Fig. 4 | Nature Communications

Fig. 4

From: Circular DNA elements of chromosomal origin are common in healthy human somatic tissue

Fig. 4

EccDNA validation and DNA deletion. a Gel images for a validated subset (n = 9) of eccDNAs by outward PCR (blue arrows), inward PCR (black arrows), gel electrophoresis, and Sanger sequencing. EccDNAs are named according to gene content; black boxes, exons. Template: T1–T16, muscle B1–B16 leukocyte, phi29 (φ) amplified eccDNA, GD genomic DNA, NTC nontemplate control. Sequenced PCR products are in boxes and alignment of resultant sequence are red and blue lines. b Confirmed verification of DAZ4exon 18 deletion and c [DAZ4circle exon 18] at identical coordinates within the DAZ4 locus by inward and outward PCR (oligos arrows, inward red, outward, blue)

Back to article page