Fig. 4 | Nature Communications

Fig. 4

From: A novel atypical sperm centriole is functional during human fertilization

Fig. 4

POC5 and CETN 1/2 are enriched and redistributed, while CEP152 is reduced. a STORM with POC5 antibodies recognized the PC and DC (left panel). Zoom in on the DC (two middle panels) identifies two major rods (marked as “1” and “2”) and one minor rod (marked as “3”). A diagram (second row, right panel) depicting the dimensions of the rods and their distances from each other (from center to center) at their tips and bases (N ≥ 7). Scale bar 100 nm. b A section of a seminiferous tubule (left panel) depicting the changes in CEP152 (pink) and CETN1/2 (green) in various stages (cells are numbered 1–9). During spermatogenesis, CEP152 and CENT1/2 localizes to 2–4 foci in spermatogonia (cells 1–3). In spermatocytes, CEP152 is maintained, often in large foci surrounding much smaller CENT1/2 lines (cell 4). In round spermatids, CEP152 is localized to the ends of elongated CENT1/2 foci and reduces into two small foci straddling the CENT1/2 line (cells 5–6). Finally, in the elongated spermatids (cells 7–9), CEP152 is localized to the tips of the CENT1/2 “V” shape, before it is finally dramatically reduced. Scale bar 10 μm in low-magnification images (left), and 1 μm in centriole high-magnification images (right). c During spermatogenesis, CEP152 is dramatically reduced (blue), CETN1/2 is slightly reduced (yellow), and POC5 is enriched (red) (N ≥ 3). p-values determined by ANOVA with Fisher’s LSD post hoc. d A section of a seminiferous tubule (left panel) depicting the changes in the distribution of POC5 (pink) and CETN1/2 (green) in various stages (cells 1–4). Both POC5 and CENT1/2 localize to dot-shaped centrioles in spermatocytes (cell 1). In round spermatids, both POC5 and CENT1/2 elongate into a DC that is longer than the PC (cell 2). Finally, in the elongated spermatids (cells 3–4) both CETN1/2 and POC5 appear as “V” shapes. Scale bar 10 μm in low-magnification images (left), and 1 μm in centriole high-magnification images (right). e Quantification of the DC shape by POC5 immunolabeling during spermatogenesis. Most spermatogonia are dot shaped; most round spermatids are elongated; and most elongated spermatids are bifurcated (N ≥ 3, p ≤ 0.00001). p by Chi-squared