Fig. 3 | Nature Communications

Fig. 3

From: A novel atypical sperm centriole is functional during human fertilization

Fig. 3

The precise location of DC proteins. a Diagrams of the six types of DC rods morphologies. b 3D-SIM showed “V”-shaped rods of centriolar proteins POC5 and CETN1/2. c Graph depicting the abundance of the six types of DC rods morphologies. The most common type for both CETN1/2 and POC1B is “V” shape (N~140). POC5 shows both a high rate of “V” shape (~25%), and a higher rate of “reduced” shape. d A diagram depicting the dimensions of the rods (height and width) and their distances from each other (from center to center of the rods) at their tips and bases (N ≥ 20). e 3D-SIM showed “V”-shaped rods of centriolar protein POC1B flanking the splayed microtubules shown with DM1A, α-tubulin (red). f Quantification of the relationship between POC1B shape and tubulin shape in 3D-SIM. Most cells that have “V”-shaped tubulin also have “V”-shaped POC1B, suggesting that their morphology correlates. p = 0.016 by Chi-squared. g Model of the DC in the ejaculated spermatozoon based on electron microscopy, confocal microscopy, and 3D-SIM. Sc striated columns, Ax axoneme, V vault. Scale bars 1 μm