Fig. 6 | Nature Communications

Fig. 6

From: In vivo reprogramming drives Kras-induced cancer development

Fig. 6

Prolonged expression of reprogramming factors induces poorly differentiated cancers in KC-OSKM chimeric mice. a Sall4, Lin28a, and Ki67 staining in undifferentiated pancreatic cancer induced by Dox treatment for 1 week followed by Dox withdrawal for 1 week in KC-OSKM chimeric mice. Scale bar, 50 μm. b Representative macroscopic image, histology, and Ki67 immunostaining of gastric cancer induced by prolonged transient expression of reprogramming factors in KC-OSKM chimeric mice (Dox On for 2 weeks and then Dox Off for 2 weeks). Scale bars, 100 μm. c Immunotaining for Sall4, Lin28a, and Afp in poorly differentiated gastric cancers in KC-OSKM chimeric mice. Scale bars, 100 μm. d Incidence of Afp, Sall4, and Lin28a expression in poorly differentiated gastric cancers in KC-OSKM chimeric mice. e Histology and immunostaining for SALL4, LIN28A, and LIN28B in human AFP-producing gastric cancers. Scale bar, 100 μm. f Incidence of SALL4-, LIN28A-, and LIN28B-positive cases in human AFP-producing gastric cancers. g Partial activation of ESC-Core and ESC-Myc modules in human gastric cancers expressing AFP or SALL4

Back to article page