Fig. 2 | Nature Communications

Fig. 2

From: Life times of metastable states guide regulatory signaling in transcriptional riboswitches

Fig. 2

Structural integrity of caged dGsw121 before and after laser irradiation. a Secondary structures of dGsw121 in presence and absence of ligand, before photolysis (PA–T) and after photolysis to form the antiterminator conformations PAT(M) and PAT(I). Aptamer domain imino protons detectable by NMR are highlighted in orange. Antiterminator imino protons detectable by NMR are highlighted in green. b Overlay of 1D NMR spectra of caged dGsw121 before (black) and after (green) laser irradiation in absence (left) and presence (right) of 2 eq. of ligand  and combinations of overlay of 1H,15N -TROSY of dGsw121 in absence (−2′dG) or presence of 2 eq. ligand ( + 2′dG), and before and after ( + hν) laser irradiation. The spectra were recorded on 100 µM NMR samples with 6 eq. of Mg2+ at 800 MHz and 298 K. Characteristic imino proton signals to monitor the formation of PAT are highlighted in green. Characteristic imino proton signals to monitor aptamer dissociation in the presence of ligand are highlighted in red