Fig. 7 | Nature Communications

Fig. 7

From: Atrx inactivation drives disease-defining phenotypes in glioma cells of origin through global epigenomic remodeling

Fig. 7

GNA13 promotes cell migration in ATRX-mutant GSCs. a, b western blot (a) and RT-qPCR (b; three replicates) showing increased GNA13 expression in IDH1-mutant (IDH1 R132H), ATRX-deficient GSC line (GS 5–22). c transwell migration assay (5 replicates) demonstrating increased motility in IDH1-mutant, ATRX-deficient GSC line (GS 5-22) relative to IDH1-mutant, ATRX-intact GSC line (TS 603). de western blot (d) and RT-qPCR (e; three replicates) showing decreased Gna13 expression upon transient transfection of Atrx- mNPCs with human ATRX (hATRX) relative to control vector (Con). f transwell migration assay (5 replicates) demonstrating decreased motility in hATRX transfected, Atrx- mNPCs. hi western blot (h) and RT-qPCR (i; three replicates) showing decreased GNA13 expression upon transient transfection of GS 5-22 cells with hATRX relative to Con. j transwell migration assay (5 replicates) demonstrating decreased motility in hATRX transfected GS 5-22 cells. All western blots used Vinculin as a loading control. In all cases, error bars reflect standard deviation and P values determined by unpaired two-tailed t-test

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