Fig. 2 | Nature Communications

Fig. 2

From: Contraction of basal filopodia controls periodic feather branching via Notch and FGF signaling

Fig. 2

Filopodia in basal keratinocytes of the feather epithelium. a A schematic diagram showing the regions examined. The proximal feather epithelium (collar, Cl; regions 1, 3) is not branched. Above the ramogenic zone (Rz), the epithelium branches along the circumference of the follicle, with the exception of the rachis in the anterior (Ra, region 2). Filopodia were found in regions 1, 2, 3 but not 4. b HE staining; c, d TEM analysis; e immunofluorescence showing filopodia in the basal keratinocytes of the pre-branch feather epithelium. Basal lamina are shown in higher magnifications in c, with the lamina lucida indicated by dots, and lamina densa indicated by arrow heads. Tight junction (TJ) and adherens junctions (AJ) are indicated in the basal keratinocytes (d and inset). Dashed green line indicates the basal lamina. Representative images from five repeated experiments are shown. Bar = 20 μm in b, e, 2 μm in c, 0.3 μm in d

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