Fig. 3 | Nature Communications

Fig. 3

From: Contraction of basal filopodia controls periodic feather branching via Notch and FGF signaling

Fig. 3

Filopodia regulate feather branching. a, b Schematics showing two different methods for manipulating gene expression in the feather follicle. Lentivirus were either directly injected into the developing follicle (a), or into the follicle cavity after plucking the feather (b). c, d The dominant negative (DN) forms of RhoA and Cdc42, but not Rac1 nor their constitutively active (CA) forms regulated filopodia in vivo as shown by β-Catenin staining (c, local injection in the rachis region) and the final feather form (d, transgenic feathers). e RNAi knockdown of RhoA or Cdc42, but not Rac1 disrupted feather axis formation. The numbers indicate the occasions of feathers with the phenotype. Bar = 20 μm in c, 2 mm in d, e