Fig. 2 | Nature Communications

Fig. 2

From: A cryptic cycle in haematopoietic niches promotes initiation of malaria transmission and evasion of chemotherapy

Fig. 2

P. berghei preferentially invades early reticulocytes (ER) within bone marrow (BM) and particularly spleen. a P. berghei demonstrates a preference for infection of ER compared with erythroblasts (EB) in the BM and spleen of BALB/c mice at day 7 pi. Error bars ± SEM of individual values from the different experiments shown as points. b P. berghei does not show a preference for particular EB stages (1–3 as defined in Fig. 1) within BM and spleen of BALB/c mice as judged by percentage of invaded cell, under the assumption that the rate of random invasion is constant. Error bars ± SEM as in a. c The relative selectivity index for tissue resident erythroid cells was calculated for each animal by normalising the percentage parasitemia as a fraction relative to the smallest parasitemia. Error bars ± SEM as in a. d Numbers of total EB, though not ER are reduced in BM and spleen of infected BALB/c mice. Error bars ± SEM as in a. e Pie charts show the average proportion of each erythroblast stage in uninfected spleen (left) and infected spleen (right) of BALB/c mice. f P. berghei shows no preference for infection of erythroblast stages in the BM and spleen of BALB/c mice. Error bars ± SEM as in a. g P. berghei shows a strong preference for ER vs EB compartments in spleen and BM of BALB/c mice. Infected samples n = 9; uninfected n = 9. Error bars ± SEM as in a. h 100 GFP+ ER and EB cells were FACS sorted from spleen along with 100 GFP+ erythroid cells from the peripheral blood. n = 1. These were introduced into naive animals and the subsequent infections monitored by blood smears. Data from three independent experimental replications. Significant difference between groups or samples was tested using a one-way ANOVA alongside Dunnett’s multiple comparisons test, except for figure e, which was measured using a pair t-test. Significance is indicated with asterisks, denoting as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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