Fig. 5 | Nature Communications

Fig. 5

From: Contraction of basal filopodia controls periodic feather branching via Notch and FGF signaling

Fig. 5

Notch signaling regulates feather branching and the contraction of filopodia. a–d The expression patterns of Notch1, Notch2, Ser1, Ser2 in feather branching. e–h Phenotypes of Notch1 and Notch2 manipulation in the feather follicle. The numbers indicate the occasions of feathers with the phenotype. i, j The expression patterns of downstream target genes L-fringe and Hey1 in the feather follicle. k, l Expression of a 6XCSL-GFP reporter was specifically in the marginal plate of branching feather epithelium, whereas a control viral vector showed widespread virus expression in the follicle. m A secretory form of Ser2 (sSer2) induced ectopic rachis formation. n Ectopic activation of Notch signaling via local injection of RNAi-Notch1 lentivirus led to filopodia contraction and E-Cadherin down-regulation in the marginal plate cells (arrow head). Representative images from three repeated experiments are shown. mp, marginal plate; bp, barb plate. Bar = 100 μm in a–d and i–l (shown in i); 2 mm in e–h and m (shown in m); 20 μm in n