Fig. 9 | Nature Communications

Fig. 9

From: Sculpting and fusing biomimetic vesicle networks using optical tweezers

Fig. 9

Vesicle fusion to initiate biochemical reactions in a cell-mimetic compartment. a Schematic and fluorescence microscopy images of a three-vesicle network containing the different components needed for cell-free protein expression. Red = Cy5-PE lipid, yellow = Rh-PE lipid. Dotted white circle represents unlabelled vesicle. Vesicles were fused with a laser, leading to GFP synthesis (green channel). Scale bar = 5 µm for all images. b Graph showing an increase in mean fluorescence following fusion as GFP is being synthesised (error bars = 1 s.d, n = 5)

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