Fig. 2 | Nature Communications

Fig. 2

From: A novel atypical sperm centriole is functional during human fertilization

Fig. 2

POC1B associates with splayed microtubules at the axoneme base. a The PC and the DC were labeled with POC1B using Correlative Light and Electron Microscopy. These structures were found to be associated with the splayed microtubules (arrowheads, between the axoneme and electron light vault). Scale bars 200 nm. b Four distinct antibodies against tubulins labeled the DC and the PC with greater intensity than in the axoneme. Scale bars 1 μm. c, d Two examples of serial longitudinal sections showed splayed microtubules around an electron light vault using high-pressure freezing-freeze substitution of the sperm neck (c). Serial cross-sections showed splayed microtubules (d, arrowheads). Scale bars 200 nm. eh Models with side and top views of the human spermatozoon DC (e) and centriole (f), the fly spermatid's PCL (g), and beetle spermatid's PCL (h). Ax axoneme, M mitochondria, mp tail midpiece, N nucleus, ne neck, O outer dance fibers, pp tail principal piece, S striated columns, V vault