Fig. 3 | Nature Communications

Fig. 3

From: Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons

Fig. 3

Diphtheria toxin-mediated ablation of TrkB+ sensory neurons. Immunostaining of DRG sections of TrkBCreERT2::AviliDTR mice with an antibody against the diphtheria toxin receptor (red) from a untreated mice and b after i.p. injections of diphtheria toxin. c Quantification of DRG sections indicating a ~90% decrease in TrkBDTR and TrkBmRNA cells after ablation and 36% reduction in NF200+ neurons without affecting other subpopulations. dl Behavioral responses in littermate control mice (AviliDTR, black bars) and TrkBCreERT2::AviliDTR mice (white bars) showing no differences in responses before and after ablation in the acetone drop test (t-test; p = 0.800) on d glabrous and e hairy skin, f hot plate test (t-test; p = 0.914), g radiant heat test on glabrous and hairy skin (t-test; p = 0.263), h grip test (t-test; p = 0.484), i pin-prick test (t-test; p = 0.691), j tape test (t-test; p = 0.776), and k punctate mechanical flick test applied to the hairy back skin (t-test; p = 0.196). l Ablated mice show a reduction in sensitivities to cotton swab (t-test, p = 0.002). Scale bars in a, b 50 μm, error bars indicate SEM

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