Fig. 10 | Nature Communications

Fig. 10

From: Identification of rare sequence variation underlying heritable pulmonary arterial hypertension

Fig. 10

Functional studies of novel genes. ac Expression of a AQP1, b ATP13A3 and c SOX17 mRNA in human pulmonary artery smooth muscle cells, pulmonary artery endothelial cells and blood outgrowth endothelial cells (BOECs) (n = 4 biological replicates of each). Relative expression of each transcript was normalised to three reference genes, ACTB, B2M and HPRT. d Proliferation of BOECs in 5% FBS over 6 days. Cells were transfected with DharmaFECT1 alone (DH1), siATP13A3 or non-targeting siRNA control (siCP) e, f Quantification of apoptosis in BOECs, defined as Annexin V+/PI− cells, in BOECs transfected with siATP13A3 or siCP in complex with DH1 followed by 24 h treatment with 0.1% FBS or 5% FBS (n = 4 biological repeats). f Measurement of apoptosis via Caspase-Glo 3/7 activity measurements in BOECs transfected with siATP13A3 or siCP in complex with DH1, followed by 16 h treatment in 0.1% FBS or 5% FBS. Data are from a single experiment (n = 4 wells) representative of 3 biological repeats. Data were analysed using a One-way analysis of variance with post hoc Tukey’s test for multiple comparisons in d and f. Data were analysed using a repeated measures One-way analysis of variance with post hoc Tukey’s for multiple comparisons in e. *P < 0.05, **P < 0.01 within treatment groups. ###P < 0.001 for effect of ligand against control for same transfection condition

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