Fig. 6 | Nature Communications

Fig. 6

From: Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons

Fig. 6

BDNFSNAP-IR700 photoablation promotes local retraction of TrkB+ afferents. ac Substantial loss of TrkBCreERT2 positive afferents (red), but persistence of other fibers (green) upon BDNFSNAP-IR700-mediated photoablation. a Innervation of paw hairy skin prior to ablation, arrows show lanceolate endings. b Loss of TrkBCreERT2 afferents after ablation, arrows show PGP9.5 fibers. c Re-innervation of skin by TrkBCreERT2 afferents at 24 days post ablation. d DRG section from control TrkBCreERT2 mouse labeled for RFP (red) and NF200 (green). e DRG section from photoablated TrkBCreERT2 mouse labeled for RFP (red) and NF200 (green). f Quantification of the proportion of hair follicle innervation and DRG neurons positive for TrkB following photoablation in the paw and the quantification of PGP9.5+ free nerve endings showing the numbers of free nerves remain unaffected. Representative skin sections from control and BDNFSNAP-IR700 photoablated mice labeled with the indicated antibodies. TrkB-positive cells are indicated in red and DAPI-positive nuclei in blue. g, h Keratinocytes labeled with K14 (green). i Quantification of the number of K14+ cells. j, k Dendritic cells and dermal antigen-presenting cells labeled with MHC-II (green) and l the quantification for MHC-II+ cells. m, n Mast cells and epithelial and endothelial progenitor cells labeled with CD34 (green). o Quantification of the number of K14+ cells. Scale bars, 40 µm. Error bars indicate SEM

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