Fig. 5 | Nature Communications

Fig. 5

From: Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons

Fig. 5

Optopharmacological targeting of TrkB+ neurons with BDNFSNAP. a Labeling (inset) and quantification of dissociated DRG from TrkBCreERT2::Rosa26RFP mice with BDNFSNAP shows substantial overlap of BDNFSNAP binding to TrkB+ cells (n = 4, 450 cells). b Schematic representation of BDNFSNAP-IR700 injection and photoablation. c BDNFSNAP-IR700-mediated photoablation of the paw of SNI mice results in a dose-dependent reversal of mechanical hypersensitivity as assayed with von Frey filaments (n = 10, two-way RM ANOVA; p = 0.003 followed by a Bonferroni post hoc test) and d dynamic brush stimuli (t-test; p = 0.016). e Hypersensitivity to cotton swab is also reversed by photoablation (t-test: p = 0.042). f BDNFSNAP-IR700-mediated photoablation reverses mechanical allodynia in the streptozotocin (STZ) model of diabetic neuropathy (n = 5, two-way RM ANOVA; p = 0.007 followed by a Bonferroni post hoc test. Open circles; 5 µM BDNFSNAP-IR700 at 200 J/cm2, closed circles, 5 µM IR700 at 200 J/cm2). g BDNFSNAP-IR700-mediated photoablation reverses mechanical allodynia in the paclitaxel (PTX) model of chemotherapy-induced neuropathy (n = 5, two-way RM ANOVA; p = 0.027 followed by a Bonferroni post hoc test. Open circles; 5 µM BDNFSNAP-IR700 at 200 J/cm2, closed circles, 5 µM IR700 at 200 J/cm2). Error bars indicate SEM

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