Fig. 5 | Nature Communications

Fig. 5

From: Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis

Fig. 5

Positive-inside rule tally for BacA. a Sequence of BacA from E. coli rainbow color coded from N-terminus to C-terminus. Helices observed in the crystal structure are numbered as described in the text. b Distribution of cationic residues arginine and lysine in the periplasmic and cytoplasmic exposed stretches of polypeptide. Arginines and lysines on the cytoplasmic side considerably outnumber those on the periplasmic side of the membrane suggesting the protein is oriented with its N-terminus and C-terminus in the periplasm. c The table enumerates the relevant amino acids on either side of the membrane. d Overall architecture of BacA indicating the cationic residues, as described in b, as magenta spheres. e Surface representation of BacA where cationic (Arg, His, Lys) and anionic (Asp, Glu) residues are colored blue and red, respectively. H4 and H8 are colored green and orange, respectively. All other helices are colored gray

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