Fig. 1 | Nature Communications

Fig. 1

From: Circular DNA elements of chromosomal origin are common in healthy human somatic tissue

Fig. 1

Circle-Seq method for mapping of eccDNA. a Leukocyte and muscle samples from 16 healthy subjects (T1–T16, n = 16). b Purification of eccDNA through column separation, exonuclease treatment, and rolling-circle amplification. c Detection of eccDNA based on structural-read variants and coverage (soft-clipped, split, red; concordant, gray; and discordant reads, blue). d Read coverage display (log-scale) at the titin gene, TTN, from muscle samples. e EccDNA from T5 and T6 illustrated (black boxes, exons), outward PCR validation (blue arrows), inward PCR (black arrows), and gel-image of T5 and T6 PCR products next to controls: GD, human genomic DNA; φ, phi29-amplified eccDNA sample without detected [TTNcircle]. f Sequence of [TTNcircle exon 44-52] T6 PCR product at junction