Fig. 2 | Nature Communications

Fig. 2

From: Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection

Fig. 2

TacT2SEn and TacT3 are functional acetyltransferase toxins. a Left panel: Growth curves of S. Typhimurium 12023 ΔtacTA2 carrying pBAD33 (−TacT2), pBAD33::tacT2 STm (+TacT2STm), pBAD33::tacT2 SEn (+TacT2SEn), pCA24N::TacA2 (+TacA2) or pBAD33::tacT2 SEn and pCA24N::tacA2 (+TacT2SEn + TacA2). Right panel: Growth curves of S. Typhimurium 12023 ΔtacTA3 carrying pBAD33 (−TacT3), pBAD33::tacT3 (+TacT3), pCA24N::TacA3 (+TacA3) or pBAD33::tacT3 and pCA24N::tacA3 (+TacT3 + TacA3). All cultures were supplemented with arabinose and IPTG in fresh rich medium during the lag phase and growth was monitored by optical density. b Left panel: Growth curves of S. Typhimurium 12023 ΔtacTA2 expressing from pBAD33, the wild-type S. Enteritidis toxin (+TacT2SEn) or point mutant toxins (+TacT2Y137FSEn) or (+TacT2R88GSEn), or carrying the empty vector (−TacT2). Right panel: Growth curves of S. Typhimurium 12023 ΔtacTA3 expressing from pBAD33, the wild-type toxin (+TacT3), or point mutant toxins (+TacT3Y143F) or (+TacT3R94E), or carrying the empty vector (−TacT3). All cultures were supplemented with arabinose in fresh rich medium during the lag phase. c Left panel: Cartoon representation of the dimeric structure of TacT3Y143F. Chain A is coloured from blue at the N terminus to red at the C terminus, and Chain B is coloured light grey. Right panel: Cartoon superimposition of TacT3Y143F (coloured) and TacTY140F (grey). TacT3- and TacT-bound Ac-CoA molecules are shown as black and grey sticks, respectively. (PDB: 6G96). d Electrostatic potential of the surface of the modelled TacT2SEn (left panel) or TacT3 (right panel) dimer showing positive potential in blue and negative in red. Residues mentioned in the text are labelled. a, b Data represent the mean ± SEM (n ≥ 3) and were analysed using a Student’s t test (ns, non-significant)

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