Pulmonary arterial hypertension (PAH) is a rare disorder with a poor prognosis. Deleterious variation within components of the transforming growth factor-β pathway, particularly the bone morphogenetic protein type 2 receptor (BMPR2), underlies most heritable forms of PAH. To identify the missing heritability we perform whole-genome sequencing in 1038 PAH index cases and 6385 PAH-negative control subjects. Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in PAH. We demonstrate familial segregation of mutations in SOX17 and AQP1 with PAH. Mutations in GDF2, encoding a BMPR2 ligand, lead to reduced secretion from transfected cells. In addition, we identify pathogenic mutations in the majority of previously reported PAH genes, and provide evidence for further putative genes. Taken together these findings contribute new insights into the molecular basis of PAH and indicate unexplored pathways for therapeutic intervention.
Idiopathic and heritable pulmonary arterial hypertension (PAH) are rare disorders characterised by occlusion of arterioles in the lung1, leading to marked increases in pulmonary vascular resistance2. Life expectancy from diagnosis averages 3–5 years3, with death ensuing from failure of the right ventricle.
Mutations in the gene encoding the bone morphogenetic protein type 2 receptor (BMPR2), a receptor for the transforming growth factor-beta (TGF-β) superfamily4,5 account for over 80% of families with PAH, and approximately 20% of sporadic cases6. Mutations have been identified in genes encoding other components of the TGF-β/bone morphogenetic protein (BMP) signalling pathways, including ACVRL17 and ENG8. On endothelial cells, BMPR2 and ACVRL1 form a signalling complex, utilising ENG as a co-receptor. Case reports of rare sequence variation in the BMP signalling intermediaries, SMAD1, SMAD4 and SMAD99,10, provide compelling evidence for a central role of dysregulated BMP signalling in PAH pathogenesis.
Analysis of coding variation in BMPR2-negative kindreds revealed heterozygous mutations in genes not directly impacting on the TGF-β/BMP pathway, including CAV111, and the potassium channel, KCNK312. Deletions and loss of function mutations in TBX4, an essential regulator of embryonic development, were identified in childhood-onset PAH13. A clinically and pathologically distinct form of PAH, known as pulmonary veno-occlusive disease or pulmonary capillary haemangiomatosis (PVOD/PCH), was shown recently to be caused by biallelic recessive mutations in EIF2AK414,15, a kinase in the integrated stress response.
The purpose of the present study was to identify additional rare sequence variation contributing to the genetic architecture of PAH, and to assess the relative contribution of rare variants in genes implicated in prior studies. A major finding is that rare likely causal heterozygous variants in several previously unidentified genes (ATP13A3, AQP1 and SOX17) were significantly overrepresented in the PAH cohort, and we provide independent validation for GDF2 as a causal gene.
Description of the PAH cohort
In total, 1048 PAH cases (1038 index cases and 10 related affected individuals) were recruited for WGS. Of these, 908 (86.7%) were diagnosed with idiopathic PAH, 58 (5.5%) gave a family history of PAH and 60 (5.7%) gave a history of drug exposure associated with PAH16. Twenty two cases (2.1%) held a clinical diagnosis of PVOD/PCH (Fig. 1a). Demographic and clinical characteristics of the PAH cohort are provided in Supplementary Table 1. An additional UK family was recruited separately for novel gene identification studies. Briefly, the proband was diagnosed at 12 years with a persistent ductus arteriosus and elevated pulmonary arterial pressure. Explant lung histology following heart-lung transplantation revealed the presence of plexiform lesions. Two of the proband’s offspring were also diagnosed with childhood-onset PAH, one of which had an atrial septal defect. The proband’s parents, siblings and a third child showed no evidence of cardiovascular disease.
Pathogenic variants in previously reported PAH disease genes
Our filtering strategy detected rare deleterious variation in previously reported PAH genes in 19.9% of the PAH cohort. For BMPR2, rare heterozygous mutations were identified in 160 of 1048 cases (15.3%). The frequency of BMPR2 mutations in familial PAH was 75.9%, in sporadic cases 12.2%, and 8.3% in anorexigen-exposed PAH cases. Forty-eight percent of BMPR2 mutations were reported previously17, and the remainder were newly identified in this study. Fourteen percent of BMPR2 mutations resulted in the deletion of larger protein-coding regions ranging from 5 kb to 3.8 Mb in size. Supplementary Data 1 provides the breakdown of BMPR2 SNVs and indels, and the larger deletions are shown in Fig. 2a–c with a detailed summary in Supplementary Table 2.
Of the other genes previously reported in PAH we identified deleterious heterozygous rare variants in ACVRL1 (9 cases, 0.9%), ENG (6 cases, 0.6%), SMAD9 (4 cases, 0.4%), KCNK3 (4 cases, 0.4%), and TBX4 (14 cases, 1.3%). We identified one case with highly deleterious variants in both BMPR2 (p.Cys123Arg) and SMAD9 (p.Arg294Ter). Details of consequence types, deleteriousness and conservation scores, and minor allele frequencies are provided in Supplementary Data 2. Fourteen cases (1.3%) with biallelic EIF2AK4 mutations were found18. No pathogenic coding variants in CAV1, SMAD1 or SMAD4 were identified. Taken together, rare causal variation in non-BMPR2 disease genes (TBX4, ENG, ACVRL1, SMAD9, KCNK3 and EIF2AK4) accounted for 4.7% of the entire PAH cohort. The clinical characteristics of cases with variants in these previously reported genes are shown in Supplementary Table 3.
In a case-control comparison of the frequencies of deleterious variants confined to the previously reported PAH genes, we observed significant overrepresentation of rare variants in BMPR2, TBX4, ACVRL1 and biallelic variants in EIF2AK4 only (P < 0.05; Supplementary Table 4).
Identification of novel PAH disease genes
The strategy to identify novel causative genes in PAH employed a series of case-control analyses (Fig. 1b). To identify signals that might be masked by variants in previously reported PAH genes, we excluded subjects with rare variants and deletions in BMPR2, EIF2AK4, ENG, ACVRL1, TBX4, SMAD9 and KCNK3. A genome-wide comparison of protein-truncating variants (PTVs), representative of high impact variants, identified a higher frequency of PTVs in ATP13A3 (six cases) (Padj = 0.0346). Moreover, we identified additional PTVs in several putative PAH genes, including EVI5 (5 cases, 1 control) and KDR (4 cases, 0 controls; Fig. 3a), that require further validation to evaluate their contribution to PAH pathogenesis (Supplementary Table 5).
We next analysed rare missense variants overrepresented in the PAH cohort, again excluding subjects with variants in the previously reported PAH genes. This revealed significant overrepresentation of rare variants in GDF2 after correction for multiple testing (Padj = 0.0023), followed by AQP1 (Fig. 3b and Supplementary Table 6). Next, in a combined analysis of rare missense variants and PTV, only GDF2 remained significant (P = 0.001). Rare variants in additional putative genes occurred at higher frequency in cases compared to controls, including AQP1, ALPPL2, ATP13A3, OR8U1, IFT74, FLNA, SOX17, ATP13A5, C3orf20 and PIWIL1 (uncorrected P value < 0.0005), but were not significant after correction for multiple testing (Fig. 3c and Supplementary Table 7).
In order to increase power to detect rare associations, we deployed SKAT-O on filtered rare PTVs and missense variants. Excluding previously reported genes, this analysis revealed an association with rare variants in AQP1 (Padj = 4.28 × 10−6) and SOX17 (Padj = 6.7 × 10−5; Fig. 3d). AQP1 and SOX17 were both also nominally significant in the combined burden tests, described above. Association was also found with rare variants in MFRP (Padj = 1.3 × 10−5). However, we consider MFRP a false-positive finding for reasons given in the Discussion. Supplementary Table 8 shows the top 50 most significant genes identified by SKAT-O, providing further candidates to be evaluated in future studies. Details of rare variants in novel PAH genes (GDF2, ATP13A3, AQP1, SOX17) identified in cases are provided in Supplementary Data 3.
Notably, a genome-wide assessment of larger structural variation did not identify any additional large deletions after exclusion of subjects harbouring deletions in BMPR2 (Fig. 2d, e).
The proportion of PAH cases with mutations in the new genes was 3.5%. The clinical characteristics of PAH cases with mutations in these genes are provided in Supplementary Table 3b. Of note, cases with mutations in SOX17 and AQP1 were significantly younger at diagnosis (32.8 ± 16.2 years (P = 0.002) and 36.9 ± 14.3 years (P = 0.013), respectively) compared to cases with no mutations in the previously established genes (51.7 ± 16.6 years).
Non-coding variation around PAH disease genes
An initial analysis for enrichment of variants in the non-coding sequence surrounding previously reported and newly identified PAH disease genes, including upstream gene regions, 5’ UTRs, intronic sequence, 3’ UTRs and downstream gene regions, did not detect an significant overrepresentation in the PAH cohort. Details of the non-coding variants that passed the filtering strategy are provided in Supplementary Data 4.
Independent validation and familial segregation analysis
To provide further validation of the potentially causal role of mutations in the new genes identified, we examined whole-exome data from an independent UK family with three affected individuals across two generations. Microsatellite genotyping across chromosome 2q33 had previously demonstrated non-sharing of haplotypes in affected individuals, consistent with exclusion of linkage to the BMPR2 locus. No pathogenic variants were identified in the protein-coding regions of the BMPR2 gene or other TGF-β pathway genes. Analysis of exome sequence data from individual II-1 identified a novel heterozygous c.411 C > G (p.Y137*) PTV in the SOX17 gene. Segregation analysis in the extended family demonstrated that the mutation had arisen de novo in the affected father (II-1) and was transmitted to the affected offspring (III-1). All unaffected family members were confirmed as wild-type (Fig. 4a).
Three HPAH subjects harbouring rare variants in AQP1, identified in the NIHR BR-RD WGS study, were also selected for familial co-segregation analysis (Fig. 4b–d). No pathogenic variants in any of the previously reported genes were identified in these families. The first pedigree comprised three affected individuals across two generations. Sanger sequencing confirmed the presence of the heterozygous AQP1 c.583 C > T (p.R195W) missense variant in the proband (E011942), the affected father (E011942.f) and the healthy younger paternal uncle (E011942.u1). An additional unaffected uncle did not carry the AQP1 variant. These results indicate likely incomplete penetrance in the unaffected carrier, as observed in BMPR2 families19. No additional clinical information was available for the deceased grandparents (Fig. 4b). The remaining two families comprised affected parent-offspring individuals. By Sanger sequencing we independently confirmed a heterozygous AQP1 c.527 T > A (p.Val176Glu) missense variant in proband (E012415) and his affected father (Fig. 4c), as well as a heterozygous AQP1 c.583 C > T (p.R195W) missense variant in proband (E010634) and her affected father (Fig. 4d). These results highlight recurrent AQP1 variation across unrelated families and demonstrate co-segregation with the phenotype.
Predicted functional impact of variants in novel PAH genes
To evaluate the potential functional impact of rare variants identified in the likely causative new genes we performed structural analysis of GDF2, ATP13A3, AQP1, and SOX17. In addition we undertook a functional analysis of the GDF2 variants identified.
Heterozygous mutations in GDF2 exclusive to PAH cases comprised 1 frameshift variant and 7 missense variants. GDF2 encodes growth and differentiation factor 2, also known as bone morphogenetic protein 9 (BMP9), the major circulating ligand for the endothelial BMPR2/ACVRL1 receptor complex20. Amino acid substitutions were assessed against the published crystal structure21 of the prodomain bound form of GDF2 (Fig. 5). Variants clustered at the interface between the prodomain and growth factor domain. Since the prodomain is important for the processing of GDF2, it is likely that amino acid substitutions reduce the stability of the prodomain-growth factor interface. In keeping with these predictions, HEK293T cells transfected with GDF2 variants exclusive to PAH cases, demonstrated reduced secretion of mature GDF2 into the cell supernatants (Fig. 5d), compared with wild-type GDF2.
We identified three heterozygous frameshift variants, two stop gained, two splice region variants in ATP13A3, which are predicted to lead to loss of ATPase catalytic activity (Fig. 6a). In addition, we identified 4 heterozygous likely pathogenic missense variants in PAH cases, two near the conserved ATPase catalytic site and predicted to destabilise the conformation of the catalytic domain (Fig. 6b–d). The distribution of variants (Fig. 6a) suggests that these mutations impact critically on the function of the protein.
The majority of rare variants identified in AQP1, which encodes aquaporin-1, are situated within the critical water channel (Fig. 7). In particular the p.Arg195Trp variant, identified in five PAH cases, locates at the hydrophilic face of the pore. This arginine at position 195 helps define the constriction region of the AQP1 pore structure and is conserved across the water-specific aquaporins22. Rare variants in SOX17, included four nonsense variants (including the PTV identified in the additional UK family) predicted to lead to loss of the beta-catenin binding region, and six missense variants predicted to disrupt interactions with Oct4 and beta-catenin23,24 (Fig. 8).
GDF2 is known to be secreted from the liver, but the cellular localisation of proteins encoded by the other novel genes is less well characterised. Thus we employed immunohistochemistry to examine localisation in the normal and hypertensive human pulmonary vasculature. Figure 9 shows that AQP1, ATP13A3 and SOX17 are predominantly localised to the pulmonary endothelium in normal human lung and to endothelial cells within plexiform lesions of patients with idiopathic PAH. In addition, we determined the relative mRNA expression levels of AQP1, ATP13A3 and SOX17 in primary cultures of pulmonary artery smooth muscle cells (PASMCs), pulmonary artery endothelial cells (PAECs) and blood outgrowth endothelial cells (BOECs)25. AQP1 was expressed in PASMCs and endothelial cells, with a trend towards higher levels in PASMCs (Fig. 10a). ATP13A3 was highly expressed in both cell types (Fig. 10b), whereas SOX17 was almost exclusively expressed in endothelial cells (Fig. 10c). Although AQP1 and SOX17 are known to play roles in endothelial function, the function of ATP13A3 in vascular cells is entirely unknown. Thus, we determined the impact of ATP13A3 knockdown on proliferation and apoptosis of BOECs. Loss of ATP13A3 led to marked inhibition of serum-stimulated proliferation of BOECs, and increased apoptosis in serum-deprived conditions (Fig. 10d–f).
We report a comprehensive analysis of rare genetic variation in a large cohort of index cases with idiopathic and heritable forms of PAH. Whilst we utilised WGS, the main goal was the identification of rare causal variation underlying PAH in the protein-coding sequence. The approach involved a rigorous case-control comparison using a tiered search for variants. First, we searched for high impact PTVs overrepresented in cases, having excluded previously established PAH genes. This revealed PTVs in ATP13A3, a poorly characterised P-type ATPase of the P5 subfamily26. There is little information regarding the function of the ATPase, ATP13A3, which appears widely expressed in mouse tissues26. Although, the precise substrate specificity is unknown, ATP13A3 plays a role in polyamine transport27. Based on available RNA sequencing data, ATP13A3 is highly expressed in human pulmonary vascular cells and cardiac tissue (https://www.encodeproject.org). We confirmed that ATP13A3 mRNA is expressed in primary cultured pulmonary artery smooth muscle cells and endothelial cells, and provide preliminary data that loss of ATP13A3 inhibits proliferation and increases apoptosis of endothelial cells. These findings are consistent with the widely accepted paradigm that endothelial apoptosis is a major trigger for the initiation of PAH28,29. It will be of considerable interest to determine the role of ATP13A3 in vascular cells and whether it is functionally associated with BMP signalling, or represents a distinct therapeutic target in PAH.
Analysis of missense variation, and a combined analysis of all predicted deleterious variation, revealed that mutation at the GDF2 gene is also significant determinant of predisposition to PAH. Of the new genes identified, GDF2 provides further evidence for the central role of the BMP signalling pathway in PAH. GDF2 encodes the major circulating ligand for the endothelial BMPR2/ACVRL1 receptor complex20. Taken together, the genetic findings suggest that a deficiency in GDF2/BMPR2/ACVRL1 signalling in pulmonary artery endothelial cells is critical in PAH pathobiology. The majority of GDF2 variants detected in our adult-onset PAH cohort were heterozygous missense variants, in contrast to a previous case report of childhood-onset PAH due to a homozygous nonsense mutation30. The finding of causal GDF2 variants in PAH cases, associated with reduced production of GDF2 from cells, provides further support for investigating replacement of this factor as a therapeutic strategy in PAH31.
To maximise the assessment of rare variation in a case-control study design, we deployed the SKAT-O test. This approach revealed a significant association of rare variation in the aquaporin gene, AQP1, and the transcription factor encoded by SOX17. Of note, both AQP1 and SOX17 were within the top 8 ranked genes in our combined PTV and missense burden test analysis (Supplementary Table 7), providing further confidence in their causative contribution to PAH.
Aquaporin-1 belongs to a family of membrane channel proteins that facilitate water transport in response to osmotic gradients22, and AQP1 is known to promote endothelial cell migration and angiogenesis32. Thus, approaches that maintain or restore pulmonary endothelial function could offer new therapeutic directions in PAH. Conversely, AQP1 inhibition in pulmonary artery smooth muscle cells ameliorated hypoxia-induced pulmonary hypertension in mice33, suggesting that further studies are required to determine the key cell type impacted by AQP1 mutations in human PAH, and the functional impact of these AQP1 variants on water transport. The demonstration of familial segregation of AQP1 variants with PAH provides further support for the potentially causal role of these mutations in disease. However, we also identified an unaffected AQP1 variant carrier consistent with reduced penetrance, which is well described for other PAH genes, including BMPR2.
Although functional studies are required to confirm the mechanisms by which mutations in SOX17 cause PAH, this finding provides additional support for the vascular endothelium as the major initiating cell type in this disorder. SOX17 encodes the SRY-box containing transcription factor 17, which plays a fundamental role in angiogenesis34 and arteriovenous differentiation35. Moreover, conditional deletion of SOX17 in mesenchymal progenitors leads to impaired formation of lung microvessels36. The demonstration of familial segregation of the SOX17 p.Y137* PTV with early onset PAH provides additional evidence for a causal role for these variants in PAH. The co-existence of a patent ductus arteriosus in the index case and an atrial septal defect (ASD) in one of the affected offspring is of interest and suggests an association with congenital heart disease. Small ASDs are not uncommon in idiopathic PAH, and a more detailed clinical phenotyping of SOX17 mutation carriers will be required to determine whether the presence of ASDs and other congenital heart abnormalities are more common in carriers of these mutations.
Whilst the SKAT-O analysis also provided support for the MFRP gene, recessive biallelic mutations in MFRP cause retinal degeneration and posterior microphthalmos37. The expression of MFRP transcripts is largely confined to the central nervous system38 and the majority of variants were present in the Genome Aggregation Database (GnomAD, http://gnomad.broadinstitute.org). On the basis of these considerations, variants in MFRP are unlikely to contribute to PAH aetiology.
This analysis provides new insights on the frequency and validity of previously reported genes in PAH. We confirmed that mutations in BMPR2 are the most common genetic cause and validated rare causal variants in ACVRL1, ENG, SMAD9, TBX4, KCNK3 and EIF2AK4. Although our findings question the validity of CAV1, SMAD1 and SMAD4 as causal genes, previous reports might represent private mutations occurring in very rare families. The use of WGS in this study allowed closer interrogation of larger deletions around the BMPR2 locus than has been possible previously. Nevertheless, additional analyses are required to determine the full impact of structural variation (inversions, duplications, smaller deletions) at this and other loci.
The non-PAH cohort used in the case-control comparisons for this study comprised individuals, or relatives of individuals, with other rare diseases recruited to the NIHR BR-RD in the UK (Methods section). In general, for very rare causal variants, the comparison between PAH cases and non-PAH rare disease controls should not reduce our ability to detect overrepresentation of rare variants in a particular gene in the PAH cohort, if mutations in that gene are specific to PAH. However, if rare variants in a gene were responsible for more than one phenotype, it is possible that this would reduce the power to detect overrepresentation in the PAH cohort. For example, if mutations occurred in different functional domains of the expressed protein, this might lead to PAH if mutations affected one domain, but other phenotypes if they affected another domain. Overcoming this potential limitation will require additional analysis of the functional impact of variants and their distribution within a gene, and more detailed information on the phenotypes of subjects in the non-PAH group.
Taken together, this study identifies rare sequence variation in new genes underlying heritable forms of PAH, and provides a unique resource for future large-scale discovery efforts in this disorder. Mutations in previously established genes accounted for 19.9% of PAH cases. Including new genes identified in this study (GDF2, ATP13A3, AQP1, and SOX17), the total proportion of cases explained by mutations increased to 23.5%. It is likely that independent confirmation of the expanded list of putative genes identified in this study will increase further the proportion of cases explained by mutations, but this will require larger international collaborations. The results suggest that the genetic architecture of PAH, beyond mutations in BMPR2, is characterised by substantial genetic heterogeneity and consists of rare heterozygous coding region mutations shared by small numbers of cases. The contribution of rare variation within non-coding regulatory regions to PAH aetiology remains to be determined. This will require functional annotation of regulatory and other non-coding regions specific for relevant cell types, further case-control analyses of these regions and ultimately functional studies of gene regulation to assess the pathogenicity of non-coding variants. Our findings to date provide support for a central role of the pulmonary vascular endothelium in disease pathogenesis, and suggest new mechanisms that could be exploited therapeutically in this life-limiting disease.
Ethics and patient selection
Cases were recruited from the UK National Pulmonary Hypertension Centres, Universite Sud Paris (France), the VU University Medical Center Amsterdam (The Netherlands), the Universities of Gießen and Marburg (Germany), San Matteo Hospital, Pavia (Italy), and Medical University of Graz (Austria). All cases had a clinical diagnosis of idiopathic PAH, heritable PAH, drug-associated and toxin-associated PAH, or PVOD/PCH established by their expert centre. The non-PAH cohort for the case-control comparison comprised 6385 unrelated subjects recruited to the NIHR BR-RD study. All PAH and non-PAH patients provided written informed consent (UK Research Ethics Committee: 13/EE/0325), or local forms consenting to genetic testing in deceased patients and non-UK cases. An additional UK family diagnosed with HPAH was ascertained as described previously39. Blood and saliva samples were collected under written informed consent of the participants or their parents for use in gene identification studies (UK Research Ethics Committee: 08/H0802/32).
Composition of non-PAH control cohort
The non-PAH control cohort consisted of subjects with bleeding, thrombotic and platelet disorders (15.5%), cerebral small vessel disease (2.1%), Ehlers-Danlos syndrome (0.3%), subjects recruited to Genomics England Ltd (19.8%), hypertrophic cardiomyopathy (3.6%), intrahepatic cholestasis of pregnancy (4.1%), Leber hereditary optic neuropathy (0.9%), multiple primary tumours (7.8%), neuropathic pain disorder (2.6%), primary immune disorders (15.3%), primary membranoproliferative glomerulonephritis (2.3%), retinal dystrophies/paediatric neurology and metabolic disease (19.8%), stem cell and myeloid disorders (2.1%), steroid resistant nephrotic syndrome (3.6%), and others (0.3%), or their first degree relatives.
DNA extracted from venous blood underwent whole-genome sequencing using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) and Illumina HiSeq 2000 or HiSeq X sequencer, generating 100–150 bp reads with a minimum coverage of 15× for ~95% of the genome (mean coverage of 35×). Whole-exome sequencing was conducted for individual II-1 (Fig. 4a) using genomic DNA extracted from peripheral blood. Paired-end sequence reads were generated on an Illumina HiSeq 2000.
Generation of analysis-ready data sets
Sequencing reads were pre-processed by Illumina with Isaac Aligner and Variant Caller (v2, Illumina Inc.) using human genome assembly GRCh37 as reference. Variants were normalised, merged into multi-sample VCF files by chromosome using the gVCF aggregation tool agg (https://github.com/Illumina/agg) and annotated with Ensembl’s Variant Effect Predictor (VEP). Following read alignment to the reference genome (GRCh37), variant calling and annotation of whole-exome data for individual II:1 were performed using GATK UnifiedGenotyper40 and ANNOVAR41, respectively. Annotations included minor allele frequencies from other control data sets (i.e. ExAC42, 1000 Genomes Project43 and UK10K44) as well as deleteriousness and conservation scores (i.e., CADD45, SIFT46, PolyPhen-247 and Gerp48) enabling further filtering and assessment of the likely pathogenicity of variants. To take forward only high quality calls, the pass frequency (proportion of samples containing alternate alleles that passed the original variant filtering) and call rate (proportion of samples with reference or alternate genotypes) were combined into the overall pass rate (OPR: pass frequency × call rate) and variants with an OPR of 80% or higher were retained.
Estimation of ethnicity and relatedness
We estimated the population structure and relatedness based on a representative set of SNPs using the R package GENESIS to perform PC Air49 and PC Relate50, respectively. The selected 35,114 autosomal SNPs were present on Illumina genotyping arrays (HumanCoreExome-12v1.1, HumanCoreExome-24v1.0, HumanOmni2.5–8v1.1), do not overlap quality control excluded regions or multiallelic sites in the 1000 Genomes (1000 G) Phase 3 data set43, do not have any missing genotypes in NIHR BR-RD, had a MAF of 0.3 or above and LD pruning was performed using PLINK51 with a window size of 50 bp, window shift of 5 bp and a variance inflation factor threshold of 2. The 2,110 samples from the 1000 G Project including the European (EUR), African (AFR), South Asian (SAS) and East Asian (EAS) populations (excluding the admixed American population) were filtered for the selected SNPs and the filtered data were used to perform a principal component analysis (PCA) using PC Air. We modelled the scores of the leading five principal components as data generated by a population specific multivariate Gaussian distribution and estimated the corresponding mean and covariance parameters. Genotypes from the NIHR BR-RD samples were projected onto the loadings for the leading five principal components from the 1000 G PCA and we computed the likelihood that each sample belonged to each subpopulation under a mixture of multivariate Gaussians models. Each sample was allocated to the population with the highest likelihood, unless the highest likelihood was similar to likelihood values for other populations, as might be expected for example under admixed ancestry or if the sample came from a population not included in 1000 G. Such ambiguous samples were labelled as “other”. PC Relate was used to to identify related individuals in NIHR BR-RD. We used the first 20 PCs from PC Air to adjust for relatedness and extracted the pairwise Identity-By-State distances and kinship values. The pairwise information was used by Primus to infer family networks and calculate the maximum set of unrelated samples.
Of the 9110 NIHR BR-RD samples, we assigned 80.2% to Non-Finish European (n = 7307), 7.2% to South Asian (n = 649), 2.3% to African (n = 213), 0.08% to East Asian (n = 78), 0.02% to Finnish-European (n = 19) and 9.2% to Other (n = 844) and retrieved a maximum set of 7,493 unrelated individuals (UWGS10K), representing 82.2% of the entire NIHR BR-RD cohort.
Cohort definition and allele frequency calculation
Based on the relatedness analysis, we defined the following sample subsets: (a) the maximum number of unrelated non-PAH controls (UPAHC, n = 6385), (b) all affected PAH cases (PAHAFF, n = 1048), and (c) all unrelated PAH index cases (PAHIDX, n = 1038). These subsets were used to annotate the variants in the multi-sample VCF file with calculated minor allele frequencies using the fill-tags extension of BCFtools52.
Rare variant filtering
Filtering of rare variants was performed as follows: (1) variants with a MAF less than 1 in 10,000 in UPAHC subjects, UK10K and ExAC were retained (adjusted for X chromosome variants to 1 in 8000); (2) variants with a combined annotation dependent depletion deleteriousness (CADD) score of less than 15 were excluded. CADD scores were calculated using the CADD web service (http://cadd.gs.washington.edu) for variants lacking a score; (3) premature truncating variants (PTVs) or missense variants of the canonical transcript were retained; 4) missense variants predicted to be both tolerated and benign by SIFT and PolyPhen-2, respectively, were removed.
To identify likely causative mutations (as reported in Supplementary Table 3), variants in previously reported and putative genes, identified in this study, were examined in more detail to exclude variants that did not segregate in families (where data available). Furthermore, variants shared between cases and non-PAH controls, as well as variants of uncertain significance that co-occurred with previously reported causative mutations or high impact PTVs were also excluded.
Burden analysis of protein-truncating and missense variants
Filtered variants were grouped per gene and consequence type (predicted PTV/missense) and subjects with at least one variant were counted (no double counting) per group and tested for association with disease. We applied a one-tailed Fisher’s exact test with post hoc Bonferroni correction to calculate the P value for genome-wide significance.
Rare variant analysis using SKAT-O
To further investigate the aggregated effect that rare variants contribute to PAH aetiology, we applied a Sequence Kernel Association test (SKAT-O). SKAT-O increases the power of discovery under different inheritance models by combining variance-component and burden tests. Variants were filtered based on MAF as specified above, and only PTV and missense variants were included. For the analysis we implemented SKAT-O in RvTests v1.9.953 with default parameters and weights being Beta(1,25), and applying a correction for read length, gender and the first five principal components of the ethnicity PCA. Variants were collapsed considering only the protein-coding region in the canonical transcript of the protein-coding genes in the genome assembly GRCh37.
Analysis of large deletions
Copy number variation was identified using Canvas54 and Manta55. Deletions called by both Manta and Canvas with a reciprocal overlap of ≥20% were retained. Of these, deletions were excluded if both failed standard Illumina quality metrics or overlapped with known benign deletions in healthy cohorts56. Deletions with a reciprocal overlap of ≥50% between samples were merged and filtered for a frequency of less than 1 in 1000 in WGS10K and overlapping exonic regions of protein-coding genes (GRCh37 genome assembly). The number of subjects with deletions were added up by gene (no double counting of subjects) and tested for association with the disease. We applied a one-tailed (greater) Fisher’s exact test with Bonferroni post hoc correction for multiple testing to determine the P values for genome-wide significance.
Confirmation of variants
Variant sequencing reads for SNVs, indels and deletions were visualised for validation on Integrative Genomes Viewer (IGV)57, and were confirmed by diagnostic capture-based high-throughput sequencing, if the IGV inspection was not satisfactory. For the familial segregation analysis, linkage to the BMPR2 locus was first examined by microsatellite genotyping analysis. Mutation screening of the BMPR2, ACVRL1, ENG, AQP1 and SOX17 genes was conducted by capillary sequencing using BigDye Terminator v3.1 chemistry. All DNA fragments were resolved on an ABI Fragment Analyzer (Applied Biosystems). All primer sequences are listed in Supplementary Table 9. The family trees were drawn using the R package FamAgg58.
Structural analysis of novel variants
The domain structures and the functional groups of the novel PAH genes were plotted according to the entry in UniProtKB. Clustal Omega was used for sequence alignment. Structural data were obtained from RCSB Protein Data Bank and analysed according to published reports. Figures were generated using PyMOL Molecular Graphics System.
Production of pGDF2 wild type and variant proteins
The cloning of human wild-type pro-GDF2 (pGDF2) in pCEP4 has been described previously59. Site-directed mutagenesis was performed according to the manufacturer’s instructions (QuickChange Site-Directed Mutagenesis Kit, Agilent Technologies). Mutations were confirmed by Sanger sequencing. HEK-EBNA cells were transfected with plasmids containing either wild-type or mutant pGDF2 for 14 h. The transfecting supernatant was removed and replaced with CDCHO media (Invitrogen) for 5 days to express the proteins. The conditioned media containing GDF2 and the variants were collected and snap-frozen on dry-ice before being stored at −80 °C. For each variant, conditioned media from three independent transfections were collected for further characterisation.
High binding 96-well ELISA plates (Greiner, South Lanarkshire, UK) were coated with 0.2 µg/well of mouse monoclonal anti-human GDF2 antibody (R&D Systems, Oxfordshire, UK) in PBS (0.1 M phosphate pH7.4, 0.137 M NaCl, 2.7 mM KCl, Sigma) overnight at 4 °C in a humidified chamber. Plates were washed with PBS containing 0.05% (v/v) Tween-20 (PBS-T), followed by blocking with 1% bovine serum albumin in PBS-T (1% BSA/PBS-T) for 90 min at room temperature. Recombinant human GDF2 standards (1–3000 pg/ml) or conditioned media samples (100 µl/well of 1:30, 1:100, 1:300, 1:1000, 1:3000 and 1:10,000 dilutions) were then added and incubated for 2 h at room temperature. After washing, plates were then incubated with 0.04 µg/well biotinylated goat anti-human GDF2 (R&D Systems) in 1% BSA/PBS-T for 2 h. Plates were washed, then incubated with ExtrAvidin(r)-Alkaline phosphatase (Sigma) diluted 1:400 in 1% BSA/PBS-T for 90 min. Plates were washed with PBS-T followed by water. The ELISA was developed with a colorimetric substrate comprising 1 mg/ml 4-Nitrophenyl phosphate disodium salt hexahydrate (Sigma) in 1 M Diethanolamine, pH 9.8 containing 0.5 mM MgCl2. The assay was developed in the dark at room temperature and the absorbance measured at 405 nm.
Cell culture and treatments
Distal human pulmonary artery smooth muscle cells (PASMCs) were cultured from explants dissected from lung resection specimens. Small pulmonary arterioles (0.5 to 2 mm diameter) were dissected and divided into small pieces before plating in T25 flasks. Explants were left to adhere for 2 h and then incubated in DMEM/20% FBS plus amino acids at 37 °C in 95% air/5% CO2 until PASMCs had formed confluent monolayers. Cells were then trypsinized, and for subsequent passages cells were maintained in DMEM supplemented with 10% FBS. The cellular phenotype of PASMCs was confirmed by positive immunofluorescence staining with anti-smooth muscle specific alpha-actin (Clone IA4 Sigma-Aldrich; 1:100 dilution). The derivation of human tissues and cells was approved by Papworth Hospital ethical review committee (Ref 08/H0304/56 + 5) and all subjects provided informed and written consent.
Human blood outgrowth endothelial cells (BOECs) were derived from 40–80 ml of peripheral venous blood isolated from healthy subjects. The study was approved by the Cambridgeshire 3 Research Ethics Committee (Ref 11/EE/0297), and all subjects provided informed and written consent. BOECs were cultured in 10% FBS supplemented with EGM-2MV (Life Technologies, Carlsbad, CA). Cells were used between passages 4 and 860. The endothelial phenotype of BOECs was determined by flow cytometry for expression of endothelial surface markers, as described previously25. Cells were routinely tested to exclude mycoplasma infection.
Human pulmonary artery endothelial cells (PAECs) were purchased from Lonza (Cat. No. CC-2530; Basel, Switzerland). Cells were maintained in EGM-2 with 2% FBS (Lonza). PAECs were used for experiments between passages 4 and 8. For experiments cells were cultured in the presence of EBM-2 containing Antibiotic-Antimycotic (Invitrogen, Renfrewshire, UK). Cells were routinely tested to exclude mycoplasma contamination.
RNA preparation and quantitative reverse transcription-PCR
Total RNA was extracted using RNeasy Mini Kit with DNAse digestion (Qiagen, West Sussex, UK), according to the manufacturer’s instructions. cDNA was prepared from 1 µg of RNA using High Capacity Reverse Transcriptase kit (Applied Biosystems, Foster City, CA). Quantitative PCR reactions employed MicroAmp optical 96-well reaction plates (Applied Biosystems). 50 ng µl−1 cDNA was used with SYBR Green Jumpstart Taq Readymix (Sigma-Aldrich), ROX reference dye (Invitrogen) using custom made sense and anti-sense primers (all 200 nmol l−1). Primers for human ACTB (encoding β-actin), AQP1, ATP13A3, B2M, HPRT and SOX17 were designed using PrimerBLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/; Supplementary Table 9). Reactions were amplified on a Quantstudio 6 Real-Time PCR system (Applied Biosystems). The relative abundance of each target gene in different cell lines was compared using the equation 2−(CtGOI-Ct3HK), where Ct3HK corresponded to the arithmetic mean of the Cts for ACTB, B2M and HPRT for each sample. For expression analysis of siRNA knockdown, the 2−(ΔΔCt) method was used and fold expression determined relative to the DH1 control.
Prior to transfection, cells were preincubated in Opti-MEM-I reduced serum media (Invitrogen) for 2 h before transfection with 10 nM siRNA that had been lipoplexed for 20 min at RT with DharmaFECT1 (GE Dharmacon, Lafayette, CO). Cells were then incubated with the siRNA/DharmaFECT1 complexes for 4 h at 37 °C before replaced by full growth media. Cells were kept in growth media for 24 h before further treatment. Knockdown efficiency was confirmed by mRNA expression or immunoblotting. For proliferation assays, parallel RNA samples were collected both on day0 and day6, confirming that ATP13A3 expression was reduced by >90% on Day 0 and still reduced by >70% at Day 6. For all other assays, parallel RNA samples were collected on the day of the experiment to confirm knockdown, which was >90%. The siRNAs used were oligos targeting ATP13A3 (SASI_Hs02_00356805) from Sigma-Aldrich and ON-TARGET plus non-targeting Pool (siCP; GE Dharmacon).
Flow cytometric apoptosis assay
BOECs were plated 150,000/well into 6-well plates and transfected with siATP13A3 or siCP lipoplexed with DharmaFECT1. Cells were then serum-starved in EBM-2 (Lonza) containing 0.1% FBS and A/A for 8 h before treating with EBM-2 and A/A containing either 0.1%FBS or 5%FBS for another 24 h. Cells were then trypsinized and after washing with PBS, stained using the FITC Annexin V Apoptosis Dectection Kit I (BD Biosciences). For each condition, dual-staining of 5 µl FITC conjugated Annexin V and 5 µl propidium iodide (PI) were added and incubated at room temperature for 15 min. For the single staining controls for compensation, either 5 µl FITC Annexin V or 5 µl PI was added into non-transfected cells. All samples were analysed on BD Accuri™ C6 Plus platform (BD Biosciences). Data were collected and analysed using FlowJo software, with AnnexinV+/PI− cells defined as early apoptotic (Treestar).
Caspase-Glo 3/7 assay
BOECs were seeded at a density of 150,000/well into 6-well plates and transfected with siATP13A3 or siCP lipoplexed with DharmaFECT1. For each condition, cells were trypsinized from 6-well plates and reseeded in triplicates into a 96-well plate at a density of 15,000–20,000/well and left to adhere overnight. Cells were quiesced in EBM-2 containing 0.1% FBS for 24 h before treating with or without EBM-2 and A/A containing either 0.1% FBS or 5% FBS for 16 h. For measuring caspase activities, 100ul Caspase-Glo® 3/7 Reagent (G8091 Promega) was added into each well, incubated and mixed on a plate shaker in the dark for 30 min at room temperature. The lysates were transferred to a white-walled 96-well plate and luminescence was read in a GloMax® luminometer (Promega).
WGS data of PAH cases included in this manuscript and eligible for public release according to the UK Research Ethics rules have been deposited in the European Genome-phenome Archive (EGA) at the EMBL—European Bioinformatics Institute under accession number EGAD00001003423.
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The UK National Institute for Health Research BioResource—Rare Diseases (NIHR BR-RD) and the BHF/MRC UK National Cohort of Idiopathic and Heritable PAH made this study possible. We gratefully acknowledge the participation of patients recruited to the NIHR BR-RD. We thank the NIHR BR-RD staff and co-ordination teams at the University of Cambridge, and the research nurses and coordinators at the specialist pulmonary hypertension centres involved in this study. The UK National Cohort of Idiopathic and Heritable PAH is supported by the NIHR, the British Heart Foundation (BHF) (SP/12/12/29836), the BHF Cambridge Centre of Cardiovascular Research Excellence, the UK Medical Research Council (MR/K020919/1), the Dinosaur Trust, BHF Programme grants to R.C.T. (RG/08/006/25302) and N.W.M. (RG/13/4/30107), and the UK NIHR Cambridge Biomedical Research Centre. Funding for whole-exome sequencing was provided through a Bart’s Charity award (MGU0205) to R.C.T. and D.v.H. N.W.M. is a BHF Professor and NIHR Senior Investigator. CH is a NIHR Rare Disease Translational Research Collaboration Clinical PhD Fellow. L.S. is supported by the Wellcome Trust Institutional Strategic Support Fund (204809/Z/16/Z) awarded to St. George’s, University of London. C.J.R. is supported by a BHF Intermediate Basic Science Research Fellowship (FS/15/59/31839). A.L. is supported by a BHF Senior Basic Science Research Fellowship (FS/13/48/30453). We acknowledge the support of the Imperial NIHR Clinical Research Facility, the Netherlands CardioVascular Research Initiative, the Dutch Heart Foundation, Dutch Federation of University Medical Centres, the Netherlands Organisation for Health Research and Development and the Royal Netherlands Academy of Sciences. We also gratefully acknowledge Dr Claudia Cabrera in the NIHR Barts Cardiovascular Biomedical Research Centre for bioinformatics support. We thank all the patients and their families who contributed to this research and the Pulmonary Hypertension Association (UK) for their support.