-MB-231 (b) or HT-1080 (c) cells. c number of cells, e number of endosomes analyzed from three independent experiments. P-values for circular uniformity Rao’s Spacing test are provided. d Representative frames from a time-lapse sequence of MDA-MB-231 cells invading in the large pore size collagen gel (see Supplementary Movie 2). e, f Angular distribution of MT1-MMP endosomes relative to the direction of nuclear movement as in b, c. g Gallery from a representative time-lapse sequence of MDA-MB-231 cells expressing MT1-MMPmCh (red) and GFPLMNA (green) embedded in the large pore size gel (see Supplementary Movie 3). h Rose plot of MT1-MMP endosome angular distribution from three independent experiments. i Representative frames of a movie of MDA-MB-231 cells treated with siRNA against LMNA invading through 37 °C polymerized gel (see time-lapse in Supplementary Movie 4). j Rose plot of MT1-MMP endosome angular distribution from three independent experiments. Scale bars=10 μm

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