Diatoms are one of the major primary producers in the ocean, responsible annually for ~20% of photosynthetically fixed CO2 on Earth. In oceanic models, they are typically represented as large (>20 µm) microphytoplankton. However, many diatoms belong to the nanophytoplankton (2–20 µm) and a few species even overlap with the picoplanktonic size-class (<2 µm). Due to their minute size and difficulty of detection they are poorly characterized. Here we describe a massive spring bloom of the smallest known diatom (Minidiscus) in the northwestern Mediterranean Sea. Analysis of Tara Oceans data, together with literature review, reveal a general oversight of the significance of these small diatoms at the global scale. We further evidence that they can reach the seafloor at high sinking rates, implying the need to revise our classical binary vision of pico- and nanoplanktonic cells fueling the microbial loop, while only microphytoplankton sustain secondary trophic levels and carbon export.
The widely accepted characteristics of spring blooms in marine systems are that highly turbulent front regions and eutrophic areas generally result in the proliferation of diatoms along an ecological succession sequence, which then favors coccolithophores and finally dinoflagellates as stratification and oligotrophy increase1. Another textbook view of the functioning of these high primary productivity events is that they contribute disproportionately to the export of carbon to the ocean interior. This concept is explicit in Legendre and Lefèvre’s bifurcation model2, which states that large microalgal blooms often result in increased sinking of phytoplanktonic cells or fecal pellet production, while smaller organisms are preferentially shifted toward the microbial loop, thereby reducing the fraction available for export2. Phytoplankton communities are typically characterized using bulk measurements, satellite data and model outputs that do not yet allow a fine scale understanding of specific floristic successions. Since the nature of the organisms composing the bloom events has dramatic effects on both higher trophic levels and biogeochemical export fluxes, it is crucial to refine our understanding of their succession determinism.
For eutrophic regions, a three-stage typical spring bloom diatom succession was proposed by Margalef1 and modified by Guillard and Kilham3. It involves a first sequence after upwelling or strong mixing that is dominated by fast-growing small species (>10 µm) belonging to the Thalassiosira, Chaetoceros, or Skeletonema genera, followed next by the appearance of a larger number of medium-sized Chaetoceros species, often forming long chains. Last, as nutrients are consumed, species more adapted to oligotrophic environments then thrive, such as large Rhizosolenia and Hemiaulus species, often associated with nitrogen-fixing cyanobacteria. The rate of succession can then be modulated by loss rates of diatoms from the euphotic zone, through diffusion, sinking, and grazing3. At the global scale, centric diatoms such as Rhizosolenia, Chaetoceros, and Thalassiosira represent a little under 50% of total diatom biomass4, tending to confirm this general diatom succession. However, deviations from Margalef’s typical diatom bloom scenario1, with very-small (<5 µm) diatom species developing into quasi-monospecific blooms have been reported on occasion, mostly in mid- and high-latitude well-mixed environments5,6,7,8,9,10. These phenomena are depicted as anomalous, and as a consequence, the large-scale distribution and significance of these minute diatom species are still not widely recognized.
Here, we present data relative to the 2013 spring bloom in the Northwestern Mediterranean Sea, evidencing the massive development of the small diatom genus Minidiscus, which overlaps both the pico- and nano-size-fractions with diameters ranging from <2 to 5 µm. We show that this tiny diatom accumulated in extremely high numbers (106cells L−1) over a deep convection area following a particularly intense winter mixing event which extended down to the sea bottom (2400 m)11. Thanks to a trait-based modeling approach, we attempt to determine which key biological factors favor the proliferation of such nano-sized diatom blooms and propose that top-down control mechanisms (selective grazing, viral or bacterial lysis) may exert a specific control on the development of larger cells, opening an ecological niche for small diatoms to succeed. We then extend our results to a global scale, using metabarcoding data from the Tara Oceans survey12, showing that Minidiscus ranked in the top 20 most abundant diatom genera, although it has rarely been described in phytoplankton process studies. We propose, as hinted by several other authors, but still not integrated into the classical view, that nanoplanktonic diatom blooms may be more frequent than currently appreciated in open ocean and coastal areas perturbed by turbulence following vernal mixing or frontal stirring, but that their tiny dimensions and dynamics have prevented both adequate sampling and observation until now. Last, we look for evidence of the potential impact of these diatoms in carbon export, using our Mediterranean case study data from Tara Oceans and from literature reviews, and question whether we should revise the classical view that lower-end nanoplankton-sized cells are entirely recycled through the microbial loop and do not contribute in any significant way to carbon export to the deep ocean, as was already suggested for picophytoplankton13. We thus highlight these tiny, elusive diatom species as being of potential global significance in productive environments and for carbon export.
Spring bloom of nano-sized diatoms in the Mediterranean Sea
The Gulf of Lions in the northwestern Mediterranean Sea is the hotspot for the recurrent deep-water formation during winter, due to significant autumnal and winter heat losses caused by strong Northern winds, and the presence of a cyclonic gyre enclosing dense water in the middle of the basin14. An intense convection event occurred during the Deep Water Experiment (DeWeX) in 2013, leading to mixing of the water column from the surface to the seafloor at 2400 m depth15. This massive overturn induced fertilization of the surface waters with upwelled nutrients and triggered a large phytoplankton spring bloom (Fig. 1a, b). Such an annual event is well known and recurrently observed, both from space16,17 and from field campaigns18. However, the nature of the phytoplankton bloom in this area is poorly characterized, even though it has been estimated to contribute ~15% of the primary production in the Mediterranean Sea16.
In such a turbulent eutrophic environment, small- to medium-sized chain-forming diatom genera are usually expected to proliferate first upon alleviation of light limitation, followed by larger species as nutrients are consumed. However, phytoplankton determination during the DeWeX spring bloom revealed a different diatom community, with a massive accumulation dominated by at least two species of the smallest known centric diatom genus Minidiscus (M. trioculatus and M. comicus). They belong to the very-small end of the nanophytoplankton size-fraction (~2–5 µm diameter in our observations) even though they are reported to extend to the pico-size-fraction with known minimum diameters for M. trioculatus and M. comicus of 1.5 and 1.9 µm, respectively19. These species reached very-high abundances (4–6 million cells L−1 at several stations in April—Leg 2) and were the dominant diatoms over a large region (17 out of 32 stations) of the study area (Fig. 2a, b and Supplementary Figs. 1 and 2). Their abundance peaked mainly in the center of the convection region at around 42°N, 5°E and in an anticyclonic eddy south of the study region around 40.5°N, 6°E, where abundances ranged from 5000 to 425,000 cells L−1. Meanwhile, microphytoplanktonic diatoms (>20 µm) were only observed closer to the Gulf of Lions plateau in winter and in the northeastern region between the coast of France and Corsica in spring, but never exceeded 17,000 cells L−1, a low value for a spring bloom. Minidiscus was not observed at any site during February (leg 1), which suggests an earliest bloom initiation in March. Increased biogenic silica (BSi) in the areas where Minidiscus was absent was associated with typical larger diatoms such as Pseudo-nitzschia spp., Leptocylindrus spp., Cylindrotheca spp., and other large Thalassiosira spp. during winter and mainly to Guinardia delicatula and Chaetoceros spp. during spring (Supplementary Figs. 1 and 2). At a few stations during April (leg 2), the small pennate Nitzschia bicapitata (~10 µm) was also observed in elevated concentrations in association with Minidiscus, but the abundances of the latter were always at least 1 or 2 orders of magnitude higher. BSi concentrations associated with the densest Minidiscus bloom area were elevated for the Mediterranean Sea (1 µmol L−1) and particulate Si:C ratios (0.07–0.10) were close to Brzezinski’s values for small diatoms20. An estimation of the growth rates required to produce such an accumulation yields a minimum approximate net rate of 0.3 d−1 (for an average of 805,000 cells L−1) between early March and mid-April starting from a seed population of 10–100 cells L−1. This is higher than the rate of 0.13 d−1 measured in situ during a more moderate Minidiscus spring bloom observed in the Norwegian basin in 201210, which could be explained by the lower temperature (~6–7 °C) compared to our study area (~13 °C)21. However, if the Minidiscus bloom grew over a shorter period of time, for instance over a week, growth rates would have been much higher and closer to 2 d−1. The unexpected very-small size structure of this phytoplankton community was confirmed by an automated flow cytometer installed on an inflow of surface water pumped continuously at 3 m depth. It evidenced that the massive spring bloom in April was almost entirely dominated by nanophytoplankton (Supplementary Fig. 3). Observations by optical microscopy revealed that the Minidiscus bloom co-existed with even larger numbers of undetermined nanoflagellates and cryptophytes, all smaller than 20 µm, while abundances of larger cells were about three orders of magnitude lower at most sites (Supplementary Fig. 3).
A further unusual feature was the overall dominance of diatoms by Minidiscus during this bloom. At all sites where Minidiscus was observed, it represented on average 92% of total diatom abundance (Table 1). Notwithstanding, conclusions drawn on abundance are difficult to transpose to biogeochemical relevance, in particular at both ends of the size spectrum, where abundance and biomass are no longer closely related. To assess whether this numerically abundant bloom was an important contributor to total phytoplankton biomass, we converted abundance data to carbon (C) content, using an average biovolume of 18 µm3 and subsequent Si and C content estimate of ~0.8 pmol C cell−1 and 0.08 pmol Si cell−1, consistently with the data given in Brzezinski (1985)20 for another similar sized species (24 µm3), but about 3-fold higher than the quotas predicted using standard allometric approaches22,23. Minidiscus biomass was then compared to measured particulate organic carbon (POC) and BSi concentrations in the same samples. Although Minidiscus was not the dominant contributor to C biomass given its small size, the high abundances of other nanoplankton groups and the variable content of dead material in POC, it still reached between 18 and 26% of total POC at stations 74 and 99 (Table 1), located close to the center of the convection area (42°N, 5°E). At station 74, Minidiscus constituted the bulk of in situ BSi stocks (99.4%) on one occasion on April 19th, whereas its relative contribution decreased to 36% by the end of April at the same location (Table 1).
This locally significant contribution is surprising given that the genus Minidiscus has never been documented to form such intense blooms in the Mediterranean Sea. Minidiscus is in fact absent from many taxonomic books for this basin, and appeared for the first time in an inventory of the Catalan Sea only in 199224. M. comicus was only recently observed in the same region of the northwestern Mediterranean during spring25, and in nearby regions of the Gulf of Naples26, while M. trioculatus was considered to be rare. Minidiscus was observed for the first time in April 2012 at a coastal bi-monthly time-series site located on the Gulf of Lions coastal shelf in the Bay of Marseille (SOMLIT), at very low baseline abundances. Higher abundances were reported in May and July 2013 following our study (B. Beker, personal communication). The only other report of such massive nano-sized diatom bloom in the Mediterranean Sea was that of the small centric diatom Thalassiosira partheneia (<8 µm) in the Almeria-Oran front6, where it accumulated in a thin layer at depth up to ~10 million cells L−1 with a similar calculated net growth rate of 0.2 d−1.
The nutrient concentrations at stations sampled during winter revealed that the amount of nutrients upwelled to the euphotic layer was dependent on the area covered by the convection event, while nutrient stoichiometry was dependent on the vertical extent of the convection depth11. From a hierarchical ascendant classification analysis of stations sampled during winter (DeWeX cruise leg1), we found that highly convective regions were characterized in the surface layer (0–50 m) by high H4SiO4 and NO3− concentrations (7.7 µM and 8.4 µM, respectively), while weakly convective regions showed much lower nutrient content (2.1 µM Si and 2.7 µM N, respectively)11. In parallel, the Si:N nutrient ratio prior to the spring bloom in the surface layer was 15% higher in the case of high convection compared to low convection or no mixing at all (Fig. 3). High convection events mixing deep water masses with the surface layer thus seem necessary to supply enough Si to the surface relative to N and P to sustain a high bloom situation dominated by diatoms27. The dominance of Minidiscus during DeWeX could thus partly be attributed to their higher efficiency with respect to larger diatoms for taking up high silicic acid concentrations, which were preferentially upwelled compared to nitrate28. In the absence of physiological rate measurements during the cruise, we rely on the following modeling exercise (not coupled to physical forcings) to test which bottom-up or top-down processes could best explain the preferential development of nano-sized diatoms over larger species.
Biogeochemical models are often based on two main numerical representations of biota, the plankton functional type (PFT) models that are now widely used29 and the trait-based models grounded on the Reynolds’ C-S-R classification30 built on Margalef’s mandala1. On one hand, PFT-based models only depict diatoms as a single large phytoplankton box connected to the Si cycle and are, therefore, inherently unable to reproduce different life strategies and traits within diatom community. On the other hand, the C-S-R models can be applied in the present context as an adapted tool to represent a spectrum of different strategies of development among diatoms. Each of these strategists is characterized by a specific ecological niche, defined by a combination of nutrient and light variables, which are the major factors impacting diatom survival strategies31. We used a simple C-S-R trait-based model representing four different types of strategists to attempt to understand the reasons underlying the occurrence of the massive bloom of Minidiscus evidenced during DeWeX in 2013. Among the four considered strategists, two diatom types were differentiated. Owing to its morphological and physiological traits, Minidiscus was considered in the present model as a colonist genus (i.e., a C-strategist). This group is characterized by a high growth rate, a small size and a round shape (Figs. 4 and 5). The other diatom type, characterized by higher growth rates, larger sizes and elongated shapes, such as Chaetoceros, groups light-stress tolerant ruderal species (i.e., R-strategists). Using conditions based on the measured light and nutrient levels prevailing prior to the 2013 spring bloom27, the results of our trait-based model show a large and rapid dominance of R-strategist diatoms (Supplementary Fig. 4 and 5), which is not in line with the observations of the Minidiscus bloom. Given the nature of our model, this result suggests that some processes occurring in the field other than bottom-up factors may prevent the bloom of R-strategists, such as for instance a sharp increase in their mortality rate, which in turn may favor the emergence of C-strategists (i.e., Minidiscus) by escaping predation. When higher mortality rates are applied to the R-strategists, the model correctly simulates a rapid dominance of C-strategists. Although the numerical model is unable to determine which exact process is driving the prevalence of C-strategists over R-strategists in the field, it nonetheless offers a plausible hypothesis that a selective top-down control on larger diatoms is necessary given each strategist’s defined niche and environmental parameters matching the DeWeX dataset. In situ, viral lysis, attacks by various pathogens32, differential sensitivity to mixing, preferential grazing as well as larger differences in the traits between Minidiscus and others diatom genera are some of the known processes that may exert control on abundance of large diatoms. A transient lower vulnerability or accessibility of Minidiscus to grazers may also be an indirect mechanism involving its success during DeWeX. It is known that Minidiscus can produce some protruding organic threads depending on turbulence conditions33 and that these threads would increase its vulnerability to copepod grazing34. Unfortunately, these delicate threads are only preserved up to three months in glutaraldehyde and dissolve in less than 15 days in Lugol34, which made us unable to confirm their presence. The upscaling of mortality rates for large diatoms in the model is further supported by the observations made on the zooplankton community during DeWeX35. The deep convection zone where Minidiscus prevailed was characterized by high abundances of large herbivorous genera (such as Centropages, Calanus) while smaller grazers (e.g., Microsetella, Oncae) were much less abundant. Trophic pathways of phytoplankton community through the zooplankton food-web determined using stable isotopes during DeWeX, also revealed that the nanoplankton size-class made the largest contribution to zooplankton biomass during winter, while the high convective area was characterized by the largest contribution of microplankton to zooplankton biomass during spring36. Hence, an ecological framework for nanoplanktonic diatom blooms can be postulated from this simple trait-based model and supported by observation. Small diatoms would be likely dominant during the early bloom phase in both low and high convective areas but in the latter case only a strong top-down control on R-strategists (by viral/bacterial pathogenesis, parasites or grazing) would allow nano-sized species, such as Minidiscus to reach the observed high bloom intensities.
The case for large-scale oversight of nano-diatoms
The genus Minidiscus, composed of only a few reported species, was initially described in 1973 in the Norwegian Sea37. It is considered a cosmopolitan genus38, having been observed in all oceanic basins, including northern and southern polar environments (Fig. 6). However, this genus goes frequently unnoticed because it is easily overlooked in conventional microscopy, and is often misidentified and/or systematically under-sampled by net hauls due to inappropriate mesh sizes. When discrete water sampling is adequate, pico- and nano-sized diatoms may be enumerated using light microscopy but can only be determined to the genus and species levels using scanning electron microscopy (SEM) (Fig. 5), a difficulty that has already been emphasized for Minidiscus9,38,39,40. Only a few other studies have documented significant bloom events of nano-sized diatoms. Blooms of Minidiscus spp. have for instance been reported during a 14-year survey in Monterey Bay, a coastal region characterized by strong upwelling events, an ecological situation similar to that of the DeWeX study9, as well as in other eutrophic areas of the Subarctic Pacific5, Norwegian Basin10, and Antarctic Peninsula39,41. In the Mediterranean Sea, a massive bloom (~10 million cells L−1) of the nano-sized diatom Thalassiosira partheneia (<8 µm) was reported near the Gibraltar Strait6. Large spring blooms of the tiny pennate diatom Nanoneis hasleae (2 × 5 µm) have also been reported on a few occasions in the North Atlantic and may be similarly overlooked on a global scale42,43. Nanoplanktonic biomineralizing algae, including diatoms, Parmales (siliceous plate-bearing phytoplankton <5 µm) and coccolithophores were also found to have been largely underestimated in the Southern Ocean44.
It, therefore, seems possible that Minidiscus together with other nanoplanktonic sized diatoms, may be responsible for occasional massive blooms, which may go undetected due to either collection or identification biases. This is further exemplified in the global diatom database compiled during the MAREDAT project4, which presented close to 10,000 unique georeferenced locations and 607 reported diatom species since the 1930 s, in which nano-sized genera such as Minidiscus, Minutocellus, Cyclotella, Lennoxia,or Nanofrustulum are completely absent. This absence suggests that, if adequately sampled at all, they were either confused with similar looking species such as small Thalassiosira species, counted as undetermined species, or simply not even recognized as diatoms.
Tara Oceans metabarcoding data
The advent of high-throughput sequencing now allows unprecedented access to pico- and nano-plankton, as they can be detected even at low levels in filtered samples, thus circumventing both sampling and observational biases. Analysis of the metabarcoding-based descriptions of eukaryotic plankton by Tara Oceans45,46 indeed reveals the predominance of nano-sized diatoms such as Minidiscus (Fig. 7) and Minutocellus (Supplementary Fig. 6). Their ubiquitous biogeographical distribution confirms and significantly extends previous observations, in particular regarding open-ocean systems, as previous biogeography described in the literature evidenced Minidiscus mostly in coastal environments (Fig. 6). Significantly, Minidiscus is in the top 20 most abundant diatom genera (Supplementary Fig. 7) even though it has never been described as a major bloom-forming species46. Supporting our previous observations made during DeWeX, the distributions from the Tara Oceans data show that the relative abundance of Minidiscus was the highest in the Mediterranean Sea, followed by the Southern Ocean and the North Atlantic Ocean (Supplementary Fig. 8a). Furthermore, the data reveal that Minidiscus is not only abundant at the surface but also in samples taken from deep chlorophyll maxima (DCM) contributing to what is known as the shade flora which benefits from the best ratio between sufficient light and upward limiting nutrient fluxes (Supplementary Fig. 8b). Size-class fractionations further confirmed that Minidiscus and Minutocellus were mostly represented in the smaller size-fractions, but also occurred in larger size classes probably due to cell aggregation (Supplementary Fig. 8c). A recent study has also transferred two Thalassosira species to the Minidiscus genus after fine structure examination and molecular sequence comparisons47, illustrating the difficulty of correct identification in this size-class. These recent changes have been included in the present Tara Oceans data analysis, but do not significantly change the global scale picture. However, while sequence information is available from M. trioculatus cultures, this is not yet the case for M. comicus implying that Tara Oceans data underestimate Minidiscus abundances. Also recently, Minutocellus has been shown to be a symbiotic species of the foraminifera Pararotalia calcariformata in the Mediterranean Sea48, which is another factor explaining why they may be overlooked in phytoplankton samples and why they are more important in the large size-fraction of the Tara Oceans samples than Minidiscus (Supplementary Fig. 7c). These results, together with previous observations, build a strong case for a large-scale oversight of these genera, together with many other nano-sized diatom species in the marine realm.
Contribution of nanoplanktonic diatoms to carbon export
In order to examine the impact of small-sized diatoms to carbon export to depth, we collected samples from a deep sediment trap (2400 m) moored at 42°N, 4.5°E from the end of March to the end of May 2013 during the DeWeX study, below the main area where Minidiscus reached ~6 million cells L−1. SEM observations of samples from the three sediment trap samples collected during that period revealed the presence at high abundances of both M. trioculatus and M. comicus frustules (Table 2 and Fig. 5e–h). Due to the low representativity of SEM counts (small area imaged and heterogeneous nature of the collected material), accurate Minidiscus flux calculations were not attempted. However, given the time-lag between surface observations and sediment trap collection of Minidiscus, about one month assuming a bloom initiation in early March, a rough estimate indicates that a minimum sinking rate of 80 m d−1 is likely, which is in the range of literature values for phytoplankton aggregates49,50. It was also inferred from the vertical distribution and temporal evolution of particle concentrations in the water column during the DeWeX cruises51 that particulate matter was primarily composed of aggregates with settling rates of ~1 mm s−1 (i.e., 86 m d−1), which supports the previous hypothesis of a rapid sinking of Minidiscus as phytoplanktonic aggregates. Unidentifiable clumped aggregates were observed in SEM, but could not be discriminated with certainty between fecal pellets or phyto-aggregates due to the long-term storage of these samples between sampling and observation (4 years). It was already noted using culture collections of M. trioculatus that single cells growing exponentially became aggregated in compact clusters during stationary phase40. It is further known that these species are able to grow thin chitin threads protruding from the strutted processes of the valves, a feature considered unique to Thalassiosiroids52 and modulated by turbulence33. Finally, M. comicus is known to form small colonies of 2–3 cells connected with extruded threads40. Clearly, these features could if present, increase aggregation and sinking rates significantly33,34.
At least three other studies carried out at both northern and southern high latitudes have revealed high abundances and even a dominance of Minidiscus in sediment traps. The first study was carried out in relatively shallow sediment traps (100–200 m) during a temporal survey from 1985 to 1987 in a Vancouver Island inlet where abundant contributions of both M. chilensis and M. trioculatus were noted in spring and winter53. Other studies carried out near the Antarctic Peninsula reported high abundances of M. chilensis in surface waters39,41, and the annual survey conducted in 1998 in the Bransfield Strait revealed massive M. chilensis fluxes in 1000 m deep trap samples, representing up to 87% of total diatom flux and associated to elevated BSi flux (~150 mg Si m−2 d−1)39.
In support of the hypothesis of a rapid sinking of nano-diatoms, Tara Oceans data show that both Minidiscus and Minutocellus cells are consistently present in multiple mesopelagic (MESO) samples collected at around 700 m depth (Supplementary Fig. 8c) while, on average, diatoms represent up to 46% of photosynthetic read abundance at 700 m. Furthermore, with respect to samples collected in the photic zone, these small diatoms are present at higher proportions with respect to other diatom genera, e.g., Minidiscus is the eighth most abundant diatom genus in MESO samples and the 21st most abundant in photic zone samples (Supplementary Fig. 7). The conclusion from these multiple observations is therefore that small nano-sized diatoms are able to contribute to deep-sea carbon injection, even though this compartment is not usually considered to fuel export.
Minidiscus was described for the first time in 197337 and has traditionally been considered a rare genus, but the results reported here show on the contrary that it ranks among the most abundant diatom genera in the global ocean. The most likely explanation for this apparent discrepancy is that traditional collection and identification methods were unable to detect and taxonomically resolve such small-sized species. Flow cytometric analyses do not resolve diatom taxonomy and only detect these small diatoms as undetermined nanoplanktonic eukaryotes, while the diagnostic pigments approach54 usually attributes all fucoxanthin to the microphytoplankton size-class, which is obviously erroneous for the DeWeX case study. Much like the application of flow cytometry was required to detect the significance of Prochlorococcus in the global ocean55, the advent of improved genomic sequencing performed on a global scale and systematic coupling of faster SEM techniques to regular optical microscopy should help to further resolve this diatom size class in coming years. But clearly, quantitative physiological rates will be needed to improve model parameterizations.
Diatom cell sizes, as well as their Si/C cellular quotas, are key variables controlling their sinking fluxes56,57. In the North Atlantic in 1989–1990, a nearly two-fold reduction in carbon flux to deep sediment traps occurred during two successive years following the spring diatom bloom7. In 1990, the spring bloom was dominated by the tiny pennate Nanoneis hasleae forming a bloom as large as 50,000 km2 visible from space43, while it was dominated by a large size chain-forming Chaetoceros assemblage in the previous year7. This was one of the first studies to document a direct effect of the community structure within the same PFT on the modulation of carbon export fluxes to depth. This scenario was consistent with the classical opposition between small solitary diatoms carrying no protective spines correlated to slower sinking rates and faster C remineralization (through either bacterial lysis or grazing) in the water column compared to larger chain-forming and/or spiny cells, prone to aggregation and fast sinking. The data collected during the DeWeX spring bloom from deep sediment traps adds, however, a further aspect to this scenario, demonstrating that they are able to sink out of the surface layer in aggregated form at high sinking rates contrary to the common assertion that they are more likely to be entirely remineralized within the microbial loop in the water column. There is, therefore, a need to better constrain sedimentation rates and aggregation processes from different sizes of diatoms. This focus probably needs to be extended to other nanoeukaryotic groups, such as Parmales, another elusive siliceous scale-bearing phytoplankton of 2–6 µm diameter, whose importance has likely been underestimated44. Nano-diatoms and Parmales are ballasted by their mineral casings, which should lead to a different fate in the water column with respect to other non-siliceous organisms of the same size-class. The possibility for picoplankton to escape the microbial loop and fuel export was already demonstrated by sediment trap data13, but to date it has not been considered extensively. We further propose that the diatom group represented in models as a single PFT should be redefined to better include various life strategies of survival and growth, such as in trait-based models, and that the nanophytoplankton group also needs to be connected to the siliceous pathway.
Another outcome of the modeling study is that traits of diatoms concerning their relationships to predators need to be further explored. Size-selective predation, which can include zoosporic parasitism32, rather than competition for resources may be a major selective pressure for diatoms, driving their ecological properties3. At least two other studies reporting spring bloom abundances of Minidiscus sp. in the Subarctic Pacific5 and in the Norwegian basin10 displayed similar context and pointed toward the same explanation. Both studies argued that despite generous supplies of nutrients, usually favorable to the growth of large cells, the net accumulation of small-sized diatoms could be a result of selective grazing pressure on larger cells and possibly linked to a lack of seed populations from coastal environments during the initial stages of the bloom. None of these studies, including ours, were able to precisely determine the interspecific predator-prey relationships between zooplankton groups and distinct diatom species. Indeed, determining trophic links in the natural environment remain a major challenge and constitutes a bottleneck in our understanding of marine planktonic ecosystems. Understanding of accessibility, vulnerability, prey-induced biases in the predator perception, and trophic relations with organisms other than copepods is still in its infancy and needs to be scaled up. The same is true about symbiotic interactions such as the one recently discovered between Minutocellus and a foraminifera. The advent of improved genomic sequencing and systematic coupling of faster SEM techniques to regular optical microscopy should help to further resolve this diatom size class in coming years.
Altogether, these observations challenge the common assumption that small (defined as >10 µm) to large-sized chain-forming diatoms are the usual initial bloomers in frontal areas and turbulent environments following winter fertilization2. We propose that Minidiscus and other overlooked minute diatom taxa (<5 µm) may be occasional major contributors to spring blooms in turbulent nutrient-rich environments of various coastal and offshore oceanic regions and that, upon aggregation, they may also contribute significantly to carbon export.
Satellite-derived surface chlorophyll a concentration was accessed at http://oceancolor.gsfc.nasa.gov (MODIS Aqua, 4 km, 8-days composite, level 3 product). Altimetry-derived geostrophic velocities (AVISO MADT, 1/4°, daily product) were extracted at http://www.aviso.altimetry.fr/. Both satellite-derived chlorophyll a and geostrophic velocities were time-averaged over periods corresponding to the DeWeX cruises leg 1 (03–21 February 2013) and leg 2 (05–24 April 2013) to produce the maps in Fig. 1.
DeWeX cell counts and taxonomical determination
Water samples (125 mL) were collected during the DeWeX cruises at the surface using Niskin bottles mounted on a CTD frame and immediately fixed with 0.4 mL acidified Lugol (100 g KI, 50 g I, 100 mL glacial acetic acid) and stored at 4 °C. Diatoms were identified and enumerated by light microscopy at X4400 and X8800 magnification depending on size on a Nikon TE-2000 microscope in 50 or 100 mL Utermöhl sedimentation chambers depending on diatom abundance. Large and numerically rare taxa were counted in the entire settling chamber, while smaller dominant taxa such as Minidiscus were determined by light microscopy at X 8800 by counting two cuve diameters (i.e., 1/33 of the cuve area), which amounts to a volume of 3 mL counted in the case of 100 mL sedimented samples and 1.5 mL in the case of 50 mL sedimented samples. Minidiscus trioculatus and comicus were taxonomically determined using an FEI Teneo SEM in one of the very-high abundance samples (station 99, leg 2, 24 April 2013). All similar looking small centrics subsequently observed by light microscopy were assumed to be Minidiscus. Data for microscopic diatom counts are available at http://www.obs-vlfr.fr/proof/ftpfree/dewex/db/data/DIATOMS/.
Semi-continuous automated flow cytometry
Phytoplankton cells were counted semi-continuously (one sample every hour during day time and every 2 h during night time) using an automated Cytosense flow cytometer (Cytobuoy, NL) connected to a continuous seawater flow-through pumped at 3 m depth. In order to ensure an accurate position and limit the distance traveled while analyzing the sample, the Cytosense pumped its sample from an isolated chamber of 300 mL filled in less than 30 s. The chamber was opened to the flow-through in between each analysis. The Cytosense instrument is specially adapted to detecting wide in situ phytoplankton wide size ranges and abundances. Each sample was driven toward the flow cell by a calibrated peristaltic pump running between <1 μL s−1 and 20 μL s−1 from which the volume analyzed was calculated. Every particle (cell) in suspension in the sample was then separated through a laminar flow thanks to a 0.2 µm filtered sheath fluid made of seawater and crossed a laser beam (Coherent, 488 nm, 20 mW). The instrument recorded various pulse shapes emitted while the cells were crossing the laser beam, resulting in: forward angle light scatter and sideward angle light scatter as well as red, orange, and yellow fluorescence bands in the size range 1–800 µm in width and a few mm in length for chain-forming cells. Laser scattering at small angles was collected by two distinct photodiodes to check for the sample core alignment. Two trigger levels on the red fluorescence were applied for distinction between highly concentrated and picophytoplankton and cyanobacteria groups (trigger level FLR 8 mV, sampling at a flow rate of 10 mL3 s−1 analyzing ~1 mL), and lower concentrated nano- and microphytoplankton (trigger level FLR 10 mV, at a flow rate of 10 mL3 s−1 analyzing ~5 mL). Different sets of 2D projections of the data were plotted in Cytoclus® software to manually gate the various phytoplankton groups. A combination of standard beads (PolyScience® Yellow Green Polystyrene 2 μm, 3 μm, 6 μm, 10 μm, and 20 μm diameter) was regularly analyzed to monitor the stability of the flow cytometer. The volume analyzed was weight calibrated.
Biogenic silica samples (2 L) were collected in the same Niskin bottles as cell counts and filtered onto 0.8 µm, 47 mm polycarbonate filters, dried at 60 °C for 24 h, and stored at room temperature. As samples were coastal and susceptible to receive large terrigeneous riverine inputs, they were analyzed in the laboratory following the three-step digestion method allowing for determination of biogenic silica corrected for the interference from lithogenic silica58. Data for biogenic silica concentrations are available at http://www.obs-vlfr.fr/proof/ftpfree/dewex/db/data/Si/. POC from water samples was analyzed on GF/F filters on a CHN elemental analyzer. Nutrient samples were stored in 20 mL polyethylene vials and immediately frozen at −20 °C until analysis. In the laboratory, the samples were analyzed by colorimetry on a Seal-Bran-Luebbe AutoAnalyzer AA3 HR59.
Trait-based model of phytoplankton strategists
A trait-based model of a phytoplankton community is used in the present study to explore hypotheses related to the occurrence of the massive Minidiscus bloom observed during the DeWeX 2013 campaign. Four types of phytoplankton strategists are represented according to their distinctive traits with regards to the available amounts of light and nutrients. The difference in these physiological traits between phytoplankton types is based on the Reynolds’ C-S-R classification30, and combination of these traits within a phytoplankton genus enables to determine its ecological niche. The main assumption of the Reynolds’ model is that a bloom of a given strategist occurs when the environmental conditions (light, nutrients) match its ecological niche. In the present study, the following four strategists are defined. SS-strategists are recurrent nutrient stress tolerant genera in high-light environments, such as the cyanobacteria Synechococcus that are constitutive members of the picophytoplankton in the Mediterranean Sea60. R-strategists optimally grow in low-light environments, but they require high nutrients in order to form bloom events. This group incorporates many genera of large diatoms with elongated body shapes such as Chaetoceros, Leptocylindrus, and Guinardia, all of which are genera observed during the spring leg of the DeWeX cruise (see Results section). In our study, R-strategists represent large diatoms with fast sinking rates generally observed during spring blooms at mid-latitudes. The last group is that of C-strategists (colonist genera). The physiological and morphological traits common to all genera of this group are small sizes in the nanoplanktonic spectra, flat cylindrical body shapes, and some fast-growing abilities when nutrients and light are abundant. The choice was made to divide C-strategists into two categories according to close traits: on the one hand, C1-strategists gather autotrophic flagellates and small-sized cryptophyceae that showed a marked presence during the spring leg of DeWeX cruise61, on the other hand, C2-strategist represents Minidiscus diatoms.
The two latter strategists, C1 and C2, are considered to be in the same range of size and to have similar traits62. In our model, some differences in traits are, however, assumed and they go beyond just a difference in a reliance or not on Si uptake for the C2- and C1-strategists. In particular, the C2-strategist is also considered more tolerant to light stress and less tolerant to nutrient stress than C1-strategists. These different abilities are based on knowledge of the spring succession of the phytoplankton community acquired by remote sensing63 and in situ observations64 in the northwestern Mediterranean Sea. These studies indicate that the first community to peak is generally diatoms from January to March followed several weeks later by a peak of nano-eukaryotes at the beginning of the stratification period when surface nutrients decrease. This is the usual pattern of the second stage of the bloom dominated by nano-eukaryotes (i.e., autotrophic flagellates, small cryptophyceae) that has typically been observed in various oceanic areas at mid-latitudes65,66. As suggested in the study of Marty et al. (2002)64, nano-eukaryotes would flourish during the time period for which successive events of stratification and destratification of the water column accompanied with weak injections of nutrients from deep layers occur. These differences in traits involve some emergent properties specific to each strategist such as, for example, their ability to grow differently depending on light and nutrient availability (see net photosynthetic growth rates in Supplementary Table 5). The choices of parameters in the trait-based model are crucial because they determine in fine the differences in functional traits between strategists. These choices have been made on the basis of different sources for usual allometric rules between functional traits and size or biovolume67,68 as well as different types of experimental data (field, laboratory assays). All the sources of parameters are indicated in the Suppl. Tables 1–4). For example, in the model, the half-saturation constant for nitrogen uptake increases with cell size while the photosynthetic efficiency (given in our model by the product of maximum quantum yield by Chl-specific absorption coefficient) decreases along cell size. In the same way, the choices of values for internal quotas are based on the observations of decreasing stoichiometric molar quotas along increasing cell volumes23,69 and from the use of the combined dataset of carbon cell contents vs. cell volume68,70.
However, when a new strategist (nano-diatoms in the present study) is incorporated in a trait-based model, it can often be difficult to allocate an accurate value to each of its physiological parameters owing to insufficient experimental information. In this case, the choices of the latter parameters are rather made on a qualitative idea that the trait of the strategist should be affected by environmental conditions. The difference in mortality rates between C1- and C2-strategists illustrates this point: the C1-strategist is assumed to represent a heterogeneous mix of nano-eukaryotes while the C2-strategist is a numerical representation of closely related species of one genus (i.e., Minidiscus). It is hypothesized that the mortality processes would have lower impacts on a heterogeneous community of plankton species because if one species collapses under a viral attack, for example, another one will arise, which enables the maintenance of numerous nano-eukaryotes. This mechanism is less likely to occur when a more homogeneous group such as Minidiscus is considered.
The type of trait-based model used here has been implemented in the biogeochemical modular numerical platform Eco3M71,72. The main characteristics of the model are mentioned hereafter. Each group of strategists is represented through several states variables of C, N, P, Si, and Chl contents and intracellular ratios can thus be computed at each time point allowing a non-redfieldian behavior of the model. They have the ability to take up dissolved inorganic and organic matter (i.e., mixotrophy) and to exude organic matter in order to adjust their stoichiometric internal requirements. A heterotrophic bacteria compartment is also considered for their ability to recycle organic matter into inorganic nutrients. Compartments of inorganic nutrients such as nitrate, phosphate, and silicic acid, as well as of organic matter are represented. Grazers and viruses of phytoplankton are not explicitly accounted for in the model but their processes of control are implicitly represented through mortality rates. All the mathematical formulations of processes are provided in details in Campbell et al.73.
Two numerical experiments were conducted to test the impact of these processes on the temporal dynamics of the different strategists. In particular, the mortality rate of R-strategists has been thus modulated upward (HCC: High Control Conditions) or downward (LCC: Low Control Conditions, standard simulation). In the HCC condition, the mortality rate corresponds to a disappearance of biomass by 25% d−1 while this rate is 10% d−1 in the LCC condition according to the study of Broglio et al.74. Given the short simulation time (40 days) variations in water temperature are neglected and no temperature-dependent processes are then considered in the model formulations. This assumption is corroborated by field observations that show temperature variation lower than 1 °C over the time of the simulated period21.
Model parameters characterizing each group of strategists and initial conditions in nutrients, living biomasses and organic matter are given in Supplementary Table 1. The standard run is launched from initial conditions in nutrients corresponding to those observed during DeWeX75 just at the end of the high convective episode (HCNC: High Convective Nutrient Condition) with [H4SiO4] = 7.72 µM, [NO3] = 8.40 µM, and [PO4] = 0.39 µM. Another run is launched with a reduced supply of nutrients characterizing reduced convective events (LCNC: Low Convective Nutrient Condition). These concentrations were chosen in the PERSEUS database for a low convective year (1990) and were set at [H4SiO4] = 2.10 µM, [NO3] = 2.66 µM, and [PO4] = 0.09 µM.
All simulations for the present study were performed under a constant irradiance of 300 W m−2 and a 12 h/12 h day/night cycle corresponding to the beginning of spring at mid-latitudes. Time step of the model is 300 s. The time of simulation is 40 days (roughly corresponding to the period of bloom during DeWeX) from the end27. From this last event, it is assumed that a light vs. nutrient optimal tradeoff exists for the phytoplankton bloom without any further variation of the physical environment (e.g., nutrient supplies, light variations). In this theoretical context, a trait-based model without considering any physical forcing (0D) can be used over the simulation period. Although useful to understand community dynamics, it does limit the model’s ability to correctly estimate in situ biomass levels because no loss terms due to physics are included, while considerable turbulent mixing and advection are known to occur in the area. Model parameters and initial conditions of state variables are presented in Supplementary Tables 1–5.
Sediment trap data
A short mooring line (~50 m long) was deployed at 42° 01′N–4° 48′E (depth of 2400 m). It was equipped with a Technicap PPS-3 sediment trap (collecting area of 0.125 m2, aspect ratio of 2.5, and 12 collecting cups) at 30 m above the seabed. The trap samples were collected with sampling interval between 15 and 23 days. Prior to deployment, the sampling bottles were filled with 0.45 µm filtered seawater containing sodium borate-buffered formalin to yield a final concentration of 5% formalin to prevent in situ microbial decomposition. Upon recovery, samples were stored in the dark at 4 °C76. 1 mL aliquots were filtered onto the center of 0.4 µm polycarbonate filter using a filtering funnel of 6 mm aperture, and carefully rinsed with DIW water, then dehydrated in increasing series of ethanol 30%, 50%, 70%, 80%, 90%, and 100% during 10 mn for each step. The samples were completely dried overnight, mounted on aluminum stubs with double sticky carbon tabs, and sputter-coated with gold for 10 min. The samples from each of the three available trap samples were analyzed with a FEI Teneo SEM.
Tara Oceans V9-18S rDNA metabarcodes
We used the global metabarcoding dataset (EBI accession number PRJEB16766) generated from the biological samples collected from 146 sampling locations during the Tara Oceans expedition12,77,78. We extracted ribotypes that were assigned to Minidiscus and Minutocellus from the three depths—surface, deep chlorophyll maximum, and MESO—within the different size-class filters (ranging from 0.8 to 2,000 µm, with the smallest fraction being 0.8 to 5 µm). The taxonomic assignations were done using PR2 reference database79 which has six reference sequences from Minidiscus trioculatus, 2 from Minidiscus sp., and one from an environmental sequence. From the photic zone, for Minidiscus, a total of 908 different V9 rDNA ribotypes (represented by 66,043 reads) were retrieved from the 81 communities representing the smallest size-fraction (0.8 to 5 µm), while for Minutocellus we retrieved 776 V9 rDNA ribotypes (represented by 35,108 reads) from the 118 communities representing the smallest size-fraction (0.8–5 µm). From the mesopelagic zone, a total of 49,239 reads for Minidiscus and 27,181 reads for Minutocellus were retrieved from 79 size-fractionated samples. Relative abundance was calculated with respect to total diatom reads and to total phytosynthetic reads, which comprised reads assigned to major phytoplanktonic groups80, namely Bacillariophyta, Chlorophyceae, Cryptophyta, Dictyochophyceae, Dinophyceae, Haptophyta, Mamiellophyceae, Pelagophyceae, and Raphidophyceae. Exponentiated Shannon-Weiner H’ diversity index was used as an estimate of diversity at each station. All the analyses were conducted using open source R version 3.3.1 (data and R-script available at https://figshare.com/s/6f3190905564f6c6e20c).
The authors declare that all data supporting the findings of this study are available within the paper and its Supplementary Information files.
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We thank the captain and crew of the R/V Le Suroît for their assistance during the DeWeX cruises. We acknowledge sponsorship from the MISTRALS-MERMEX, MOOSE, and Tara Oceans projects. Original data used in this work are available through the MERMEX-DEWEX (http://mistrals.sedoo.fr/MERMeX/) and MOOSE (Mediterranean Ocean Observing System on Environment; http://mistrals.sedoo.fr/MOOSE/), and Tara Oceans (https://www.ebi.ac.uk/metagenomics/) databases, cruises DeWeX-MERMEX 2013 LEG1 (https://doi.org/10.17600/13020010) and LEG2 (https://doi.org/10.17600/13020030), and sediment trap in the convection zone (https://doi.org/10.17882/45980). For SEM imagery, we thank the CINAM for the use of their SEM platform as well as the PICsL Imaging Facility, member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04). We thank Adriana Zingone for help in identifying Minidiscus species. C.B. acknowledges funding from the ERC Advanced Award Diatomite, EU FP7 grant MicroB3 (No. 287589), the Louis D Foundation, and the French Government Investissements d’Avenir programmes MEMO LIFE (ANR-10-LABX-54), PSL* Research University (ANR-1253 11-IDEX-0001-02), and OCEANOMICS (ANR-11-BTBR-0008). C.B. also thanks the Radcliffe Institute of Advanced Study at Harvard University for a scholar fellowship during the 2016-2017 academic year. This article is contribution #71 of Tara Oceans. M.T. and G.G. thank Dominique Lefèvre and Caroline Bachet for setting up the continuous seawater supply for automated multiparametric monitoring during the ship navigation and for help in the autonomous flow cytometer deployment. The project leading to this publication has received funding from European FEDER Fund under project 1166-39417.
The authors declare no competing interests.
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Leblanc, K., Quéguiner, B., Diaz, F. et al. Nanoplanktonic diatoms are globally overlooked but play a role in spring blooms and carbon export. Nat Commun 9, 953 (2018). https://doi.org/10.1038/s41467-018-03376-9
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