Fig. 5 | Nature Communications

Fig. 5

From: A novel atypical sperm centriole is functional during human fertilization

Fig. 5

The remodeled DC is functional in vitro. ac The human remodeled DC recruits γ-tubulin from Xenopus egg extract. Demembranated human ejaculated spermatozoa did not have γ-tubulin, but after exposure to the extract, γ-tubulin was present near the DC (a). Demembranated spermatozoa with a DC inactivated by methanol pretreatment did not recruit γ-tubulin (b). Quantification (N ≥ 3) of the percent of γ-tubulin-positive sperm pretreated with either buffer or methanol (MeOH) and then exposed to extract (+) or buffer (−) (c). p < 0.001 by t-test. Scale bar 1 μm. Error bars represent +1 standard deviation

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