Fig. 1 | Nature Communications

Fig. 1

From: Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection

Fig. 1

Non-typhoidal Salmonella strains induce persister formation following internalization by human macrophages. a Fold increase in persisters caused by 30 min internalization in primary human macrophages relative to levels in inocula in clinical isolates of S. Typhimurium (white and light grey bars) and S. Enteritidis (dark grey bars), measured after exposure to gentamicin. Data represent the mean ± SEM (n ≥ 5) and were analysed using one-way ANOVA. b Comparison of the repertoire of class II TA modules between S. Typhimurium and S. Enteritidis serovars. Boxes of same colour—fully conserved; white boxes—absence; different colour boxes—polymorphism. c Proportion of bacteria surviving 4 h exposure to bactericidal concentrations of cefotaxime in cultures of S. Typhimurium 12023 wild-type, ΔtacAT2, ΔtacAT2 pBAD33::tacT2 STm , ΔtacAT2 pBAD33::tacT2 SEn , ΔtacAT2 pBAD33::tacT2 SEn and pCA24N::tacA2, or ΔtacAT3, ΔtacAT3 pBAD33::tacT3, ΔtacAT3 pBAD33::tacT3 and pCA24N::tacA3. Arabinose and IPTG were added to all cultures in the fresh medium during the lag phase, then antibiotic treatment started 1 h later. Data represent the mean ± SEM (n ≥ 3) and were analysed using a Student’s t test, compared to WT (ns, non-significant; ***p < 0.005)

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