Fig. 4 | Nature Communications

Fig. 4

From: RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape

Fig. 4

RodZ binding and MreB mutations may alter the bending properties of MreB filaments. a Schematic of an MreB dimer (PDB ID: 1JCG) and its orientation relative to the membrane. The bending of MreB protofilaments is captured by the relative orientation along three orthogonal axes (cylinders) of adjacent MreB subunits, colored light (top) and dark gray (bottom). The membrane binding interface of the MreB protofilament is shown as a green plane. b Schematic of MreB dimer bending angles out of the plane of the membrane (θ1, top) and in the plane of the membrane (θ2, bottom). In the crystal structures that form the initial states of our MD simulations, these bending angles are zero. c MD simulation system comprised of a nucleotide-bound T. maritima MreB dimer, with each subunit bound to the cytoplasmic tail of RodZ. d D72E, R112C, A162T, and E266D mutations in ATP-bound T. maritima MreB shift the θ1 bending angles toward that of a RodZ-bound ATP dimer, signifying filament straightening

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