Fig. 6 | Nature Communications

Fig. 6

From: Contraction of basal filopodia controls periodic feather branching via Notch and FGF signaling

Fig. 6

A proximal-distal FGF signaling gradient regulates feather branching. a Semi-quantitative RT-PCR analysis revealed graded expression of FGF10 and FGF2 in the feather follicle. The dermal papillae (dp) and the pulp represent the proximal and distal compartment of the follicle, respectively. b In situ hybridization and c immunofluorescence showing the graded expression of FGF2 and FGF10 in the feather follicle. d Whole mount view and HE sections of the feather rachis after manipulating the FGF signaling via RNAi-FGFR1 lentivirus local injection, or SU5402 (100 μM) chemical inhibitor treatment in vivo. Ectopic branches were induced by both treatments. Representative images from five repeated experiments are shown. Ra rachis, eBr ectopic branch. Bar = 100 μm