Fig. 2 | Nature Communications

Fig. 2

From: Modular assembly of proteins on nanoparticles

Fig. 2

The role of GST thiols in the binding to GNPs. a Equilibrium binding data of NEM-GST fitted to the binding model (dashed line) show no significant difference with those of GST (dotted line, obtained from Fig. 1b; 95% confidence intervals for KD and ΔdMAX estimates are represented in Supplementary Figure 3). b UV–vis spectra of naked GNPs (CTRL) compared to GNPs coated with GST or NEM-GST. Vertical dashed lines indicate the two wavelengths, 525 and 600 nm, that have been used for calculation of the Au–S index. c UV–vis spectra of the same colloids in b but after treatment with 2-ME. d Au–S stability index measured at different times shows an increased stability of GST/GNPs over time, whereas NEM-GST failed to protect from aggregation at any time point. GST chemisorption is represented as the fit of the Au–S index data to a sigmoid (dashed line). This is compared to the calculated physisorption trend (dotted line), obtained from Eq. 1 for the same GST concentration used in the chemisorption experiment. e SERS spectra of GST and NEM-GST adsorbed on silver colloid. The black arrow indicates the peak at 233 cm−1 due to Ag–S mediated chemisorption. All data points with error bars in the figure represent average and standard deviation of three measurements

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