Fig. 2 | Nature Communications

Fig. 2

From: Pathogen-derived extracellular vesicles mediate virulence in the fatal human pathogen Cryptococcus gattii

Fig. 2

Capsular material is necessary but not sufficient to increase the IPR of non-outbreak cryptococci. a Differential interference contrast (DIC; left), India Ink (middle) and immunostained (MAb 18B7; right) images of wild-type R265 (top) and acapsular R265ΔCap10 (bottom), indicating the presence of the characteristic thick polysaccharide capsule in the wild-type (arrows). Note the lack of any visible capsule in the acapsular mutant. Bars: 5 and 2 μm. b IPR of a non-virulent ICB180 strain is not altered by the presence of acapsular R265ΔCap10 in the transwell assay (ICB180(+R265ΔCap10)), nor by the addition of capsular material isolated from R265 (ICB180+R265 capsule100 ng; ICB180+R265 capsule5 μg). An addition into the transwell of acapsular R265ΔCap10 strain mixed with the capsular material isolated from R265 also did not change the IPR of ICB180 (ICB180(+R265ΔCap10+R265 capsule)). Data are presented as scattered dot plots with lines representing their medians. Individual Wilcoxon matched-pairs signed rank test presented as P values above each dot plot, where ns (P > 0.05), not significantly different. Data are representative of results from at least nine independent experiments with 808–1913 total number of yeasts counted for each sample

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