Fig. 1 | Nature Communications

Fig. 1

From: Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion

Fig. 1

Expression of SALL4, miR-200c, and PD-L1 in the center tumor regions of HCC. a Predicted microRNA targeting PD-L1 3´-UTR includes miR-200a, miR-200c, and miR-383 (left); the upstream promoter region of the human miR-200c showing putative SALL4-binding sites (right). b Immunostaining of SALL4 and PD-L1 proteins (brown color) and in situ hybridization for hsa-miR-200c (blue purple color) in the center of the tumor (CT) and peritumor (PT) regions of HCC. Original magnifications: × 10, × 20; bar = 100 µm, 50 µm, respectively. ce Increased SALL4 (c, P < 0.0001), PD-L1 (d, P < 0.0001) and miR-200c expression (e, P < 0.0001) in CT regions. Cumulative data calculated by two-tailed unpaired Student’s t-test. The results are expressed as the mean ± SEM. ****P < 0.0001. f Disparity analysis between miR-200c and PD-L1 (left), miR-200c and SALL4 (middle), PD-L1 and SALL4 (right) in CT and PT regions. nP, number of patients with PD-L1; nm, number of patients with miR-200c; nS, number of patients with SALL4

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