Fig. 6 | Nature Communications

Fig. 6

From: Atrx inactivation drives disease-defining phenotypes in glioma cells of origin through global epigenomic remodeling

Fig. 6

Gna13 promotes cell migration in Atrx- mNPCs and is upregulated in ATRX-deficient gliomas. a schematic demonstrating strategy to identify upregulated genes promoting mNPC motility in the setting of Atrx deficiency (FC: fold change). b relative mNPC transwell migration (upper chart) following siRNA knockdown (25 nM) of the indicated gene target (lower panel), as confirmed by RT-qPCR (error bars reflect standard deviation; P values determined by unpaired two-tailed t-test). c, d LGG expression data from TCGA showing levels of ATRX and GNA13 expression in ATRX-intact (ATRX+) and ATRX-deficient (ATRX-) gliomas. Error bars reflect the 10–90 percentile spread and P values determined by unpaired two-tailed t-test. e micrographs of ATRX+ and ATRX- gliomas showing differential GNA13 expression by IHC (hematoxylin counterstain; scale bar 100 µm)