Fig. 7 | Nature Communications

Fig. 7

From: Contraction of basal filopodia controls periodic feather branching via Notch and FGF signaling

Fig. 7

The filopodia sense and transport FGF10 and increase the surface area for feather branching. a SU5402 induced ectopic branch (eBr) in the rachis and inhibited filopodia. b Locally implanted FGF10 beads inhibited branching and induced filopodia in basal keratinocytes. c Filopodia expressed FGFR1, and transported FGF10. Notice the FGF10 puncta (red) were lined up along the filopodia (green). d A TEM image showing the transported vesicles inside the filopodia (marked by arrowheads). Dashed green line indicated the basal lamina. e The circumference (λ) and distance (d) of the epithelial—mesenchymal border were measured, and their ratio was calculated. Ten follicles were analyzed and representative images are shown. **, p< 0.01 by t-test. f Schematics showing FGF signaling cooperates with periodic Notch activation to regulate the filopodia and E-Cadherin/β-Catenin-mediated cell adhesion, which then control the periodic feather branching. Βar = 50 μm in a, b, e; 10 μm in c; 2 μm in the TEM images