Fig. 6 | Nature Communications

Fig. 6

From: A novel atypical sperm centriole is functional during human fertilization

Fig. 6

The remodeled DC is functional in bovine zygotes. a, b The axoneme with a CEP152-labeled DC was observed near the male pronucleus (a) and at the spindle poles (b). a Adjacent pronuclei (red), and the axoneme (arrows) at low magnification on the left. A pair of CEP152-labeled centrioles (arrowhead, right side high magnification) are found at the axoneme base. The bifurcated axoneme (arrows) is observable using DIC (lower right high magnification). b A spindle (red) with an attached axoneme (red, Ax, arrows) and a pair of CEP152-labeled centrioles (green, arrowheads, pole 2) at one pole and CEP152-labeled centrioles at the other pole (green, arrowhead, pole 1). Centrioles are within an aster (red). Scale bar 10 μm (left), and 1 μm (right). cf Zygote with closely apposed pronuclei with centrioles retained at axoneme base has separated centrioles co-labeled with CEP152 (green) and SAS-6 (red) within a single aster (blue) (f). The upper inset in c shows the centrioles relative to the pronuclei (blue). The lower inset in c shows a DIC image of the axoneme (arrows) overlaid with CEP152-labeled centrioles (box, green). The CEP152 (green, arrowheads, d) and SAS-6-labeled centrioles are within an aster (blue, arrowheads, e). The composite image (f) shows the centrioles (arrowheads) at the bifurcated axoneme base (arrows). Scale bar 10 μm (left), and 1 μm (right). gm A zygote with two separated centriole pairs (box “1” and “2” in g that each reside in an aster; blue). Upper inset in g, shows DNA (blue), and the labeled centrioles (boxes) relative to the pronucleus (blue). Lower inset in g shows a DIC image of the bifurcated axoneme (arrows) and CEP152-labeled centriole pair 1 (box). hj Enlarged images of centriole pair 1 showing details of CEP152 (green, arrowheads, h) and SAS-6 (red, arrowheads, j) within the aster (blue). Image j shows centriole pair 1 (arrowheads) at the bifurcated axoneme base (arrows). km Enlarged images of centriole pair 2 showing CEP152 (green, arrowhead, k) and SAS-6 (red, arrowheads, l) within the aster (blue). Image (m) shows centriole pair 2 (arrowheads). Scale bar 10 μm (left), and 1 μm (right)

Back to article page