Fig. 1 | Nature Communications

Fig. 1

From: A cryptic cycle in haematopoietic niches promotes initiation of malaria transmission and evasion of chemotherapy

Fig. 1

Flow analysis robustly detects P. bergheiGFP-infected, Ter119+ CD44high erythroid cells. a Representative FACS gating strategy for bone marrow (BM) and splenic resident erythroid precursors in uninfected and P. berghei-infected animals. DNA and DNA+ erythroid cells were gated against CD44 and FS-A to resolve CD44high early reticulocytes (ER) cells and erythroblasts (EB), respectively. The DNATer119+CD44low gate was defined by the CD44 expression of erythroid cells within peripheral blood. The EB were gated in a 1:2:4 ratios to resolve immature, intermediate and mature stages, labelled 1, 2 and 3, respectively. Overlaying the DNA and DNA+ populations (composite; top right hand side of quadrant) reveals an overlap in early reticulocyte and erythroblast size. b To confirm erythroblast gates contained purely nucleated cells, populations were sorted from gates 1–3, stained with May-Grunwald and examined by light microscopy. Three representative cells from each group from different sorts are shown. Scale bar: 6 μm. c Average percentage contribution of EB, ER and Ter119+CD44low cells to the total Ter119+ cell population in the BM and spleen of BALB/c mice. d Identification of GFP expressing P. berghei parasites (PBGFPcon) in populations of EB, ER and peripheral blood erythrocytes (P) gated according to the developed protocol

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